A5089780460- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Mitochondrial Function and Pathology
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Biomedical and Engineering Education
- Metabolism and Genetic Disorders
- ATP Synthase and ATPases Research
- Mesenchymal stem cell research
- Cellular Mechanics and Interactions
- Angiogenesis and VEGF in Cancer
- Electrospun Nanofibers in Biomedical Applications
- Biomedical Ethics and Regulation
- Bone Tissue Engineering Materials
- Microfluidic and Bio-sensing Technologies
- Nanoparticle-Based Drug Delivery
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Silk-based biomaterials and applications
- Aortic Disease and Treatment Approaches
- Nanoplatforms for cancer theranostics
- Electromagnetic Fields and Biological Effects
- Aortic aneurysm repair treatments
- Cancer Research and Treatments
- Spine and Intervertebral Disc Pathology
University of Arkansas at Fayetteville
2017-2024
University of Toledo
2024
University of Jordan
2024
Centre for Biomedical Engineering and Physics
2024
Biomechanics Institute of Valencia
2024
Virginia Commonwealth University
2009-2021
George Washington University Hospital
2018-2019
University of Richmond
2014
University of Georgia
2002-2006
Abstract Research on the cell fate determination of embryonic stem cells is enormous interest given therapeutic potential in regenerative therapy. Human (hESCs) have ability to renew themselves and differentiate into all three germ layers. The main focus this study was examine factors affecting derivation further proliferation multipotent neuroepithelial (NEP) from hESCs. hESCs cultured serum-deprived defined medium developed distinct tube structures could be isolated either by dissociation...
Abstract Background The pluripotency and self renewing properties of human embryonic stem cells (hESC) make them a valuable tool in the fields developmental biology, pharmacology regenerative medicine. Therefore, there exists immense interest devising strategies for hESC propagation differentiation. Methods involving simulation native cell microenvironment, both chemical physical, have received lot attention recent years. Equally important is evidence that can also sense mechanical their...
The DNA double-strand break (DSB) is the most toxic form of damage. Studies aimed at characterizing repair during development suggest that homologous recombination (HRR) more critical in pluripotent cells compared to differentiated somatic which nonhomologous end joining (NHEJ) dominant. We have characterized damage response (DDR) and quality human embryonic stem (hESCs), vitro-derived neural cells. Resolution ionizing radiation-induced foci (IRIF) was used as a surrogate for DSB repair....
Low pH in the tumor micromilieu is a recognized pathological feature of cancer. This attribute cancerous cells has been targeted herein for controlled release chemotherapeutics at tumour site, while sparing healthy tissues. To this end, pH-sensitive, hollow ZnO-nanocarriers loaded with paclitaxel were synthesized and their efficacy studied breast cancer vitro vivo. The nanocarriers surface functionalized folate using click-chemistry to improve uptake by malignant that over-express...
Silk fibroin (SF) is a natural protein, which derived from the Bombyx mori silkworm. SF based porous materials are extensively investigated for biomedical applications, due to their biocompatibility and biodegradability. In this work, CO2 assisted acidification used synthesize hydrogels that subsequently converted aerogels. The aqueous silk concentration tune morphology textural properties of As increases 2 6 wt%, surface area resultant aerogels 260 308 m(2) g(-1) compressive modulus 19.5...
BACKGROUNDSignaling mechanisms involved in early human germ cell development are largely unknown and believed to be similar mouse development; however, there may species specific differences. KIT ligand (KITL) Bone morphogenetic protein 4 (BMP4) necessary play an important role development.
The Covid-19 pandemic caused a sudden shift towards remote learning, moving classes to online formats. Not exempt from this switch, laboratory courses traditionally taught in-person were also moved methods, costing students the opportunity learn these skills hands-on. In order for instructors provide course materials effectively and engagingly, non-traditional methods should be explored. Virtual reality (VR) has become more accessible in recent years. VR simulations have been used many years...
Abstract Even though astrocytes are critical for both normal brain functions and the development progression of neuropathological states, including neuroinflammation associated with neurodegenerative diseases, mechanisms controlling gene expression during astrocyte differentiation poorly understood. Thus far, several signaling pathways were shown to regulate differentiation, JAK-STAT, bone morphogenic protein-2/Smads, Notch. More recently, a family nuclear factor-1 (NFI-A, -B, -C, -X) was...
Many incurable mitochondrial disorders result from mutant DNA (mtDNA) and impaired respiration. Leigh's syndrome (LS) is a fatal neurodegenerative disorder of infants, Leber's hereditary optic neuropathy (LHON) causes blindness in young adults. Treatment LHON LS cells harboring G11778A T8993G mtDNA, respectively, by >90%, with healthy donor mtDNA complexed recombinant human transcription factor A (rhTFAM), improved respiration ∼1.2-fold restored >50% ATP synthase function cells....
The therapeutic index of poorly water-soluble drugs is often hampered due to poor pharmacokinetics, reduced blood retention, and lack effective drug concentrations in the tumor region. In order overcome these issues, are delivered by use delivery vehicles provide an enhanced index. Gold nanoparticles synthesized micellar networks amphiphilic block copolymer (AuNM) efficient nanocarrier for tissue- site-specific owing their low cytotoxicity immunogenicity. AuNM formed exploiting properties...
Abstract Human embryonic stem cells (ESCs) have generated enormous interest due to their ability self‐renew and produce many different cell types. In conjunction with microarray technology, human ESCs provide a powerful tool for employing systems‐based approach deciphering the molecular mechanisms that control pluripotency early development. Recent work has focused on defining “stemness” based experimental analytical approaches in both mouse ESCs. Using mixed linear model statistical...
Abdominal aortic aneurysms (AAAs) involve slow proteolysis and loss of structural matrix components (collagen elastin), which lead to wall thinning, weakening ultimate rupture. At this time, no established non-surgical therapy is available or arrest AAA growth. Inhibiting metalloproteases (MMPs; e.g. MMP2 −9) overexpressed within AAAs insufficient growth, since resident smooth muscle cells (SMCs) are poorly elastogenic cannot overcome elastolysis reinstate a healthy elastic matrix. Towards...
Human embryonic stem cells (hESCs) and their differentiated progeny allow for investigation of important changes/events during normal development. Currently most the research is focused on proteinacous changes occurring as a result differentiation little known about in cell surface glycosylation patterns. Identification lineage specific glycans can help understanding role maintenance, proliferation differentiation. Furthermore, these serve markers isolation homogenous populations cells....
Introduction Immersive virtual reality (VR) based laboratory demonstrations have been gaining traction in STEM education as they can provide hands-on experience. VR also facilitate experiential and visual learning enhanced retention. However, several optimizations of the implementation, in-depth analyses advantages trade-offs technology, assessment receptivity modern techniques are required to ensure better utilization VR-based labs. Methods In this study, we developed for a biomolecular...
Single drug therapies for cancer are often suboptimal and may not provide long term clinical benefits. To overcome this obstacle effective treatment the applications of two or more drugs preferable. A limitation multidrug use is varying pharmacokinetics different drugs. these impediments, we designed synthesized multi-layered polyvinyl alcohol tethered hollow manganese ferrite nanocarriers capable encapsulating with unique attributes sensitivity towards tumor acidic milieu, mono-dispersive,...