A5024555478- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Nanopore and Nanochannel Transport Studies
- Food composition and properties
- RNA Research and Splicing
- Genetic Mapping and Diversity in Plants and Animals
- Laser-Plasma Interactions and Diagnostics
- Genetics and Plant Breeding
- Adenosine and Purinergic Signaling
- GABA and Rice Research
- Fermentation and Sensory Analysis
- Hydrogels: synthesis, properties, applications
- Terahertz technology and applications
- Gyrotron and Vacuum Electronics Research
- Angiogenesis and VEGF in Cancer
- Seed and Plant Biochemistry
- Probiotics and Fermented Foods
- Laser-Matter Interactions and Applications
- Mesenchymal stem cell research
- Superconducting and THz Device Technology
- Pluripotent Stem Cells Research
- Advanced Materials and Mechanics
- Plant Stress Responses and Tolerance
- Neurogenesis and neuroplasticity mechanisms
Northwest Normal University
2015-2025
Hebei Academy of Agriculture and Forestry Sciences
2015-2024
Guangdong Academy of Agricultural Sciences
2023-2024
Nantong University
2021-2024
Ministry of Agriculture and Rural Affairs
2023-2024
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2008-2023
Hebrew University of Jerusalem
2023
Max Planck Institute for Marine Microbiology
2023
Jiaying University
2014-2022
Capital Medical University
2022
Hfq, a bacterial member of the Sm family RNA-binding proteins, is required for action many small regulatory RNAs that act by basepairing with target mRNAs. Hfq binds this efficiently. We have used co-immunoprecipitation and direct detection bound on genomic microarrays to identify members RNA family. This approach was extremely sensitive; even Hfq-binding expressed at low levels were readily detected. At least 15 46 known in E. coli interact Hfq. In addition, high signals other intergenic...
Exposure of E. coli to hydrogen peroxide induces the transcription a small RNA denoted oxyS. The oxyS is stable, abundant, and does not encode protein. activates represses expression numerous genes in coli, eight targets, including encoding transcriptional regulators FhlA σs, were identified. also leads reduction spontaneous chemically-induced mutagenesis. Our results suggest that acts as regulator integrates adaptation with other cellular stress responses helps protect cells against...
ABSTRACT While the model organism Escherichia coli has been subject of intense study for decades, full complement its RNAs is only now being examined. Here we describe a survey E. transcriptome carried out using differential RNA sequencing (dRNA-seq) approach, which can distinguish between primary and processed transcripts, an automated prediction algorithm transcriptional start sites (TSS). With criterion expression under at least one three growth conditions examined, predicted 14,868 TSS...
DsrA is an 85-nucleotide, untranslated RNA that has multiple regulatory activities at 30 degrees C. These include the translational regulation of RpoS and H-NS, global transcriptional regulators in Escherichia coli. Hfq E. coli protein necessary for vitro vivo replication phage Qbeta. also plays a role degradation numerous transcripts. Here we show hfq mutant strain defective DsrA-mediated both rpoS hns. The defect expression can be partially overcome by overexpression DsrA. does not...
In a previous bioinformatics-based search for novel small-RNA genes encoded by the Escherichia coli genome, we identified region, IS063, located between ompN and ydbK genes, that encodes an approximately 100-nucleotide transcript. Here show expression of this small RNA is increased at low temperature in minimal medium. Twenty-two nucleotides 5' end transcript have potential to form base pairs with leader sequence mRNA encoding outer membrane protein OmpC. The deletion IS063 ompC-luc...
Proteins of 50 or fewer amino acids are poorly characterized in all organisms. The corresponding genes challenging to reliably annotate, and it is difficult purify characterize the small protein products. Due these technical limitations, little known about abundance proteins, not mention their biological functions. To begin proteins Escherichia coli, we assayed accumulation under a variety growth conditions after exposure stress. We found that many accumulate specific stress induced. For...
Three-dimensional (3D) cells in vitro culture are becoming increasingly popular cancer research because some important signals lost when cultured a two-dimensional (2D) substrate. In this work, bacterial cellulose (BC)/gelatin hydrogels were successfully synthesized and investigated as scaffolds for to simulate tumor microenvironment. Their properties ability support normal growth of evaluated. particular, the human breast cell line (MDA-MD-231) was seeded into BC/gelatin investigate their...
Hfq, a bacterial RNA chaperone, stabilizes small regulatory RNAs (sRNAs) and facilitates sRNA base-pairing with target mRNAs. Hfq has conserved N-terminal domain poorly disordered C-terminal (CTD). In transcriptome-wide examination of the effects chromosomal CTD deletion (Hfq1-65), Escherichia coli mutant was most defective for accumulation sRNAs that bind proximal distal faces (Class II sRNAs), but other also were affected. There only modest on levels mRNAs, suggesting little disruption...
Journal Article Nucleotide sequence of a newly-identified Escherichia coli gene, stpA, encoding an H-NS-like protein Get access Aixia Zhang, Zhang Molecular Genetics Program, Wadsworth Center for Laboratories and Research, New York State Department Health School Public Health, University YorkP0 Box 509, Albany, NY 1201-0509, USA Search other works by this author on: Oxford Academic PubMed Google Scholar Marlene Belfort * *To whom correspondence should be addressed Nucleic Acids Volume 20,...
Summary The Escherichia coli tolC encodes a major outer membrane protein with multiple functions in export (e.g. diverse xenobiotics, haemolysin) and as an attachment site for phage colicins. is regulated part by MarA, SoxS Rob, three paralogous transcriptional activators which bind sequence called the marbox activate antibiotic superoxide resistance functions. Two previously identified promoters, p 1 2, are not or Rob but 2 activated EvgAS PhoPQ also regulate other Using fusions primer...
Jujube pulp separated from Ziziphus jujube is often discarded after processing, resulting in a serious waste of resources and environmental pollution. Herein, was used as raw material for vinegar fermentation. To investigate the dynamic distribution microorganisms flavor substances ZJV, correlations between variables (e.g., total acid, reducing sugar, temperature) (organic acids, amino volatile substances) were analyzed. Physicochemical indicators (temperature, alcohol) main factors...