A5073966267- Antimicrobial Resistance in Staphylococcus
- Antimicrobial Peptides and Activities
- Bacterial biofilms and quorum sensing
- Biochemical and Structural Characterization
- Skin and Cellular Biology Research
- Bacterial Genetics and Biotechnology
- Ocular Infections and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Corneal Surgery and Treatments
- RNA and protein synthesis mechanisms
- Advanced Glycation End Products research
- Toxin Mechanisms and Immunotoxins
- Immune Response and Inflammation
- Angiogenesis and VEGF in Cancer
- Ocular Surface and Contact Lens
- Wnt/β-catenin signaling in development and cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Calpain Protease Function and Regulation
- Bacterial Infections and Vaccines
- Herpesvirus Infections and Treatments
- Clostridium difficile and Clostridium perfringens research
- Seaweed-derived Bioactive Compounds
- Bee Products Chemical Analysis
- Atherosclerosis and Cardiovascular Diseases
- Escherichia coli research studies
University of California, Davis
2010-2022
Rockefeller University
2022
University of California, Berkeley
2022
New York University
2016-2021
Cleveland Clinic Lerner College of Medicine
2016-2019
Case Western Reserve University
2016-2019
Cleveland Clinic
2016-2019
Cleveland Eye Clinic
2018-2019
Czech Academy of Sciences, Institute of Microbiology
2017
Western Human Nutrition Research Center
2012
Rationale: A high-fat diet accompanied by hypertriglyceridemia increases an individual’s risk for development of atherosclerosis. An early event in this process is monocyte recruitment through binding to vascular cell adhesion molecule 1 (VCAM-1) upregulated on inflamed arterial endothelium. Diets high polyunsaturated fatty acids (PUFAs) may provide athero-protection ameliorating effect. Objective: We investigated the acute regulation VCAM-1 expression human aortic endothelial cells (HAEC)...
A rise in postprandial serum triglycerides (PP-sTG) can potentiate inflammatory responses vascular endothelial cells (ECs) and thus serves as an independent risk factor for predicting increased cardiovascular morbidity. We examined triglyceride-rich lipoproteins (PP-TGRLs) subjects ranging from normal to hypertriglyceridemic their capacity alter EC acute responses. Cultured human aortic ECs (HAECs) were conditioned with PP-TGRLs isolated at the peak after a moderately high-fat meal. VLDL...
Endothelial cells (EC) derived from embryonic stem (ESC) require additional functional characterization before they are used as a cell therapy in order to enhance their potential for engraftment and proliferation. We explore several physiologically relevant functions of ESC-derived EC (ESC-EC), such its capacity produce nitric oxide (NO), regulate permeability, activate express surface molecules the recruitment leukocytes response inflammatory stimuli, migrate grow new blood vessels, lay...
Staphylococcus aureus is responsible for various diseases in humans, and recurrent infections are commonly observed. S. produces an array of bicomponent pore-forming toxins that target kill leukocytes, known collectively as the leukocidins. The contribution these leukocidins to impair development anti–S. adaptive immunity facilitate reinfection unclear. Using a murine model bacteremia, we demonstrate infection with leukocidin mutant results increased levels antibodies compared mice infected...
The Clp protease system is a promising, noncanonical drug target against
Overcoming the species specificity of MRSA toxin LukAB with a novel mouse model establishes role for in vivo.
Staphylococcus aureus is a Gram-positive opportunistic pathogen that causes superficial and invasive infections in the hospital community. High mortality from infection emphasizes need for improved methods prevention treatment. Although S. possesses an arsenal of virulence factors contribute to evasion host defenses, few studies have examined long-term humoral B-cell responses. Adults with acute-phase skin soft tissue were recruited; blood samples obtained; isolates, including...
Leukocidin ED (LukED) is a bicomponent pore-forming toxin produced by Staphylococcus aureus that lyses host cells targeting the chemokine receptors CC receptor type 5 (CCR5), CXCR1, CXCR2, and DARC. In addition to its role as for LukED, CCR5 major coreceptor primary isolates of human immunodeficiency virus 1 (HIV-1) has been extensively studied. To compare how LukED HIV-1 target CCR5, we analyzed their respective abilities use CCR5/CCR2b chimeras mediate cytotoxicity entry. These analyses...
Staphylococcus aureus (Sa) is the leading cause of a variety bacterial infections ranging from superficial skin to invasive and life threatening diseases such as septic bacteremia, necrotizing pneumonia, endocarditis. The success Sa human pathogen contributed its ability adapt different environments by changing expression, production, or secretion virulence factors. Although immune evasion well-studied, regulation factors under nutrient growth conditions still not well understood. Here, we...
Anaplasma phagocytophilum is an obligate intracellular bacterium that has evolved mechanisms to hijack polymorphonuclear neutrophil (PMN) receptors and signaling pathways bind, infect, multiply within the host cell. E-selectin upregulated during inflammation a requisite endothelial receptor supports PMN capture, rolling, activation of integrin-mediated arrest. Ligands expressed by mediate binding endothelium via include sialyl Lewis x (sLe )-expressing ligands such as P-selectin glycoprotein...
Staphylococcus aureus infection is a major public health threat in part due to the spread of antibiotic resistance and repeated failures develop protective vaccine. Infection associated with production virulence factors that include exotoxins attack host barriers cellular defenses, such as leukocidin (Luk) family bicomponent pore-forming toxins. To investigate structural basis antibody-mediated functional inactivation Luk toxins, we generated panel murine monoclonal antibodies (MAbs)...
Unbiased identification of individual immunogenic B-cell epitopes in major antigens a pathogen remains technology challenge for vaccine discovery. We therefore developed platform rapid phage display screening deep recombinant libraries consisting as few one antigen. Using the bicomponent pore-forming leukocidin (Luk) exotoxins Staphylococcus aureus prototype, we randomly fragmented and separately ligated hemolysin gamma A (HlgA) LukS genes into custom-built system, termed pComb-Opti8. Deep...
Previous studies have shown that inter- and intramuscular fat, together, relate with knee osteoarthritis symptoms disease features. However, the manual methods of fat muscle segmentation from MR images are tedious limit feasibility examining intra-muscular within separate groups thigh. For this reason, it remains unclear whether specific flexor, extensor, adductors or abductors contribute differentially to trajectories change in symptoms. To investigate how thigh predict faster progression...