A5070485730- Gut microbiota and health
- Inflammatory Bowel Disease
- Clostridium difficile and Clostridium perfringens research
- Microscopic Colitis
- Viral gastroenteritis research and epidemiology
- Helicobacter pylori-related gastroenterology studies
- IL-33, ST2, and ILC Pathways
- Gastrointestinal motility and disorders
- Probiotics and Fermented Foods
- Digestive system and related health
- Diet and metabolism studies
- Eosinophilic Esophagitis
- Infection Control and Ventilation
- Drug Transport and Resistance Mechanisms
- Nutrition, Genetics, and Disease
- Diverticular Disease and Complications
- Mycobacterium research and diagnosis
- Genetic factors in colorectal cancer
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Diet, Metabolism, and Disease
- Fatty Acid Research and Health
- Animal testing and alternatives
- Coconut Research and Applications
- Bacterial Identification and Susceptibility Testing
Case Western Reserve University
2016-2025
University School
2016-2025
University Hospitals of Cleveland
2018-2024
Campus Bio Medico University Hospital
2021
RELX Group (Netherlands)
2020
Digestive Health Research Institute
2019
Center for Digestive and Liver Diseases
2018
University Hospitals Cleveland Medical Center
2015
Indiana University School of Medicine
2015
The Ohio State University
2010-2014
Epidemiological studies indicate that the use of artificial sweeteners doubles risk for Crohn's disease (CD). Herein, we experimentally quantified impact 6-week supplementation with a commercial sweetener (Splenda; ingredients sucralose maltodextrin, 1:99, w/w) on both severity CD-like ileitis and intestinal microbiome alterations using SAMP1/YitFc (SAMP) mice.Metagenomic shotgun DNA sequencing was first used to characterize ileitis-prone SAMP mice. Then, 16S rRNA sequencing, quantitative...
Abstract Clostridium difficile was isolated from 12 (20%) of 60 retail ground meat samples purchased over a 10-month period in 2005 Canada. Eleven isolates were toxigenic, and 8 (67%) classified as toxinotype III. The human health implications this finding are unclear, but with the virulence III strains further studies required.
We investigated Clostridium difficile in calves and the similarity between bovine human C. PCR ribotypes by conducting a case-control study of from 102 dairy farms Canada. Fecal samples 144 with diarrhea 134 control were cultured for tested an ELISA toxins A B. was isolated 31 278 calves: 11 (7.6%) 20 (14.9%) controls (p = 0.009). Toxins detected calf feces 58 (56.8%) farms, 57 (39.6%) diarrhea, 28 (20.9%) 0.0002). ribotyping isolates showed 8 distinct patterns; 7 have been identified...
ABSTRACT Clostridium difficile spores can survive extended heating at 71°C (160°F), a minimum temperature commonly recommended for adequate cooking of meats. To determine the extent to which higher temperatures would be more effective killing C. , we quantified ( D values) effect moist heat 85°C (145°F, 0 30 min) on and compared it effects 71 63°C. Fresh (1-week-old) aged (≥20-week-old) from food animals were tested in multiple experiments. Heating markedly reduced spore recovery all...
We previously reported Clostridium difficile in 20% of retail meat Canada, which raised concerns about potential foodborne transmissibility. Here, we studied the genetic diversity C. meats, using a broad Canadian sampling infrastructure and 3 culture methods. found 6.1% prevalence indications possible seasonality (highest winter).
Abstract Histology is fundamental to assess two-dimensional intestinal inflammation; however, inflammatory bowel diseases (IBDs) are often indistinguishable microscopically on the basis of mucosal biopsies. Here, we use stereomicroscopy (SM) rapidly profile entire topography and inflammation. We examine surface >700 mice (encompassing >16 strains various IBD-models), create a profiling catalogue 3D-stereomicroscopic abnormalities demonstrate that with comparable histological scores...
The main form of COVID-19 transmission is via "oral-respiratory droplet contamination" (droplet: very small drop liquid) produced when individuals talk, sneeze, or cough. In hospitals, health-care workers wear facemasks as a minimum medical "
Significance Here we demonstrate that IL-1α neutralization reduces the severity of disease in a mouse model Crohn’s disease. Unexpectedly, exerts its antiinflammatory effects by modulating gut microbial ecosystem, and corrected mucosal dysbiosis. Although studies report microbiome affects cytokine production, few have shown specific pivotal such as alters composition function microbiome. The study demonstrates predictive relationship between abundance bacterial species. findings...
Innate lymphoid cells (ILCs) are enriched at barrier surfaces, including the gastrointestinal tract. While most studies have focused on balance between pathogenic group 1 ILCs (ILC1s) and protective ILC3s in maintaining gut homeostasis during chronic intestinal inflammation, such as Crohn's disease (CD), less is known regarding ILC2s. Using an established murine model of CD-like ileitis, i.e., SAMP1/YitFc (SAMP) mouse strain, we showed that ILC2s, compared with ILC1s ILC3s, were increased...
ABSTRACT Domestic cats have several features that make them ideal vehicles for interspecies transmission of influenza viruses; however, they been largely overlooked as potential reservoirs or bridging hosts. In this study, we conducted serological surveillance to assess the prevalence novel pandemic H1N1 well seasonal human virus infections in domestic Ohio. Four hundred serum samples collected from (September 2009 September 2010) were tested using a hemagglutination inhibition (HI) test....
Germ-Free (GF) research has required highly technical pressurized HEPA-ventilation anchored systems for decades. Herein, we validated a GF system that can be easily implemented and portable using Nested Isolation (NesTiso). GF-standards achieved housing mice in non-HEPA-static cages, which only need to nested 'one-cage-inside-another' resembling 'Russian dolls'. After 2 years of monitoring ~100,000 GF-mouse-days, NesTiso showed maintained life (>1.3 years), with low animal...