A5001827877- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Monoclonal and Polyclonal Antibodies Research
- Malaria Research and Control
- Salmonella and Campylobacter epidemiology
- Liver Disease Diagnosis and Treatment
- Vector-borne infectious diseases
- Viral gastroenteritis research and epidemiology
- Hepatitis C virus research
- Aquaculture disease management and microbiota
- Gastrointestinal Tumor Research and Treatment
- Protein purification and stability
- Cell Adhesion Molecules Research
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Escherichia coli research studies
- Infant Nutrition and Health
- Advanced Biosensing Techniques and Applications
- Metastasis and carcinoma case studies
- Virology and Viral Diseases
- Virus-based gene therapy research
- Animal Genetics and Reproduction
- Microbial infections and disease research
- Animal Disease Management and Epidemiology
- Biotin and Related Studies
Sekisui Medical (Japan)
2017-2022
National Agriculture and Food Research Organization
2010-2019
National Institute of Animal Health
2010-2015
Hokkaido University
2010
Flavivirus-infected sera are known to show cross-reactions in serodiagnoses of heterologous flavivirus infections. Japanese encephalitis virus (JEV) is endemic Asia and, Japan, many horses vaccinated against JEV. However, the cross-reactivity level JEV-vaccinated horse serodiagnosis West Nile (WNV) has not been clarified. The antibody WNV serological tests, such as plaque reduction neutralization test (PRNT), IgG indirect ELISA (IgG-ELISA) and hemagglutination inhibition (HI) test, was...
Erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, is a facultative intracellular Gram-positive bacterium. It has been shown that animals immunized with filtrate from E. rhusiopathiae cultures are protected against lethal challenge. In this study, we identified and characterized extracellular proteins to search for novel vaccine antigens. A concentrated culture supernatant Fujisawa strain, which found induce protection in mice, was analyzed using two-dimensional...
Recent increases in the number of patients with non-alcoholic steatohepatitis (NASH) warrant identification biomarkers for early detection hepatocellular carcinoma (HCC) associated NASH (NASH-HCC). IgM-free apoptosis inhibitor macrophage (AIM), which generally associates IgM blood and exerts its biological function by dissociation from IgM, may serve as an effective biomarker NASH-HCC. Here, we established a fully automatic high-throughput electrochemiluminescence immunoassay (ECLIA) to...
ABSTRACT An anti-West Nile virus (anti-WNV) monoclonal antibody, SHW-7A11, was developed for competitive enzyme-linked immunosorbent assays (c-ELISAs). SHW-7A11 reacted with nonstructural protein 1 in Western blot analysis. relatively specific the WNV strain NY99 and recognized Kunjin Eg101 strains indirect ELISAs. Two c-ELISAs were sera diluted 10 100 times named c-ELISA10 c-ELISA100, respectively. Both detected antibodies against strains. Little cross-reactivity observed Japanese...
In the present study, group-specific antigen VP7 of bluetongue virus (BTV) serotype 21 isolated from cattle in Tochigi prefecture Japan 1994 was characterized by sequencing and expression. Gene amplified cDNA synthesized on viral dsRNA using reverse-transcriptase-PCR. Nucleotide sequence this isolate showed high similarity with other published BTV sequences. Full-length C-terminal truncated forms were expressed insect cells a baculovirus gene expression system under control polyhedrin...
ABSTRACT West Nile virus (WNV) is endemic throughout Africa, Eurasia, America, and Australia has important implications for avian, horse, human health. In these regions, dead birds are monitored the presence of WNV through immunohistochemistry (IHC) PCR. However, a number tools IHC inadequate owing to their cross-reactivity other Japanese encephalitis serogroup viruses. Here we have established eight monoclonal antibodies (MAbs) WNV. Four them bound envelope protein, three nonstructural...
Serotyping is widely used for typing Salmonella during surveillance, and depends on determining the lipopolysaccharide (LPS) O-antigen flagellar protein (H-antigens) components. As highly variable, structurally unique to each serotype, we investigated binding affinities of LPS from serotypes O4 serogroup with specific anti-antigen serum via immunoblot enzyme-linked immunosorbent assays. Since also express factor 12, O12 antiserum was analysis. different showed antisera. Therefore, based...
A single intramammary infusion of recombinant bovine interleukin-8 (IL-8) at 50 μ g/quarter/head, but not 10 induced clinical mastitis in three four cows during the dry-off period, resulting an elevated rectal temperature, redness and swelling mammary gland, extensive polymorphonuclear leukocyte (PMNL) infiltration, milk clot formation from 1 to 28 days post (PI). In secretions mastitic glands, high levels IL-8 were sustained 8 hours PI, peaking 1–3 PI. The leukocyte-derived elastase...
The cross-reactivity of Japanese encephalitis virus (JEV)-immunized chicken sera and West Nile (WNV)-immunized in serological tests, such as the IgG indirect ELISA (IgG-ELISA), hemagglutination inhibition test (HI) plaque reduction neutralization (PRNT), for JEV WNV were examined. mean JEV/WNV ratio IgG-ELISA JEV-immunized was significantly higher than that WNV-immunized (P<0.01). did not cross-react HI. However, all cross-reacted show titer at 90% reduction, showed reduction. Therefore, it...
Three IgG class anti-bovine CXCL8 (bCXCL8) monoclonal antibody (mAb)-secreting hybridomas, SH8-8D7, SH8-12A5 and SH8-2A1, were developed. SH8-8D7 was IgG2a, SH8-2A1 IgG1. All three mAbs detected recombinant bCXCL8 (rbCXCL8) by immunoprecipitation Western blotting. could neutralise the chemotactic activity of rbCXCL8 towards neutrophils. The quantitative ELISA constituted combination biotin-SH8-2A1. detection range 20-1000 pg/mL. A sandwich used to measure native derived from supernatant...
In this study, an immunohistochemical (IHC) technique for detecting Newcastle disease (ND) virus (avian avulavirus 1) using paraffin sections of formalin-fixed tissues was developed by production novel monoclonal antibodies. Mice were immunized with ND isolate APMV1/chicken/Japan/Fukuoka-1/2004 (genotype VII), and 14 mouse hybridoma clones producing IgG antibodies against the established. Among these antibodies, only three reacted viral antigen on sections, while one (22C483) exhibited...
A 42-year-old woman with early-stage cancer of the esophagogastric junction was admitted to our hospital for treatment. Ambulatory endoscopic examination revealed a round, elevated lesion, 6 × 5 mm in size, reddish surface on top 2-cm polyp ([Fig. 1]) and evidence gastroesophageal reflex disease (GERD).