A5006623165- Molecular Sensors and Ion Detection
- Sulfur Compounds in Biology
- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- Luminescence and Fluorescent Materials
- Nitric Oxide and Endothelin Effects
- Nanocluster Synthesis and Applications
- Optical Network Technologies
- Advanced Photonic Communication Systems
- Nuclear Structure and Function
- RNA Interference and Gene Delivery
- Advanced Fluorescence Microscopy Techniques
- Lanthanide and Transition Metal Complexes
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced Nanomaterials in Catalysis
- Ion-surface interactions and analysis
- Metal-Organic Frameworks: Synthesis and Applications
- Advanced Chemical Sensor Technologies
- Cardiac Arrest and Resuscitation
- Carbon Nanotubes in Composites
- Semiconductor materials and devices
- Advanced Fiber Laser Technologies
- Traumatic Brain Injury and Neurovascular Disturbances
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Dalian Institute of Chemical Physics
2022-2025
Chinese Academy of Sciences
2022-2025
University of Chinese Academy of Sciences
2019-2025
Nanjing Medical University
2019-2025
Nanjing Jiangning Hospital
2025
Lanzhou University
2022-2024
University of Oxford
2023
John Wiley & Sons (United Kingdom)
2023
Hudson Institute
2023
Diagnosis and early assessment of the treatment response rheumatoid arthritis (RA) necessitates a reliable bioanalytical method for rapid, sensitive, specific detection hypochlorous acid (HOCl) biomarker in inflammatory diseases. Herein, two fluorescence probes, Probe-1 Probe-2 are developed quantitative monitoring visualization response-related HOCl levels vitro vivo. In presence HOCl, "OFF-ON" is obtained both probes as result HOCl-triggered C=N bond cleavage reaction. feature rapid (<4...
An effective bioanalytical method for rapid, sensitive, specific, and in situ sensing of nitric oxide (NO) is the key further unveiling biological functions this gasotransmitter molecule vitro vivo. In contribution, a new fluorescence probe imaging NO live systems was developed. The probe, FP-NO, designed by exploring novel mechanism, i.e., rotation N–N single bond coumarin derivative. FP-NO prepared incorporating recognition unit, thiosemicarbazide moiety into fluorophore. weakly...
Significance Although many efforts have been devoted to localize and monitor cancer progression, it is still difficult precisely evaluate the development of cancers for efficient targeted treatments. Herein, we demonstrate a straightforward strategy using in situ self-assembly gold nanocluster–DNA complexes. In combination with tumor suppressor gene-phosphatase tensin homolog (PTEN), nanocluster-PTEN complexes could realize bioimaging identification simultaneously inhibit effect relevant...
The development of new methods for rapid and effective detection bisulfite (HSO3–) in food samples imaging HSO3– intake animals is significant importance due to the key roles quality assurance community health. In this work, a responsive fluorescence probe, EQC, reported quantitative visualization animals. Upon addition HSO3–, UV–vis absorption red emission EQC were significantly decreased within 120 s. changes spectra rationalized by theoretical computations. proposed reaction mechanism...
Key roles of bisulfite (HSO3-) in food quality assurance and human health necessitate a reliable analytical method for rapid, sensitive, selective detection HSO3-. Herein, new red-emitting ratiometric fluorescence probe, BIQ, is reported sensitive HSO3- samples live animals. Probe BIQ recognizes via 1,4-nucleophilic addition reaction. As result this specific reaction, emission intensities at 625 475 nm are dramatically changed, allowing the model an aqueous solution. The obvious changes...
Abstract Intracellular proteome aggregation is a ubiquitous disease hallmark with its composition associated pathogenicity. Herein, this work reports on cell‐permeable photosensitizer (P8, Rose Bengal derivative) for selective photo induced proximity labeling and crosslinking of cellular aggregated proteome. identified out common scaffolds unique intrinsic binding affinity to various protein aggregates driven by the hydrophobic effect. Further acetylation permeabilizes selectively image,...
We report the development of a new chromogenic and fluorogenic probe for detection HOCl in zebrafish, mice with arthritis environmental water samples.
Covalent-type probes or sensors have been seldom reported for aggregated proteins. Herein, we a series of covalent solvatochromic to selectively modify and detect proteomes through the Schiff base reaction. Such modification was discovered by serendipity using P1 probe with an aldehyde functional group, exhibiting enhanced fluorescence intensity unusually large blue shift upon protein aggregation. Supported biochemical mass spectrometry results, identified that this can lysine residue...
Abstract Synthetic fluorescent protein chromophores have been reported for their singlet state fluorescence properties and applications in bioimaging, but rarely the triplet chemistries. Herein, we enabled photo‐sensitizing photo‐crosslinking through rational modulations. Extension of molecular conjugation introduction heavy atoms promoted generation reactive oxygen species. Unlike other photosensitizers, these selectively photo‐crosslinked aggregated proteins uncovered interactome profiles....
Abstract Photosensitizing fluorescent proteins (FP) (e.g. KillerRed) have been shown not capable of photo‐catalytic protein proximity labeling for downstream proteomic profiling applications. To acquire such a function, FP chromophores are engineered in 12 × combinatorial matrix synthetic analoges, achieving up to 1000 fold enhancement reactive oxygen species (ROS) production compared the natural FPs. with larger dipole moments exhibit higher ROS yield toward labeling. By conjugating...
<title>Abstract</title> Objective To establish a nomogram prediction model for the patients with prolonged disorders of consciousness (PDOC) caused by brain injury at six months based on behavioral scale scores, neuroelectro-physiological techniques and hypothalamic-pituitary hormone levels. Methods The clinical data PDOC who were first diagnosed hospitalized in Department Rehabilitation Medicine Affiliated Jiangning Hospital Nanjing Medical University from March 2023 to July 2024 collected...
Most bioorthogonal photouncaging reactions preferentially occur in polar environments to accommodate biological applications the aqueous cellular milieu. However, they are not precisely designed chemically adapt diverse microenvironments of cell. Herein, we report a hydrophobic reaction with tailored photolytic kinetics toward solvent polarity. Structural modulations aminobenzoquinone-based photocage reveal impact cyclic ring size, steric substituent, and electronic substituent on individual...
Abstract The development of effective bioanalytical methods for rapid, sensitive and specific detection HOCl in vitro vivo plays a key role better understanding the roles this molecule normal diseased conditions, but remains challenging due to highly reactive nature complicated biological conditions. In work, new fluorescence probe, PQI , was developed monitoring level samples. easily synthesized by one‐step condensation reaction. Upon addition HOCl, significant changes absorption spectra...
A coumarin–Cu<sup>2+</sup> ensemble based fluorescent chemosensor was developed for the selective detection of cysteine in aqueous media and live cells.
Abstract Molecular rotor‐based fluorophores (RBFs) activate fluorescence upon increase of micro‐viscosity, thus bearing a broad application promise in many fields. However, it remains challenge to control how RBFs responds viscosity changes. Herein, we demonstrate that the formation and regulation intramolecular hydrogen bonds excited state could modulate their rotational barrier, leading rational can be activated by micro‐viscosity. Based on this strategy, series were developed based...
Bioaccumulation of nanoplastic particles has drawn increasing attention regarding environmental sustainability and biosafety. How interact with the cellular milieu still remains elusive. Herein, we exemplify a general approach to profile composition "protein corona" interacting nanoparticles via photocatalytic protein proximity labeling method. To enable proteome particles, iodine-substituted BODIPY (I-BODIPY) is selected as photosensitizer covalently conjugated onto amino-polystyrene model...
Quantitative detection and visualization of hypochlorous acid (HOCl) in biological samples has emerged as a hot topic biochemical research because the important role this biomolecule live organisms. In contribution, novel 1,8-naphthalimide-based fluorescence probe, DNPH-NA, was designed synthesized for HOCl aqueous solutions systems. presence HOCl, significant changes absorption spectra were observed, which allow quantitative PBS buffer. The limit determined to be 50 nM. response DNPH-NA...
BackgroundMicroRNA (miRNA) therapeutics are a promising approach to cancer treatment. However, this method faces considerable challenges achieve tissue-specific, efficient, and safe delivery of miRNAs in vivo.MethodsHerein, we developed miRNA system based on the situ self-assembly Au-miRNA nanocomplexes (Au-miRNA NCs). Within microenvironment, constructed self-assembled NCs by coincubating gold salt tumor suppressor mimics, such as let-7a, miRNA-34a, miRNA-200a.FindingsThe vitro experiments...