A5060031385- Probiotics and Fermented Foods
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- DNA Repair Mechanisms
- Molecular Communication and Nanonetworks
- Photoreceptor and optogenetics research
- CRISPR and Genetic Engineering
- Receptor Mechanisms and Signaling
- Gene Regulatory Network Analysis
- Advanced Fluorescence Microscopy Techniques
- S100 Proteins and Annexins
- Clostridium difficile and Clostridium perfringens research
- Retinal Development and Disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cellular Mechanics and Interactions
- Protein Kinase Regulation and GTPase Signaling
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Wnt/β-catenin signaling in development and cancer
- Cell Adhesion Molecules Research
- Hippo pathway signaling and YAP/TAZ
- Genetics and Neurodevelopmental Disorders
- Gut microbiota and health
University of California, Davis
2018-2023
Changes in the microbiota composition are associated with many human diseases, but factors that govern strain abundance remain poorly defined. We show a commensal Escherichia coli and pathogenic Salmonella enterica serovar Typhimurium isolate both utilize nitrate for intestinal growth, each accesses this resource distinct biogeographical niche. Commensal E. utilizes epithelial-derived nitrate, whereas niche occupied by S. is derived from phagocytic infiltrates. Surprisingly, avirulent was...
Optogenetic protein dimerization systems are powerful tools to investigate the biochemical networks that cells use make decisions and coordinate their activities. These tools, including improved Light-Inducible Dimer (iLID) system, offer ability selectively recruit components subcellular locations, such as micron-scale regions of plasma membrane. In this way, role individual proteins within signaling can be examined with high spatiotemporal resolution. Currently, consistent recruitment is...
Abstract Synthetic lethality provides an attractive strategy for developing targeted cancer therapies. For example, cells with high levels of microsatellite instability (MSI-H) are dependent on the Werner (WRN) helicase survival. However, mechanisms that regulate WRN spatiotemporal dynamics remain poorly understood. Here, we used single-molecule tracking (SMT) in combination a inhibitor to examine within nuclei living cells. inhibition traps chromatin, requiring p97/VCP extraction and...
In the mammalian intestine, flagellar motility can provide microbes competitive advantage, but also threatens spatial segregation established by host at epithelial surface. Unlike microbicidal defensins, previous studies indicated that protective activities of human α-defensin 6 (HD6), a peptide secreted Paneth cells small resides in its remarkable ability to bind microbial surface proteins and self-assemble into fibers nets. Given flagellin, we proposed HD6 might be an effective inhibitor...
Abstract Synthetic lethality, the concept in which co-occurrence of two genetic events leads to cell death while either single event alone does not, is an attractive strategy for targeted cancer therapies. A recent example synthetic lethality as a therapeutic paradigm observation that cells with high levels microsatellite instability (MSI-H) are dependent on Werner (WRN) RecQ helicase survival. However, mechanisms regulate WRN spatiotemporal dynamics not fully understood. In this study, we...
Abstract Antimicrobial peptides are key effector molecules throughout nature responsible in part for the defense against microbial pathogens and influencing composition of colonizing microbes. Defensins major antimicrobial produced by Paneth cells mammalian small intestine essential maintaining homeostasis between host gut While other defensins have potent microbicidal activity, human cell alpha defensin 6 (HD6) lacks such yet provides protection from invasion enteric bacterial pathogens....
Abstract Optogenetic protein dimerization systems are powerful tools to investigate the biochemical networks that cells use make decisions and coordinate their activities. These tools, including improved Light-Inducible Dimer (iLID) system, offer ability selectively recruit components subcellular locations, such as micron-scale regions of plasma membrane. In this way, role individual proteins within signaling can be examined with high spatiotemporal resolution. Currently, consistent...
Abstract Antimicrobial peptides are key effector molecules throughout nature responsible in part for the defense against microbial pathogens and influencing composition of colonizing microbes. Defensins major antimicrobial produced by Paneth cells mammalian small intestine essential maintaining homeostasis between host microbe. While mature defensin typically have microbicidal activity, human alpha 6 (HD6) lacks such activity yet provides protection from invasion bacterial pathogens. Using a...