A5012249800- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Neonatal and Maternal Infections
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Influenza Virus Research Studies
- Infective Endocarditis Diagnosis and Management
- Immune Response and Inflammation
- Otolaryngology and Infectious Diseases
- Bacterial Identification and Susceptibility Testing
- Sepsis Diagnosis and Treatment
- Inflammasome and immune disorders
- Orthopedic Infections and Treatments
- Eicosanoids and Hypertension Pharmacology
- Bacterial Infections and Vaccines
- Orthopaedic implants and arthroplasty
- Toxin Mechanisms and Immunotoxins
- Bone Tissue Engineering Materials
- Plant Pathogenic Bacteria Studies
- Cytokine Signaling Pathways and Interactions
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacterial biofilms and quorum sensing
- Kawasaki Disease and Coronary Complications
- Immunotherapy and Immune Responses
- Ocular Infections and Treatments
Universität Greifswald
2017-2025
Siemens (Germany)
2022
Karolinska University Hospital
2016-2019
Karolinska Institutet
2015-2019
National Center for Infectious Diseases
2015-2016
University of Rostock
2010-2013
Necrotizing fasciitis caused by group A streptococcus (GAS) is a life-threatening, rapidly progressing infection. At present, biofilm not recognized as potential problem in GAS necrotizing soft tissue infections (NSTI), it typically linked to chronic or associated with foreign devices. Here, we present case of previously healthy male presenting NSTI GAS. The infection persisted over 24 days, and the surgeon documented presence "thick layer biofilm" fascia. Subsequent analysis patient...
The entry into epithelial cells and the prevention of primary immune responses are a prerequisite for successful colonization subsequent infection human host by Streptococcus pyogenes (group A streptococci, GAS). Here, we demonstrate that interaction GAS with plasminogen promotes an integrin-mediated internalization bacteria keratinocytes, which is independent from serine protease activity potentially generated plasmin. α(1)β(1)- α(5)β(1)-integrins were identified as major keratinocyte...
Staphylococcus aureus necrotizing pneumonia is recognized as a toxin-mediated disease, but yet the tissue destructive events remain elusive partly due to lack of mechanistic studies in human lung tissue. In this study, 3D model composed epithelial cells and fibroblasts was used delineate role specific staphylococcal exotoxins pathology associated with severe pneumonia. To end, models were exposed mixture produced by S. strains isolated from patients varying severity infection, namely or...
Abstract Neutrophils are critical for the control of bacterial infections, but they may also contribute to disease pathology. Here we explore neutrophil responses, in particular release sepsis-associated factors heparin-binding protein (HBP) and resistin relation specific stimuli sepsis varying aetiology. Analyses HBP plasma septic patients revealed elevated levels as compared non-infected critically ill patients. correlated significantly patients, with strongest association seen group A...
Epithelial cells play a crucial role in detection of the pathogens as well initiation host immune response<i>. Streptococcus pneumoniae</i> (pneumococcus) is typical colonizer human nasopharynx, which can disseminate to lower respiratory tract and subsequently cause severe invasive diseases such pneumonia, sepsis, meningitis. Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) produced by pneumococci product pyruvate oxidase SpxB. However, its virulence...
Abstract Streptococcus dysgalactiae subsp. equisimilis (SDSE) has emerged as an important cause of severe skin and soft tissue infections, but little is known the pathogenic mechanisms underlying pathology. Patient samples a collection invasive non-invasive group G SDSE strains (n = 69) were analyzed with respect to virulence factor expression cytotoxic or inflammatory effects on human cells 3D models. efficiently infected 3D-skin model pathology, responses altered production host structural...
Host genetic variations play an important role in several pathogenic diseases, and we have previously provided strong evidences that these contribute significantly to differences susceptibility clinical outcomes of invasive Group A Streptococcus (GAS) infections, including sepsis necrotizing soft tissue infections (NSTIs). Our initial studies with conventional mouse strains revealed host sex orchestrating the severity, NSTIs. To understand complex architecture NSTIs, utilized unbiased,...
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of skin and soft tissue infections. One the highly successful rapidly disseminating clones MRSA ST22 commonly associated with tropism. Here we show that naturally occurring single amino acid substitution (tyrosine to cysteine) at position 223 AgrC determines starkly different S. virulence phenotypes, e.g. cytotoxic or colonizing, as evident in both vitro vivo Y223C destabilizes AgrC-AgrA interaction leading colonizing...
Abstract In recent years, increased numbers of severe Streptococcus dysgalactiae subsp. equisimilis (SDSE) infections, including necrotizing soft tissue infections (NSTIs), have been reported. One the main virulence factors SDSE is streptokinase (Ska). Ska promotes bacterial spread in through Ska-plasminogen interactions and subsequent activation plasminogen to plasmin. this study, impact on human monocyte-derived dendritic cells (moDCs) was investigated. MoDCs were infected with strain S118...
Introduction: Streptococcus pyogenes (group A streptococcus, GAS) is an exclusively human pathogen. It causes a wide spectrum of diseases, ranging from mild infections such as pharyngitis to severe life-threatening conditions streptococcal toxic shock syndrome (STSS). Thrombocytopenia common feature STSS and associated with outcome. GAS produce plethora virulence factors, including streptolysin S (SLS), which has lytic well immuno-modulatory properties. However, its role in platelet...
ABSTRACT Streptococcus pyogenes (group A streptococcus [GAS]) is a highly virulent Gram-positive bacterium. For successful infection, GAS expresses many virulence factors, which are clustered together with transcriptional regulators in distinct genomic regions. Ralp3 central regulator of the ERES region. In this study, we investigated role M49 pathogenesis. The inactivation resulted reduced attachment to and internalization into human keratinocytes. Δ ralp3 mutant failed survive blood serum,...
Reports have shown that the antimicrobial peptide LL-37 is abundantly expressed but has limited bactericidal effect in <i>Streptococcus pyogenes</i> infections. At sub-inhibitory concentrations, been reported to alter virulence gene expression. Here, we explored interaction of <i>S. strains with LL-37, focusing on bacterial growth, cell surface alterations and pro-inflammatory responses. Bioscreen turbidity measurements strain 5448 cultured presence or absence confirmed...
Several lactic acid bacteria use homolactic fermentation for generation of ATP. Here we studied the role lactate dehydrogenase enzyme on general physiology three Lactococcus lactis, Enterococcus faecalis, and Streptococcus pyogenes. Of note, deletion ldh genes hardly affected growth rate in chemically defined medium under microaerophilic conditions. However, was rich medium. Furthermore, ability utilization various substrates as a carbon source. A switch to mixed observed during...
Abstract Seasonal Influenza A virus (IAV) infections can promote dissemination of upper respiratory tract commensals such as Streptococcus pneumoniae to the lower resulting in severe life-threatening pneumonia. Here, we aimed compare innate immune responses lungs healthy colonized and non-colonized mice after IAV challenge at initial asymptomatic stage infection. Responses during a bacterial pneumonia were profiled for comparison. Cytokine cell imprints analyzed. Irrespective colonization...
Pneumonia is one of the leading causes death worldwide. Here, we compared impact bacterial- and viral-induced pneumonia on respiratory gastrointestinal microbiome. Using a meta-omics approach, identified specific profiles that allow discrimination between bacterial viral causative.
A series of new glycoconjugated zinc(II) phthalocyanines (Pcs) was synthesized and fully characterized. The major focus the structural design for these Pcs upon reduction aggregate formation in solution. Therefore, aglycons Pc‐linked sugars, number position carbohydrate substituents on Pc linker between sugar were varied. Specifically, di‐ octasubstituted with triazole C–C linkages their aggregation behaviour investigated by UV/Vis‐, CD‐ NMR spectroscopy. C‐glycosidically decorated described...
Neutrophils have been proposed as important contributors to the hyperinflammatory responses that are associated with severe invasive Streptococcus pyogenes infections. In particular, streptococcal surface proteins implicated potent neutrophil activators. Here we explore impact of streptococcus-secreted factors on activation and degranulation. Primary human neutrophils were exposed supernatants prepared from cultures S. strains varying serotypes in stationary growth phase. Neutrophil was...
A prominent feature of severe streptococcal infections is the profound inflammatory response that contributes to systemic toxicity. In sepsis dysregulated host involves both immunological and nonimmunological pathways. Here, we report a fatal case an immunocompetent healthy female presenting with toxic shock purpura fulminans caused by group B streptococcus (GBS; serotype III, CC19). The strain (LUMC16) was pigmented hyperhemolytic. Stimulation human primary cells hyperhemolytic LUMC16...