A5004144902- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- COVID-19 Clinical Research Studies
- Viral Infections and Outbreaks Research
- Zoonotic diseases and public health
- Immunotherapy and Immune Responses
- Plant Virus Research Studies
- Influenza Virus Research Studies
- Viral Infections and Vectors
- Respiratory Support and Mechanisms
- RNA regulation and disease
- Immune responses and vaccinations
- Monoclonal and Polyclonal Antibodies Research
- Vaccine Coverage and Hesitancy
- Nuclear Structure and Function
- Dental Research and COVID-19
- vaccines and immunoinformatics approaches
- Cystic Fibrosis Research Advances
Centro Nacional de Biotecnología
2016-2023
Universidad Autónoma de Madrid
2016-2021
Consejo Superior de Investigaciones Científicas
2018-2021
Viroporins are viral proteins with ion channel (IC) activity that play an important role in several processes, including virus replication and pathogenesis. While many coronaviruses (CoVs) encode two viroporins, severe acute respiratory syndrome CoV (SARS-CoV) encodes three: 3a, E, 8a. Additionally, 3a E have a PDZ-binding motif (PBM), which can potentially bind over 400 cellular contain PDZ domain, making them for the control of cell function. In present work, comparative study functional...
The coronavirus spike glycoprotein, located on the virion surface, is key mediator of cell entry and focus for development protective antibodies vaccines. Structural studies show exposed sites trimer that might be targeted by with cross-species specificity. Here we isolated two human monoclonal from immunized humanized mice display a remarkable cross-reactivity against distinct proteins betacoronaviruses including SARS-CoV, SARS-CoV-2, MERS-CoV endemic HCoV-OC43. Both cross-reactive target...
The Middle-East respiratory syndrome coronavirus (MERS-CoV) is a zoonotic virus that causes severe and often fatal disease in humans. Efforts to develop antibody-based therapies have focused on neutralizing antibodies target the receptor binding domain of viral spike protein thereby blocking binding. Here, we developed set human monoclonal functionally distinct domains MERS-CoV protein. These belong six epitope groups interfere with three critical entry functions protein: sialic acid...
Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel human that emerged in 2012, causing severe pneumonia and acute distress (ARDS), with case fatality rate of ~36%. When expressed isolation, CoV accessory proteins have been shown to interfere innate antiviral signaling pathways. However, there limited information on the specific contribution MERS-CoV protein 4b repression response context infection. We found was required prevent robust NF-κB dependent during In wild-type virus...
Middle East respiratory syndrome coronavirus (MERS-CoV) continues to cause outbreaks in humans as a result of spillover events from dromedaries. In contrast humans, MERS-CoV-exposed dromedaries develop only very mild infections and exceptionally potent virus-neutralizing antibody responses. These strong responses may be caused by affinity maturation repeated exposure the virus or fact that dromedaries-apart conventional antibodies-have relatively unique, heavy chain-only antibodies (HCAbs)....
Coronaviruses (CoVs) of genera α, β, γ, and δ encode proteins that have a PDZ-binding motif (PBM) consisting the last four residues envelope (E) protein (PBM core). PBMs may bind over 400 cellular containing PDZ domains (an acronym formed by combination first letter names three where this domain was identified), making them relevant for control cell function. Three highly pathogenic human CoVs been identified to date: severe acute respiratory syndrome coronavirus (SARS-CoV) Middle East...
The emergence of the new highly pathogenic human coronavirus SARS-CoV-2 that has already infected more than 80 million persons, killing nearly two them, clearly indicates need to design efficient and safe vaccines protecting from these coronaviruses. Modern can be derived virus-host interaction research directed identification signaling pathways essential for virus replication virus-induced pathogenesis, in order learn how attenuate viruses vaccines.
Abstract The coronavirus spike glycoprotein, located on the virion surface, is key mediator of cell entry. As such, it an attractive target for development protective antibodies and vaccines. Here we describe two human monoclonal antibodies, 1.6C7 28D9, that display a remarkable cross-reactivity against distinct species from three Betacoronavirus subgenera, capable binding proteins SARS-CoV SARS-CoV-2, MERS-CoV endemic HCoV-OC43. Both derived immunized transgenic mice carrying immunoglobulin...
Self-amplifying RNA replicons are promising platforms for vaccine generation. Their defects in one or more essential functions viral replication, particle assembly, dissemination make them highly safe as vaccines. We previously showed that the deletion of envelope (E) gene from Middle East respiratory syndrome coronavirus (MERS-CoV) produces a replication-competent propagation-defective replicon (MERS-CoV-ΔE). Evaluation this mice expressing human dipeptidyl peptidase 4, virus receptor,...
Middle East respiratory syndrome coronavirus (MERS-CoV) causes a highly lethal pneumonia that emerged in 2012. There is limited information on MERS-CoV pathogenesis, as data from patients are scarce and the generation of animal models reproducing MERS clinical manifestations has been challenging. Human dipeptidyl peptidase 4 knock-in (hDPP4-KI) mice mouse-adapted strain (MERSMA-6-1-2) were recently described. hDPP4-KI infected with MERSMA-6-1-2 show pathological signs disease, high viral...