A5012493982- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Circular RNAs in diseases
- COVID-19 Clinical Research Studies
- Atherosclerosis and Cardiovascular Diseases
- Blood Coagulation and Thrombosis Mechanisms
- Blood groups and transfusion
- Protease and Inhibitor Mechanisms
- Retinal Development and Disorders
- Coronary Interventions and Diagnostics
- Biomarkers in Disease Mechanisms
- Blood properties and coagulation
- Acute Myocardial Infarction Research
- Cardiac Arrest and Resuscitation
- Receptor Mechanisms and Signaling
- Angiogenesis and VEGF in Cancer
- Pancreatitis Pathology and Treatment
- Electrospun Nanofibers in Biomedical Applications
- Pancreatic function and diabetes
- Chronic Myeloid Leukemia Treatments
- Diabetes Treatment and Management
- RNA modifications and cancer
King's College London
2020-2024
Medical University of Vienna
2023-2024
British Heart Foundation
2020-2023
Innsbruck Medical University
2016-2017
Universität Innsbruck
2016
Good Samaritan Hospital
1991
Prognostic characteristics inform risk stratification in intensive care unit (ICU) patients with coronavirus disease 2019 (COVID-19). We obtained blood samples (n = 474) from hospitalized COVID-19 123), non-COVID-19 ICU sepsis 25) and healthy controls 30). Severe acute respiratory syndrome 2 (SARS-CoV-2) RNA was detected plasma or serum (RNAemia) of when neutralizing antibody response low. RNAemia is associated higher 28-day mortality (hazard ratio [HR], 1.84 [95% CI, 1.22-2.77] adjusted for...
Rhodopsin kinase (RK) catalyzes the phosphorylation of rhodopsin (Rho) as one steps in quenching photoactivated Rho. In this work, we investigated autophosphorylation RK and how it affects interaction between undergoes intramolecular phosphorylation, resulting incorporation three or four phosphates per molecule. Phosphorylated subsequently is a substrate for protein phosphatases 2A 2B. We isolated forms based on their differential interactions with heparin-Sepharose. Fully phosphorylated...
Abstract Aims Coronavirus disease 2019 (COVID-19) can lead to multiorgan damage. MicroRNAs (miRNAs) in blood reflect cell activation and tissue injury. We aimed determine the association of circulating miRNAs with COVID-19 severity 28 day intensive care unit (ICU) mortality. Methods results performed RNA-Seq plasma healthy controls (n = 11), non-severe 18), severe 18) patients selected 14 according cell- origin for measurement by reverse transcription quantitative polymerase chain reaction...
Objective: Platelets are central to acute myocardial infarction (MI). How the platelet proteome is altered during MI unknown. We sought describe changes in and identify corresponding functional consequences. Approach Results: from patients experiencing ST-segment–elevation (STEMI) before 3 days after treatment (n=30) matched with severe stable coronary artery disease bypass grafting (n=25) underwent quantitative proteomic analysis. Elevations proteins S100A8 S100A9 were detected at time of...
Platelets contain non-coding RNAs (ncRNAs), and their measurement may complement platelet aggregometry. In the community-based Bruneck Study (n = 338), we generated platelet-rich plasma (PRP), platelet-poor (PPP) platelets. PRP was subjected to aggregometry using various agonists processed releasates thereafter. Releasates, PPP platelets underwent real-time polymerase chain reactions measure ncRNAs. Platelet ncRNA release appeared agonist-specific, dose-dependent, inhibited by aspirin....
Introduction Bariatric surgery offers the most effective treatment for obesity, ameliorating or even reverting associated metabolic disorders, such as type 2 diabetes. We sought to determine effects of bariatric on circulating microRNAs (miRNAs) that have been implicated in cross talk between liver and adipose tissue. Research design methods measured 30 miRNAs 155 morbidly obese patients 47 controls defined associations parameters. Patients were followed up 12 months after surgery. Key...
The effect of the PCSK9 (proprotein convertase subtilisin/kexin type 9) inhibitor alirocumab on platelet aggregation among patients with acute myocardial infarction (AMI) remains unknown. We aimed to explore added high-intensity statin therapy P2Y12 reaction unit (PRU) AMI receiving dual antiplatelet (DAPT) a potent (ticagrelor or prasugrel). In addition, we assessed circulating platelet-derived noncoding RNAs (microRNAs and YRNAs).
There is evidence for the effects of platelet inhibition on innate immune activation. Circulating microRNAs (miRNAs) have been implicated as markers and leukocyte In present study, we assessed P2Y12 inhibitors miRNAs during endotoxemia. Healthy volunteers were randomly assigned to receive oral ticagrelor (n = 10), clopidogrel 8) or no drug one week, followed by an intravenous bolus 2 ng/kg endotoxin. Serum was collected at baseline, after week antiplatelet treatment 6 24 h endotoxin...
Data on perioperative extracorporeal membrane oxygenation (ECMO) in liver transplantation (LT) are scarce. ECMO has been used preoperatively, intraoperatively, and postoperatively for a variety of indications at our center. This retrospective, single-center study use peri-LT aimed to describe predictors successful outcome this highly select cohort patients. Demographics, support method, indication LT were compared between survivors nonsurvivors. Twenty-nine patients received venovenous (V-V;...
The proportion of young platelets, also known as newly formed or reticulated, within the overall platelet population has been clinically correlated with adverse cardiovascular outcomes. However, our understanding this is incomplete because limitations in technical approaches available to study platelets different ages. In study, we have developed and validated an vivo temporal labeling approach using injectable fluorescent antiplatelet antibodies subdivide by age assess differences...
Abstract Arterial thrombosis contributes to some of the most frequent causes mortality globally, such as myocardial infarction and stroke. Platelets are essential mediators physiological haemostasis pathological thrombosis. Platelet activation is controlled by a multitude signalling pathways. Upon activation, platelets shed platelet‐derived extracellular vesicles (pEVs). In this Special Issue: Extracellular Vesicles, Moon et al. investigate impact various platelet agonists (thrombin, ADP,...
Abstract Prognostic characteristics inform risk stratification in intensive care unit (ICU) patients with coronavirus disease 2019 (COVID-19). We obtained blood samples (n = 474) from hospitalized COVID-19 123), non-COVID-19 ICU sepsis 25) and healthy controls 30). Severe acute respiratory syndrome 2 (SARS-CoV-2) RNA was detected plasma or serum (RNAemia) of when neutralizing antibody response low. RNAemia associated higher 28-day mortality (hazard ratio [HR], 1.84 [95% CI, 1.22–2.77]...
Abstract Rationale: Early prognostication after out-of-hospital cardiac arrest (OHCA) is an unmet clinical need. There limited data on serial release kinetics of cardiac, brain and systemic biomarkers such as Neuron Specific Enolase (NSE) S-100B. miRNAs are sensitive biomarker candidates in acute myocardial infarction (MI). Objective The objective was to characterise circulating biomolecules the early OHCA, compared with a STEMI control group assessed outcome. Methods Results Serial blood...
Abstract Funding Acknowledgements Type of funding sources: Foundation. Main source(s): British Heart Foundation (BHF), VASCage (Centre for Promoting Vascular Health in the Ageing Community) Austrian Research Promotion Agency FFG (COMET program - Competence Centers Excellent Technologies) Introduction Antiplatelet therapy (APT) leads to reduced morbidity and mortality patients with cardiovascular disease but some still have thrombotic events. Tailoring APT platelet function is currently...
Abstract Funding Acknowledgements Type of funding sources: None. Background/Introduction Circulating small non-coding RNAs (ncRNAs), such as microRNAs (miRNAs) and YRNAs, have been implicated in intercellular communication biomarkers for cardiometabolic diseases. The putative carriers ncRNAs are lipoproteins, extracellular vesicles (EVs) RNA-binding proteins. However, the lack reliable separation methods EVs lipoproteins from human blood hampers assignment ncRNA signatures to different...
Abstract Background Antiplatelet therapy (APT) has improved cardiovascular outcomes, but some patients develop thrombosis despite APT. Adjusting APT to platelet reactivity with conventional tests proven unsuccessful. Many circulating noncoding RNAs (ncRNAs) are derived from platelets and could serve as novel function markers. Material methods Platelet was assessed using light transmission aggregometry (LTA) in the Bruneck 2015 study (N = 338), agonists arachidonic acid, adenosine...