A5019384731- Cell Adhesion Molecules Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Platelet Disorders and Treatments
- Immune Response and Inflammation
- Atherosclerosis and Cardiovascular Diseases
- Blood properties and coagulation
- Cellular Mechanics and Interactions
- Protease and Inhibitor Mechanisms
- S100 Proteins and Annexins
- Immune cells in cancer
- Wound Healing and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Ultrasound and Hyperthermia Applications
- Chemokine receptors and signaling
- Photoacoustic and Ultrasonic Imaging
- Blood disorders and treatments
- Angiogenesis and VEGF in Cancer
- Ion Channels and Receptors
- Caveolin-1 and cellular processes
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Signaling Pathways in Disease
University of California, Davis
2016-2025
National Institute of Standards
2019
National Institute of Standards and Technology
2019
Information Technology Laboratory
2019
Baylor College of Medicine
1996-2015
Houston Methodist
2009-2015
Center for Human Genetics
2015
The University of Texas Health Science Center at Houston
2015
Methodist Hospital
2015
Peking Union Medical College Hospital
2015
To differentiate the unique and overlapping functions of LFA-1 Mac-1, LFA-1-deficient mice were developed by targeted homologous recombination in embryonic stem cells, neutrophil function was compared vitro vivo with Mac-1-deficient, CD18-deficient, wild-type mice. exhibit leukocytosis but do not develop spontaneous infections, contrast to CD18-deficient After zymosan-activated serum stimulation, neutrophils demonstrated activation, evidenced up-regulation surface did show increased adhesion...
Neutrophil abscess formation is critical in innate immunity against many pathogens. Here, the mechanism of neutrophil was investigated using a mouse model Staphylococcus aureus cutaneous infection. Gene expression analysis and vivo multispectral noninvasive imaging during S. infection revealed strong functional temporal association between recruitment IL-1β/IL-1R activation. Unexpectedly, neutrophils but not monocytes/macrophages or other MHCII-expressing antigen presenting cells were...
Since macromolecular permeability between endothelial cells is regulated by tight junctions (zonula occludens), we wished to determine whether they also regulate neutrophil transendothelial migration. HUVEC monolayers, a commonly used model for studying leukocyte transmigration, were characterized using electric cell substrate impedance sensing and transmission electron microscopy. We show that culture medium containing growth supplement (50 microg/ml) was sufficient necessary the...
Abstract On inflamed endothelium selectins support neutrophil capture and rolling that leads to firm adhesion through the activation binding of β2 integrin. The primary mechanism cell involves ligation chemotactic agonists presented on endothelium. We have pursued a second involving signal transduction while neutrophils tether in shear flow. assessed whether E-selectin led arrest via integrins. Neutrophils were introduced into parallel plate flow chamber having as substrate an L monolayer...
Emigration of leukocytes at sites inflammation is initiated by the selectin family carbohydrate-binding adhesion molecules. Molecular crossbridges initiate rolling cells along vascular endothelium where chemokines such as IL-8 and platelet activating factor (PAF) may be presented to their receptors on leukocyte surface resulting in cell stimulation. Integrin activation appears a requirement for subsequent localization diapedesis into tissue. Several recent reports have demonstrated that...
Carbohydrate–protein bonds interrupt the rapid flow of leukocytes in circulation by initiation rolling and tethering at vessel walls. The cell surface carbohydrate ligands are glycosylated proteins like mucin P-selectin glycoprotein ligand-1 (PSGL-1), which bind ubiquitously to family E-, P-, L-selectin membranes endothelium. current view is that carbohydrate–selectin dissociate a few times per second, unbinding rate increases weakly with force. However, such studies have provided little...
Background— Monocyte activation and migration into the arterial wall are key events in atherogenesis associated with hypercholesterolemia. CD11c/CD18, a β 2 integrin expressed on human monocytes subset of mouse monocytes, has been shown to play distinct role monocyte adhesion endothelial cells, but regulation CD11c hypercholesterolemia its unknown. Methods Results— Mice genetically deficient were generated crossbred apolipoprotein E (apoE) −/− mice generate /apoE mice. Using flow cytometry,...
Background Post-arthroplasty infections represent a devastating complication of total joint replacement surgery, resulting in multiple reoperations, prolonged antibiotic use, extended disability and worse clinical outcomes. As the number arthroplasties U.S. will exceed 3.8 million surgeries per year by 2030, post-arthroplasty is projected to increase over 266,000 annually. The treatment these exhaust healthcare resources dramatically medical costs. Methodology/Principal Findings To evaluate...
Naive T cells are selectively recruited from the blood into peripheral lymph nodes during lymphocyte recirculation. L-selectin, a lectin-like receptor, mediates initial attachment of lymphocytes to high endothelial venules (HEV) in nodes. A subsequent step involving activation beta 2 integrins has been proposed facilitate firm adhesion, but activating signals poorly understood. We report here that either antibody-mediated cross-linking L-selectin on human or treatment with GlyCAM-1, an...
Heat shock protein (HSP) 60 is a mitochondrial and cytosolic protein. Previously, we reported that HSP60 doubled in end-stage heart failure, even though levels of the protective HSP72 were unchanged. Furthermore, observed acute injury adult cardiac myocytes resulted movement to plasma membrane. We hypothesized inflammatory state failure would cause translocation membrane this provide pathway for injury. Two models used test hypothesis: 1) rat model 2) human explanted failing hearts. found...
Objective— Atherosclerosis is associated with monocyte adhesion to the arterial wall that involves integrin activation and emigration across inflamed endothelium. Involvement of β 2 -integrin CD11c/CD18 in atherogenesis was recently shown dyslipidemic mice, which motivates our study its inflammatory function during hypertriglyceridemia humans. Methods Results— Flow cytometry blood from healthy subjects fed a standardized high-fat meal revealed at 3.5 hours postprandial, CD11c surface...
The mechanisms that mediate durable protection against Staphylococcus aureus skin reinfections are unclear, as recurrences common despite high antibody titers and memory T cells. Here, we developed a mouse model of S. reinfection to investigate protective responses. In contrast with WT mice, IL-1β-deficient mice exhibited poor neutrophil recruitment bacterial clearance during primary infection was rescued secondary challenge. γδ cells from skin-draining LNs utilized compensatory...
T cell cytokines contribute to immunity against Staphylococcus aureus , but the predominant subsets involved are unclear. In an S. skin infection mouse model, we found that IL-17 response was mediated by γδ cells, which trafficked from lymph nodes infected induce neutrophil recruitment, proinflammatory IL-1α, IL-1β, and TNF, host defense peptides. RNA-seq for TRG TRD sequences in revealed a single clonotypic expansion of encoded complementarity-determining region 3 amino acid sequence, could...
Mesenchymal stem cell therapies promote wound healing by manipulating the local environment to enhance function of host cells. Aggregation mesenchymal cells (MSCs) into three-dimensional spheroids increases survival and augments their anti-inflammatory proangiogenic potential, yet there is no consensus on preferred conditions for maximizing spheroid in this application. The objective study was optimize forming MSC that simultaneously nature. We applied a design experiments (DOE) approach...
Changes in the microbiota composition are associated with many human diseases, but factors that govern strain abundance remain poorly defined. We show a commensal Escherichia coli and pathogenic Salmonella enterica serovar Typhimurium isolate both utilize nitrate for intestinal growth, each accesses this resource distinct biogeographical niche. Commensal E. utilizes epithelial-derived nitrate, whereas niche occupied by S. is derived from phagocytic infiltrates. Surprisingly, avirulent was...
Intercellular junctions have long been considered the main sites through which adherent neutrophils (PMNs) penetrate endothelium. Tight (TJs; zonula occludens) are most apical component of intercellular cleft and they form circumferential belt-like regions intimate contact between adjacent endothelial cells. Whether PMN transmigration involves disruption TJ complex is unknown. We report here that TJs appear to remain intact during adhesion transmigration. Human umbilical vein cell (HUVEC)...
ABSTRACT Enterococcus faecalis aggregation substance (AS) mediates efficient bacterium-bacterium contact to facilitate plasmid exchange as part of a bacterial sex pheromone system. We have previously determined that AS promotes direct, opsonin-independent binding E. human neutrophils (PMNs) via complement receptor type 3 and other receptors on the PMN surface. now examined functional consequences this bacterium-host cell interaction. AS-bearing was phagocytosed internalized by PMNs,...