A5031474036- Cancer Research and Treatments
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Drug Transport and Resistance Mechanisms
- Cancer Cells and Metastasis
- CAR-T cell therapy research
- Cancer therapeutics and mechanisms
- Cancer Genomics and Diagnostics
- S100 Proteins and Annexins
- Cancer Mechanisms and Therapy
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- Cancer, Hypoxia, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Colorectal Cancer Treatments and Studies
- Barrier Structure and Function Studies
- Advanced biosensing and bioanalysis techniques
- 3D Printing in Biomedical Research
- Liver physiology and pathology
- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
- Wastewater Treatment and Nitrogen Removal
Max Delbrück Center
2016-2025
Charité - Universitätsmedizin Berlin
2015-2024
BioTez (Germany)
2024
Orthopädische Universitätsklinik
2023
German Cancer Research Center
2019-2023
Heidelberg University
2021-2023
Freie Universität Berlin
1994-2022
Humboldt-Universität zu Berlin
1999-2022
Deutschen Konsortium für Translationale Krebsforschung
2019-2022
Helios Hospital Berlin-Buch
2018-2022
BackgroundMetastasis formation in colon cancer severely reduces the survival rate patients. S100A4, a calcium-binding protein, is implicated promoting metastasis cancer.
Colorectal cancer (CRC) is the second most common cause of all deaths in Europe and Western world with a lifetime risk approximately 5%. Despite several improvements treatment patients unresectable CRC prognosis poor there need developing new strategies for metastatic chemorefractory disease. The S100 calcium binding protein A4 (S100A4) predicts metastasis formation reduced patient survival. S100A4 was previously identified as transcriptional target Wnt/β-catenin signaling pathway. Food Drug...
Abstract The immunomodulatory drugs (IMiDs) lenalidomide and pomalidomide are highly effective treatments for multiple myeloma. However, virtually all patients eventually relapse due to acquired drug resistance with resistance-causing genetic alterations being found only in a small subset of cases. To identify non-genetic mechanisms resistance, we here perform integrated global quantitative tandem mass tag (TMT)-based proteomic phosphoproteomic analyses RNA sequencing five paired...
Genotoxic stress leads to nuclear translocation of the Y-box transcription factor YB-1 and enhanced expression multidrug resistance gene MDR1. Because hyperthermia is used for treatment colon cancer in combination with chemoradiotherapy, we investigated influence on activity resistance-related genes. Here report that causes from cytoplasm into nucleus human carcinoma cells HCT15 HCT116. Nuclear was associated increased MDR1 MRP1 activity, which reflected strong efflux pump activity. However,...
The calcium-binding protein S100A4 is a central mediator of metastasis formation in colon cancer. target gene the Wnt/β-catenin pathway, which constitutively active majority cancers. In this study high-throughput screen was performed to identify small-molecule compounds targeting S100A4-promoter activity. calcimycin identified as transcriptional inhibitor S100A4. cancer cells treatment reduced mRNA and expression dose- time-dependent manner. S100A4-induced cellular processes associated with...
Colon cancer metastasis is often associated with activation of the Wnt/β-catenin signaling pathway and high expression mediator S100A4. We previously demonstrated transcriptional regulation S100A4 by β-catenin importance interconnection these cellular programs for metastasis. Here we probe hypothesis that nonsteroidal anti-inflammatory drug sulindac sulfide can inhibit colon intervening in thereby interdicting treated cell lines heterozygous gain-of-function wild-type sulindac. analyzed...
Transcriptional signatures are an indispensible source of correlative information on disease-related molecular alterations a genome-wide level. Numerous candidate genes involved in disease and factors predictive, as well prognostic, value have been deduced from such portraits, e.g. cancer. However, mechanistic insights into the regulatory principles governing global transcriptional changes lagging behind extensive compilations deregulated genes. To identify regulators transcriptome...
MACC1 (Metastasis Associated in Colon Cancer 1) is a key driver and prognostic biomarker for cancer progression metastasis large variety of solid tumor types, particularly colorectal (CRC). However, no inhibitors have been identified yet. Therefore, we aimed to target expression using luciferase reporter-based high-throughput screening with the ChemBioNet library more than 30,000 compounds. The small molecules lovastatin rottlerin emerged as most potent transcriptional inhibitors. They...
Bacterial toxins have evolved to an effective therapeutic option for cancer therapy. The Clostridium perfringens enterotoxin (CPE) is a pore-forming toxin with selective cytotoxicity. transmembrane tight junction proteins claudin-3 and -4 are known high affinity CPE receptors. Their expression highly upregulated in human cancers, including breast, ovarian colon carcinoma. binding claudins triggers membrane pore complex formation, which leads rapid cell death. Previous studies demonstrated...
The Sleeping Beauty (SB) transposon system is a non-viral gene delivery platform that combines simplicity, inexpensive manufacture, and favorable safety features in the context of human applications. However, efficient correction hematopoietic stem progenitor cells (HSPCs) with vector systems, including SB, demands further refinement techniques. We set out to improve SB transfer into hard-to-transfect CD34+ by vectorizing components form minicircles are devoid plasmid backbone sequences are,...
Here, we report on a fluorescent 1,2,4-thiadiazole derivative (oxidized form) and its reduced form, the corresponding iminoyl thiourea. The thiadiazole displays strong modulation of fluorescence behavior, selectively upon addition Cu(II), while thiourea functions as chemodosimeter for Hg(II). Additionally, Cu(II)-thiadiazole complex is characterized by HRMS, Hg(II)-induced desulfurization monitored mass spectrometry.
Abstract. We investigated the dynamics of denitrification and nitrous oxide (N2O) accumulation in 4 nitrate (NO−3) contaminated denitrifying sand gravel aquifers northern Germany (Fuhrberg, Sulingen, Thülsfelde Göttingen) to quantify their potential N2O emission evaluate existing concepts factors. Excess N2 – produced by was determined using argon (Ar) concentration groundwater as a natural inert tracer, assuming that this noble gas functions stable component does not change during...
Clostridium perfringens enterotoxin (CPE) causes food poisoning and antibiotic-associated diarrhea. It uses some claudin tight junction proteins (eg, claudin-4) as receptors to form Ca2+-permeable pores in the membrane, damaging epithelial cells small intestine colon. We demonstrate that only a subpopulation of colonic enterocytes which are characterized by apical dislocation claudins CPE-susceptible. CPE-mediated damage was enhanced if paracellular barrier impaired Ca2+ depletion,...