A5020447126- Gut microbiota and health
- Inflammatory Bowel Disease
- IL-33, ST2, and ILC Pathways
- Microscopic Colitis
- Probiotics and Fermented Foods
- Clostridium difficile and Clostridium perfringens research
- Gastrointestinal motility and disorders
- Cancer Cells and Metastasis
- Mycobacterium research and diagnosis
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Microbial Metabolites in Food Biotechnology
- Nutritional Studies and Diet
- Diet and metabolism studies
- Digestive system and related health
- Immunodeficiency and Autoimmune Disorders
- Autoimmune and Inflammatory Disorders Research
- Eosinophilic Esophagitis
- Infant Nutrition and Health
- Glycosylation and Glycoproteins Research
- Antimicrobial Peptides and Activities
- Neutropenia and Cancer Infections
- Connexins and lens biology
- Colorectal Cancer Surgical Treatments
- Genetic factors in colorectal cancer
Case Western Reserve University
2017-2025
University School
2017-2025
University College Cork
2012-2021
APC Microbiome Institute
2021
University Hospitals of Cleveland
2020
National University of Ireland
2012-2019
Technical University of Munich
2015-2017
Weihenstephan-Triesdorf University of Applied Sciences
2017
In this study, we tested the hypothesis that milk oligosaccharides may contribute not only to selective growth of bifidobacteria, but also their specific adhesive ability. Human (3′sialyllactose and 6′sialyllactose) a commercial prebiotic (Beneo Orafti P95; oligofructose) were assayed for ability promote adhesion Bifidobacterium longum subsp. infantis ATCC 15697 HT-29 Caco-2 human intestinal cells. Treatment with or 3′sialyllactose did enhance adhesion. However, treatment 6′sialyllactose...
There are complex interactions between aging, frailty, diet, and the gut microbiota; modulation of microbiota by diet could lead to healthier aging. The purpose this study was test effect diets differing in sugar, fat, fiber content upon mice humanized with from healthy or frail older people. We also performed a 6-month dietary supplementation three human cohorts representing distinct life-stages.Mice were colonized then underwent an 8-week intervention either high-fiber/low-fat typical...
Enhanced barrier function is one mechanism whereby commensals and probiotic bacteria limit translocation of foreign antigens or pathogens in the gut. However, protection not exhibited by all strain-specific molecules involved remain to be clarified. We evaluated effects 33 individual Lactobacillus salivarius strains on hydrogen peroxide (H(2)O(2))-induced impairment human epithelial Caco-2 cells. These showed markedly different H(2)O(2)-induced reduction transepithelial resistance (TER). The...
Significance Here we demonstrate that IL-1α neutralization reduces the severity of disease in a mouse model Crohn’s disease. Unexpectedly, exerts its antiinflammatory effects by modulating gut microbial ecosystem, and corrected mucosal dysbiosis. Although studies report microbiome affects cytokine production, few have shown specific pivotal such as alters composition function microbiome. The study demonstrates predictive relationship between abundance bacterial species. findings...
ABSTRACT Lactobacillus salivarius strain UCC118 is a human intestinal isolate that has been extensively studied for its potential probiotic effects in and animal models. The objective of this study was to determine the effect L. on gene expression responses Caco-2 cell line improve understanding how might modulate epithelial phenotypes. Exposure cells led induction several genes ( TNFAIP3 , NFKBIA BIRC3 ) are negative regulators inflammatory signaling pathways. Induction chemokines (CCL20,...
Death receptor 3 (DR3) and its ligand, tumor necrosis factor-like ligand 1A (TL1A), regulates the balance between effector regulatory T cells in IBD. While IL-9-secreting Th9 are linked to ulcerative colitis, their role Crohn's disease (CD) is unclear. We investigated of DR3 signaling cell differentiation mouse models CD-like ileitis colitis. Polarized with functional DR3-deficient from SAMP WT (Th9WT) DR3-/-×SAMP (Th9KO) mice, respectively, were characterized adoptively transferred into...
We previously showed that abundance of Candida tropicalis is significantly greater in Crohn's disease patients compared with first-degree relatives without disease. The aim this study was to determine the effects and mechanisms action C infection on intestinal inflammation injury mice.C57BL/6 mice were inoculated tropicalis, colitis induced by administration dextran sodium sulfate drinking water. Disease severity permeability subsequently evaluated endoscopy, histology, quantitative...
Abstract The mechanisms through which cells of the host innate immune system distinguish commensal bacteria from pathogens are currently unclear. Toll-like receptors (TLRs) a class pattern recognition (PRRs) expressed by recognize microbe-associated molecular patterns (MAMPs) common to both and pathogenic bacteria. Of different TLRs, TLR2/6 bacterial lipopeptides trigger cytokines responses, especially Gram-positive Gram-negative pathogens. We report here that TLR2 is dispensable for...
Death receptor 3 (DR3), a member of the tumor necrosis factor (TNFR) superfamily, has been implicated in regulating T-helper type-1 (TH1), type-2 (TH2), and type-17 (TH17) responses as well regulatory T cell (Treg) innate lymphoid (ILC) functions during immune-mediated diseases. However, role DR3 controlling lymphocyte inflammatory bowel disease (IBD) is not fully understood. Recent studies have shown that activation signaling modulates Treg expansion suggesting stimulation represents...
Abstract Background Inflammatory bowel disease (IBD) is a lifelong digestive characterized by periods of severe inflammation and remission. To our knowledge, this the first study showing variable effect on ileitis severity from human gut microbiota isolated IBD donors in remission that healthy controls mouse model IBD. Methods We conducted series single-donor intensive nonintensive fecal transplantation (FMT) experiments using feces patients non-IBD (N = 9 donors) Crohn’s (CD)-like develops...
Objective: Death receptor 3 (DR3) and its ligand tumor necrosis factor like 1A (TL1A), are involved in the regulation of balance between effector regulatory T cells IBD. New evidence suggests a role IL-9-secreting Th9 pathogenesis ulcerative colitis (UC), although molecular pathways through which IL-9 may mediate intestinal inflammation Crohn's disease (CD) still unclear. Design: We investigated DR3 signaling differentiation mouse models CD-like ileitis colitis, including SAMP1/YitFc (SAMP)...
Gut homeostasis involves interrelated biological networks that include the immune system, specialized cells of epithelium, such as Paneth and goblet cells, well triggers derived from microbiota. Disruption these homeostatic interactions may lead to pathogenesis inflammatory bowel diseases (IBD). To develop more targeted individual treatments in Crohn's disease ulcerative colitis, it becomes important link key mechanisms distinct IBD subsets. For first time, our laboratory demonstrated a...
TNF-like cytokine 1A (TL1A) and its functional receptor, death-domain-receptor-3 (DR3), are multifunctional mediators of effector regulatory immunity. We aimed to evaluate the role therapeutic potential TL1A/DR3 signaling in Crohn's disease–like ileitis. Ileitis-prone SAMP1/YitFc (SAMP) TNFΔARE/+ mice were rendered deficient for DR3 or TL1A by microsatellite marker-assisted backcrossing. Pathological immunological characteristics compared between control knockout mice, mucosal...
Intestinal epithelial barrier dysfunction is a risk factor in the pathogenesis of Crohn's disease (CD); however, no corrective FDA-approved therapies exist. We used an enteroid (EnO)-based system two murine models experimental CD, SAMP1/YitFc (SAMP) and TNFΔARE/+ (TNF). While severely inflamed SAMP mice do not generate EnOs, "inflammation-free" form EnO structures with impaired morphology reduced intestinal stem cell (ISC) Paneth viability. validated these findings TNF concluding that...