A5017367462- Virus-based gene therapy research
- Immunodeficiency and Autoimmune Disorders
- Blood disorders and treatments
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Adenosine and Purinergic Signaling
- Antiplatelet Therapy and Cardiovascular Diseases
- Renal and related cancers
- Hematological disorders and diagnostics
- Inflammatory mediators and NSAID effects
- RNA Interference and Gene Delivery
- Biotechnology and Related Fields
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Neuroblastoma Research and Treatments
- Orthopedic Infections and Treatments
- Cancer Research and Treatments
- Nanoplatforms for cancer theranostics
- S100 Proteins and Annexins
- Synthesis of β-Lactam Compounds
- Transplantation: Methods and Outcomes
- Dermatological and COVID-19 studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pluripotent Stem Cells Research
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2022-2024
The San Raffaele Telethon Institute for Gene Therapy
2022-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2022-2024
Hospital Universitario Fundación Jiménez Díaz
2016-2022
Centro de Investigación Biomédica en Red
2020-2022
Centre for Biomedical Network Research on Rare Diseases
2016-2022
Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas
2016-2022
Universidad Autónoma de Madrid
2016-2022
Instituto de Salud Carlos III
2017-2022
The Sleeping Beauty (SB) transposon system is a non-viral gene delivery platform that combines simplicity, inexpensive manufacture, and favorable safety features in the context of human applications. However, efficient correction hematopoietic stem progenitor cells (HSPCs) with vector systems, including SB, demands further refinement techniques. We set out to improve SB transfer into hard-to-transfect CD34+ by vectorizing components form minicircles are devoid plasmid backbone sequences are,...
In vivo detection and quantification of inflammation is a major goal in molecular imaging. Furthermore, cell-specific would be tremendous advantage the characterization many diseases. Here, we show how this can achieved through synergistic combination nanotechnology nuclear One most remarkable features hybrid approach possibility to tailor pharmacokinetics nanomaterial-incorporated biomolecule radionuclide. A good example covalent binding large amount neutrophil-specific, hydrophobic peptide...
Leukocyte adhesion deficiency type I (LAD-I) is a primary immunodeficiency caused by mutations in the ITGB2 gene and characterized recurrent life-threatening bacterial infections. These lead to defective or absent expression of β2 integrins on leukocyte surface, compromising extravasation at sites infection. Three different lentiviral vectors (LVs) conferring ubiquitous preferential CD18 myeloid cells were constructed tested human mouse LAD-I cells. All three hCD18-LVs restored CD11a...
CD18 is the common beta subunit of β2 integrins, which are expressed on hematopoietic cells. integrins essential for cell adhesion and leukocyte trafficking. Here we have analyzed expression in different subsets human stem progenitor cells (HSPCs) from cord blood (CB), bone marrow (BM), mobilized peripheral (mPB) samples. CD34+ were classified into CD18high CD18low/neg, each these populations was HSPC markers, as well their clonogenity, quiescence state, repopulating ability immunodeficient...
Transduction of hematopoietic stem and progenitor cells (HSPCs) with lentiviral vectors (LVs) constitutes a new therapeutic option for the treatment various monogenic diseases affecting lymphohematopoietic system. The development detailed preclinical studies gene therapy in animal disease models an essential step expanding application wide variety inherited acquired diseases. Here we describe efficient protocol to transduce HSPCs from wild-type Fanconi anemia mice either gene-marking or LVs....
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Intensive chemotherapy with autologous stem cell transplantation is a therapeutic tool used in paediatric oncology. In adult patients, peripheral blood CD34+ count superior to 20/μL enables an adequate collection of cells. There are no recommendations for children. This study aimed determine whether the circulating cells on day before cytapheresis predicts successful patients.We retrospectively studied all patients who underwent apheresis autotransplantation CHU Rennes between 2010 and 2019....