A5008175087- Fatty Acid Research and Health
- Eicosanoids and Hypertension Pharmacology
- MicroRNA in disease regulation
- Bone health and treatments
- Inflammatory mediators and NSAID effects
- Circular RNAs in diseases
- Microbial metabolism and enzyme function
- Peroxisome Proliferator-Activated Receptors
- Cholesterol and Lipid Metabolism
- Bone Metabolism and Diseases
- Heterotopic Ossification and Related Conditions
- Antiplatelet Therapy and Cardiovascular Diseases
- Metabolomics and Mass Spectrometry Studies
- Metabolism and Genetic Disorders
- Lipid metabolism and biosynthesis
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Radiopharmaceutical Chemistry and Applications
- Diet and metabolism studies
- Cancer, Hypoxia, and Metabolism
- Alcohol Consumption and Health Effects
- Connective tissue disorders research
- Pharmacology and Obesity Treatment
- Cell Adhesion Molecules Research
- Fibroblast Growth Factor Research
Inserm
2014-2024
Université Claude Bernard Lyon 1
2014-2024
Pathophysiology, Diagnosis and Treatment of Bone Diseases
2011-2024
United States Congress
2022
Hôpital Edouard Herriot
2018-2020
Hospices Civils de Lyon
2018
Università Campus Bio-Medico
2013
Institut Curie
2013
Institut National de Recherche en Santé Publique
2011
Université de Lyon
2009
Unsaturated lysophosphatidylcholines (lysoPtdCho) bound to albumin circulate in blood plasma and seem be a novel transport system for carrying polyunsaturated fatty acids (PUFA) tissues that are rich these acids, such as the brain. The potential of lysoPtdCho significant source PUFA cells has been assessed by comparing their concentration with unsaturated non-esterified (NEFA) albumin. In humans, was 25.9+/-3.1 nmol/ml lysoPtdCho, compared 33.4+/-9.6 NEFA; rats equivalent values 14.2+/-0.6...
miRNAs are master regulators of gene expression that play key roles in cancer metastasis. During bone metastasis, metastatic tumor cells must rewire their biology and express genes normally expressed by (a process called osteomimicry), which endow with full competence for outgrowth the marrow. Here, we establish miR-30 family members miR-30a, miR-30b, miR-30c, miR-30d, miR-30e as suppressors breast metastasis regulate multiple pathways, including osteomimicry. Low primary tumors from...
Bone metastasis remains a major cause of mortality and morbidity in breast cancer. Therefore, there is an urgent need to better select high-risk patients order adapt patient's treatment prevent bone recurrence. Here, we found that integrin alpha5 (ITGA5) was highly expressed metastases, compared lung, liver, or brain metastases. High ITGA5 expression primary tumors correlated with the presence disseminated tumor cells marrow aspirates from early stage cancer (n = 268; p 0.039). also...
At variance with the current view that only liver and kidney are gluconeogenic organs, because both tissues to express glucose-6-phosphatase (Glc6Pase), we have recently demonstrated Glc6Pase gene is expressed in small intestine rats humans it induced insulinopenic states such as fasting diabetes. We used a combination of arteriovenous balance isotopic techniques, reverse transcription–polymerase chain reaction, Northern blot analysis, enzymatic activity assays. report rat can release...
The transcription factor TWIST1 induces epithelial-mesenchymal transition and/or escape to the oncogenic-induced failsafe program, facilitating intravasation of breast cancer cells in systemic circulation and their dissemination lungs. Its involvement bone metastasis is unknown. To address this question, human osteotropic MDA-MB-231/B02 were stably transfected with a Tet-inducible vector encoding for TWIST1, whose expression was specifically repressed presence doxycycline (dox)....
Lysyl oxidase (LOX) is a secreted copper-dependent amine whose primary function to drive collagen crosslinking and extracellular matrix stiffness. LOX in colorectal cancer synergizes with hypoxia-inducible factor-1 (HIF-1) promote tumor progression. Here we investigated whether LOX/HIF1 endows cells full competence for aggressive colonization bone. We show that high expression tumors from patients was associated poor clinical outcome, irrespective of HIF-1 In addition, expressed by the bone...
We studied in rats the expression of genes involved gluconeogenesis from glutamine and glycerol small intestine (SI) during fasting diabetes. From Northern blot enzymatic studies, we report that only phosphoenolpyruvate carboxykinase (PEPCK) activity is induced at 24 h fasting, whereas glucose-6-phosphatase (G-6-Pase) 48 h. Both then plateau, glutaminase glycerokinase strikingly rebound between 72 The two latter are fully expressed streptozotocin-diabetic rats. arteriovenous balance isotopic...
Docosahexaenoic acid (22:6) decreases blood platelet function and is highly concentrated in the brain where its depletion leads to functional impairments. Because platelets barrier capillary endothelium cannot hydrolyze complex lipids for fatty (FA) uptake, nonesterified FA (NEFA) bound albumin are assumed be delivery route of these cells. The supply 13C-labeled 22:6 cells by plasma was studied humans after a single ingestion this esterified triglyceride (TG). 13C/12C ratio, measured gas...
In the context of scarcity biomarkers for knee osteoarthritis (OA), we examined associations prevalent and incident OA with expression levels serum miRNAs in subjects without OA.With a next-generation sequencing approach, compared miRome 10 women age-matched healthy subjects. By real-time qPCR, analyzed 19 at baseline selecting 43 (Kellgren Lawrence score 2/3), 23 over 4-year follow-up 67 or matched age body mass index.Serum miR-146a-5p was significantly increased group controls (relative...
PEPCK is a key enzyme of gluconeogenesis in liver and kidney. Recently, we have shown that small intestine also contributes to the endogenous glucose production insulinopenia rats glutamine main precursor synthesized this tissue. The expression gene rat human effect streptozotocin-induced diabetes fasting been studied using reverse transcriptase-polymerase chain reaction, Northern blot analysis, determination activity. expressed along whole adult human. abundance mRNA was increased...
Human platelet lipids were enriched in vitro with different amounts of either docosahexaenoic acid (22:6n-3), eicosapentaenoic (20:5n-3) or linoleic (18:2n-6). Of the total fatty incorporated, between 82 and 95% was associated phospholipid (PL) fraction, remainder as neutral lipid hydroxy acid. Within PL majority (64% total) each incorporated into phosphatidylcholine. It found that aggregation induced by thromboxane A2/prostaglandin H2 mimetic...
Washed human platelets were pre-loaded with icosapentaenoic acid (EPA), docosahexaenoic (DHA) or EPA + DHA and tested for their aggregation response in comparison control platelets. In fatty acid-rich platelets, an inhibition of the could be observed when induced by thrombin, collagen U-46619. The strongest was DHA-rich it reduced incorporated presence EPA. Study distribution cell lipids after loading showed that around 90% taken up acylated into phospholipids a very small amount (less than...
Lipoxygenase activity was measured in human platelet subcellular fractions. From a sonicated preparation, granule fraction, mixed membranes (surface and intracellular) cytosol fractions were separated by differential centrifugation. With respect to activities the lipoxygenase slightly enriched both mixed-membrane consistently de-enriched Approx. 65% 20% of total cell enzyme found respectively, with only 8% present fraction. Additionally we purified surface- intracellular-membrane...
Unsaturated lysophosphatidylcholines (lysoPtdCho) bound to albumin circulate in blood plasma and seem be a novel transport system for carrying polyunsaturated fatty acids (PUFA) tissues that are rich these acids, such as the brain. The potential of lysoPtdCho significant source PUFA cells has been assessed by comparing their concentration with unsaturated non-esterified (NEFA) albumin. In humans, was 25.9±3.1 nmol/ml lysoPtdCho, compared 33.4±9.6 NEFA; rats equivalent values 14.2±0.6...