A5038700923- Plant Physiology and Cultivation Studies
- Plant Pathogens and Fungal Diseases
- Plant Reproductive Biology
- Plant Pathogenic Bacteria Studies
- Horticultural and Viticultural Research
- Fungal Plant Pathogen Control
- Plant and Fungal Interactions Research
- Genetic diversity and population structure
- Postharvest Quality and Shelf Life Management
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Resistance
- Psidium guajava Extracts and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Plant Gene Expression Analysis
- Banana Cultivation and Research
- Nuts composition and effects
- Plant Growth Enhancement Techniques
- Agriculture and Biological Studies
- Aquatic Invertebrate Ecology and Behavior
- Fish Ecology and Management Studies
- Research on scale insects
- Genomics and Phylogenetic Studies
- Animal Genetics and Reproduction
- Phytoplasmas and Hemiptera pathogens
- Plant tissue culture and regeneration
Istituto Sperimentale per la Frutticoltura
2003-2019
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria
2012-2019
The International Peach Genome Initiative reports the high quality draft genome sequence of peach (Prunus persica). They also resequenced ten additional P. persica accessions, as well those Prunus ferganensis, kansuensis, davidiana and mira. Rosaceae is most important fruit-producing clade, its key commercially relevant genera (Fragaria, Rosa, Rubus Prunus) show broadly diverse growth habits, fruit types compact diploid genomes. Peach, a species, one best genetically characterized deciduous...
The availability of the peach genome sequence has fostered relevant research in and related Prunus species enabling identification genes underlying important horticultural traits as well development advanced tools for genetic genomic analyses. first release (Peach v1.0) represented a high-quality WGS (Whole Genome Shotgun) chromosome-scale assembly with high contiguity (contig L50 214.2 kb), large portions mapped sequences (96%) base accuracy (99.96%). aim this work was to improve quality by...
We isolated and sequenced 26 microsatellites from two genomic libraries of peach cultivar 'Redhaven', enriched for AC/GT AG/CT repeats, respectively. For 17 these microsatellites, it was possible to demonstrate Mendelian inheritance. Microsatellite polymorphism assayed in 50 nectarine cultivars. Of the 1300 PCRs carried out, all but produced amplified products expected size. All were polymorphic, showing 2-8 alleles per locus. Heterozygosity ranged 0.04-0.74 (mean 0.47); discrimination power...
Peach was domesticated in China more than four millennia ago and from there it spread world-wide. Since the middle of last century, peach breeding programs have been very dynamic generating hundreds new commercial varieties, however, most cases such varieties derive a limited collection parental lines (founders). This is one reason for observed low levels variability gene pool, implying that knowledge extent distribution genetic critical to allow choice adequate parents confer enhanced...
Nectarines play a key role in peach industry; the fuzzless skin has implications for consumer acceptance. The peach/nectarine (G/g) trait was described as monogenic and previously mapped on chromosome 5. Here, position of G locus delimited within 1.1 cM interval (635 kb) based linkage analysis an F2 progeny from cross 'Contender' (C, peach) x 'Ambra' (A, nectarine). Careful inspection genes annotated corresponding genomic sequence (Peach v1.0), coupled with variant discovery, led to...
Abstract This article documents the addition of 220 microsatellite marker loci to Molecular Ecology Resources Database. Loci were developed for following species: Allanblackia floribunda, Amblyraja radiata, Bactrocera cucurbitae, Brachycaudus helichrysi, Calopogonium mucunoides, Dissodactylus primitivus, Elodea canadensis, Ephydatia fluviatilis, Galapaganus howdenae howdenae, Hoplostethus atlanticus, Ischnura elegans, Larimichthys polyactis, Opheodrys vernalis, Pelteobagrus fulvidraco,...
Peach (Prunus persica (L.) Batsch) is a major temperate fruit crop with an intense breeding activity. Breeding facilitated by knowledge of the inheritance key traits that are often quantitative nature. QTLs have traditionally been studied using phenotype single progeny (usually full-sib progeny) and correlation set markers covering its genome. This approach has allowed identification various genes but limited small numbers individuals used narrow transect variability analyzed. In this...
A wide inventory of molecular markers is nowadays available for individual fingerprinting. Microsatellites, or simple sequence repeats (SSRs), play a relevant role due to their relatively ease use, abundance in the plant genomes, and co-dominant nature, together with availability primer sequences many important agricultural crops. Microsatellites long-core motifs are more easily scored were adopted long ago human genetics but they developed only few crops, Prunus species not among them. In...
A linkage map was obtained using a BC1 progeny (Prunus persica x (P. P ferganensis)). The is composed of 109 loci (74 RFLPs, 17 SSRs, 16 RAPDs, and two morphological traits) distributed in 10 groups. Loci, segregating five different ratios, were integrated the with JoinMap 2.0 software. covers 521 cM peach genome. average distance between adjacent 4.8 cM. Two monogenic traits, flesh adhesion (F/f) leaf glands (E/e), placed on map. Thirty-two common saturated Prunus allowed comparative...
The purpose of the present study was to identify tight linkage between important agronomic traits and molecular markers with aim using them in Marker Assisted Selection (MAS) peach breeding programs at ISF. Quantitative trait loci (QTLs) influencing several were identified a BC1 population issued from cross selection Prunus persica (IF 7310828) used as female recurrent parent an accession related wild species P. ferganensis carrying source resistance powdery mildew. One hundred nine (RFLPs,...
Peach (Prunus persica) is one of the most important temperate fruit trees in world, based on its production and cultivated area. Consumer acceptance principal objective multiple breeding programs it dependent many factors. Among these factors, an role played by soluble solids content (SSC) postharvest performance represented mealiness (M) susceptibility as a chilling injury disorder. Additionally, major maturity date (MD) QTL has been reported to have pleiotropic effect both M SSC. The aim...
A total of 101 Pseudomonas syringae pv. strains, obtained from international culture collections or isolated diseased tissues herbaceous and woody plant species, were assessed by repetitive PCR using the BOX primer, for presence syrB gene. Representative strains also tested pathogenicity to lilac, pear, peach, corn bean, as well virulence lemon zucchini fruits. The unweighted pair‐group method arithmethic averages analysis (UPGMA) genomic fingerprints revealed 17 different patterns which...
Abstract Twenty‐one expressed sequence tag–simple repeat (EST–SSR) markers were developed in peach from a mesocarp cDNA library. Eighteen of them gave successful amplification 22 genotypes and produced one to three alleles each with an average 1.8 per locus. The value expected observed heterozygosities was 0.24 0.20, respectively. All the primers other six Prunus species (almond, apricot, sweet cherry, Japanese plum, European plum ferganensis ).
The KNOTTED-like (KNOX) genes encode homeodomain transcription factors and regulate several processes of plant organ development. peach (Prunus persica L. Batsch) genome was found to contain 10 KNOX members (KNOPE genes); six them were experimentally located on the Prunus reference map class 1 KNOPE1 link a quantitative trait locus (QTL) for internode length in peach×Ferganensis population. All KNOPE differentially transcribed internodes growing shoots; mRNA abundance decreased progressively...
ISHS XXV International Horticultural Congress, Part 11: Application of Biotechnology and Molecular Biology Breeding - Gene Expression Breeding, Genome Analysis. GENETIC LINKAGE MAP AND QTL ANALYSIS IN PEACH
Bacterial spot caused by Xanthomonas arboricola pv. pruni (Xap) is a major threat to Prunus species worldwide. The molecular mechanisms of peach resistance Xap during early leaf infection were investigated RNA-Seq analysis two persica cultivars, 'Redkist' (resistant), and 'JH Hale' (susceptible) at 30 minutes, 1 3 hours-post-infection (hpi). Both cultivars exhibited extensive modulation gene expression mpi, which reduced significantly hpi, increasing again hpi. Overall, 714 differentially...
Abstract In order to point out possible genetic differences, 37 Pseudomonas avellanae strains isolated from northern Greece, Italy and two different areas of central Italy, were assessed by means Repetitive PCR (rep‐PCR) using Enterobacteriaceae Intergenic Consensus (ERIC) primers. After amplifications, the DNA fingerprints revealed three major groups. The Greece those similar showing, in common, products ranging approximately 250 1100 base pairs. However, can be differentiated presence...
A linkage map was obtained using a BC1 progeny (Prunus persica × (P. P. ferganensis)). The is composed of 109 loci (74 RFLPs, 17 SSRs, 16 RAPDs, and two morphological traits) distributed in 10 groups. Loci, segregating five different ratios, were integrated the with JoinMap 2.0 software. covers 521 cM peach genome. average distance between adjacent 4.8 cM. Two monogenic traits, flesh adhesion (F/f) leaf glands (E/e), placed on map. Thirty-two common saturated Prunus allowed comparative...