We have previously identified a glycoprotein of the inter-alpha-trypsin inhibitor family proteins as serum factor responsible for stabilization expanding cumulus mass. In this study, mechanism interaction extracellular matrix stabilizing (cESF) with hyaluronic acid (HA) has been explored. It was found that pH optimum binding cESF and HA is 7 sensitive to ionic strength. The dissociation constant about 1.9 x 10(-8) M in 10 mM sodium phosphate buffer (pH 7.2). Circular dichroism studies show...

We tested the hypothesis that apolipoproteins, protein constituents of plasma lipoproteins, are secreted into bile.We examined human gallbladder bile obtained at surgery (N = 54) from subjects with 44) and without ( N 10) gallstones hepatic collected by T-tube drainage 9) after cholecystectomy.Using specific radioimmunoassays for apolipoproteins A-I A-11, major apoproteins high density C-I1 C-111, very low apolipoprotein B, apoprotein we found immunoreactivity these five in every sample...

Purpose: In this study, we aimed to develop a novel liver function and inflammatory markers-based nomogram predict recurrence-free survival (RFS) for AFP-negative (< 20 ng/mL) HCC patients after curative resection. Patients Methods: A total of 166 pathologically confirmed were included at the Ningbo Medical Center Lihuili Hospital. LASSO regression analysis was used data dimensionality reduction element selection. Univariate multivariate Cox analyses performed identify independent risk...

Probucol is a drug that lowers plasma cholesterol in both humans and animals. In low density lipoprotein (LDL) receptor-deficient Watanabe rabbits, probucol reduces the progression of atherosclerosis. This effect may be attributed to antioxidant and/or cholesterol-lowering properties drug. present report we studied antiatherogenic analogue (MDL 29,311) possesses activity but does not lower cholesterol. Modified rabbits (11-12 weeks age) produced by crossing British Brown Japanese were fed...

Hepatic triglyceride lipase (H-TGL) was isolated from human postheparin plasma by column chromatography on heparin-Sepharose and phenyl-Sepharose immunoaffinity with monoclonal antibodies. The purified enzyme had an apparent molecular weight of 65,000 sodium dodecyl sulfate-polyacrylamide gel electrophoresis amino-terminal sequence Leu-Gly-Gln-Ser-Leu-Lys-Pro-Glu. Partial amino acid sequences seven cyanogen bromide peptides were obtained. A hepatoma cDNA library screened synthetic...

The aim of the present study was to investigate whether methylation angiotensin I converting enzyme 2 (ACE2) promoter increases risk essential hypertension (EH). A total 96 patients with EH were recruited and sex‑ age‑matched healthy controls. Methylation 5 CpG dinucleotides in ACE2 quantified using bisulfite pyrosequencing. Logistic regression multiple linear used adjust for confounding factors generalized multifactor dimensionality reduction (GMDR) method applied high‑order interactions....

Inflammation plays a significant role in tumour development, progression, and metastasis. In this study, we focused on comparing the predictive potential of inflammatory markers for overall survival (OS), recurrence-free (RFS), 1- 2-year RFS hepatocellular carcinoma (HCC) patients.

Abstract Modulation of connexin 43 (cx43) in the myometrium timed pregnant rats was studied using enzyme‐linked immunosorbent assay (ELISA), immunocytochemical localization, and immunoblot. These techniques utilized site‐specific antibodies directed against a portion carboxyl tail cx43. We found that cx43 is synthesized several days prior to labor but accumulates within cytoplasm until parturition, when it rapidly transported plasma membrane assembled into gap junction plaques at cell...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTImmunochemistry of human plasma high density lipoproteins. Radioimmunoassay apolipoprotein A-IIS. J. T. Mao, A. M. Gotto, Jr., and R. L. JacksonCite this: Biochemistry 1975, 14, 18, 4127–4131Publication Date (Print):September 1, 1975Publication History Published online1 May 2002Published inissue 1 September 1975https://pubs.acs.org/doi/10.1021/bi00689a033https://doi.org/10.1021/bi00689a033research-articleACS PublicationsRequest reuse...

Lipidomics reavealed the differences of lipid signitures in NASH models induced by MCD and CDAHFD mice. The hepatic PCs, ACs their metabolism CDAHFD-treated mice were down-regulated, mimicking human NASH.

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMechanism of lipid-protein interaction in the plasma lipoproteins: lipid-binding properties synthetic fragments apolipoprotein A-IIS. J. T. Mao, Sparrow, E. B. Gilliam, A. M. Gotto, Jr., and R. L. JacksonCite this: Biochemistry 1977, 16, 19, 4150–4156Publication Date (Print):September 1, 1977Publication History Published online1 May 2002Published inissue 1 September...

The issue of unplanned reoperations poses significant challenges within healthcare systems, with assessing their impact being particularly difficult. current study aimed to assess the influence on hospitalized patients by employing diagnosis-related group (DRG) comprehensively consider intensity and complexity different medical services. A retrospective cohort surgical was conducted at a large tertiary hospital two districts data sourced from DRG database. Hospital length stay (LOS)...

Hepatitis B virus (HBV) remains an important risk factor for hepatocellular carcinoma (HCC), and inflammation plays essential role in tumor development. This study aimed to investigate the impact of inflammatory markers postoperative outcomes patients with HBV-related HCC, providing valuable prognostic indicators after hepatectomy. We retrospectively analyzed 222 HCC surgical resection. The ROC curve was used calculate biomarker cutoff values. Kaplan-Meier method estimate overall survival...

Abstract We describe two techniques for radioimmunoassay of apolipoprotein A-I (apoA-I) in human plasma, each involving use a non-ionic detergent, Tween-20, to expose antigenic sites, and one "IgG SORB" (a suspension killed staphylococci) as solid-phase separator. Tween-20 (3.75 g/L) decreased nonspecific binding unmasked the sites on apoA-I molecule plasma same extent did tedious delipidation procedure, without altering affinity between antibodies determined by Scatchard analysis (Ka...

We aimed to develop a novel preoperative nomogram predict lymph node metastasis (LNM) in perihilar cholangiocarcinoma (pCCA) patients.

Four monoclonal antibodies (IgG2b) to human plasma low-density lipoproteins (LDL) have been characterized. The binding affinities of each antibody 125I-labeled LDL were moderately high, ranging from 10(8) 10(10) L/mol at 4 degrees C, but reduced by least 50-70% 37 C. maximum was unique, 20 95% total LDL, suggesting that particles immunochemically heterogeneous. One antibody, LP-34, had both high and low LDL. Another, LP-47, exhibited affinity for isolated yet reacted poorly with native in...

The purpose of the present study was to investigate whether methylation angiotensin II type 1 receptor&lt;i&gt; (AGTR1)&lt;/i&gt; promoter contributed risk essential hypertension (EH). A total 96 EH cases and gender- age-matched healthy controls were recruited. Methylation 8 CpG dinucleotides (CpG1-8) in &lt;i&gt;AGTR1&lt;/i&gt; examined using bisulphite pyrosequencing technology. Three (CpG6-8) could not be well sequenced, therefore only remaining 5 sites analysed. significantly lower CpG1...

Adverse events (AEs) are a significant concern for healthcare systems. However, it is difficult to evaluate their influence because of the complexity various medical services. This study aimed assess AEs on outcomes hospitalized patients using diagnosis-related group (DRG) database. We conducted case–control at multi-district tertiary hospital with 2200 beds in China, data from DRG An AE refers an unintended physical injury caused or contributed by care that requires additional...