A5046971987- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- Extracellular vesicles in disease
- Virology and Viral Diseases
- Viral Infections and Vectors
- Pancreatic function and diabetes
- Bacteriophages and microbial interactions
- Cytomegalovirus and herpesvirus research
- Neurogenesis and neuroplasticity mechanisms
- Genetics and Neurodevelopmental Disorders
- Viral Infections and Immunology Research
- Muscle Physiology and Disorders
- RNA regulation and disease
- Metabolism and Genetic Disorders
- Antimicrobial Peptides and Activities
- T-cell and B-cell Immunology
- Reproductive System and Pregnancy
- Viral Infections and Outbreaks Research
- Growth Hormone and Insulin-like Growth Factors
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Zebrafish Biomedical Research Applications
- Hypothalamic control of reproductive hormones
Centro de Biología Molecular Severo Ochoa
2008-2024
Universidad Autónoma de Madrid
2001-2021
Consejo Superior de Investigaciones Científicas
2001-2016
Instituto Cajal
1991-1992
Centro de Investigaciones Biológicas Margarita Salas
1987-1988
A deletion mutant of severe acute respiratory syndrome coronavirus (SARS-CoV) has been engineered by deleting the structural E gene in an infectious cDNA clone that was constructed as a bacterial artificial chromosome (BAC). The recombinant virus lacking (rSARS-CoV-DeltaE) rescued Vero E6 cells. recovered grew E6, Huh-7, and CaCo-2 cells to titers 20-, 200-, 200-fold lower than wild-type virus, respectively, indicating although protein effect on growth, it is not essential for replication....
Herpes simplex virus 1 (HSV-1) is a neurotropic pathogen that can infect many types of cells and establishes latent infections in the neurons sensory ganglia. In some cases, spreads into central nervous system, causing encephalitis or meningitis. Cells infected with several different viruses may secrete microvesicles (MVs) containing viral proteins RNAs. instances, extracellular harboring infectious have been found. Here we describe features shedding released by human oligodendroglial HOG...
Abstract We have previously shown that in hypothalamic mixed neuronal‐glial cultures both astrocytic shape and distribution of glial fibrillary acidic protein (GFAP) are modified by estradiol. In the present study, we investigated whether or not presence neurons is necessary for these hormonal effects. proportion process‐bearing GFAP‐immunoreactive cells was significantly increased after treatment 30 min with 10 −12 M 17β This effect at least 1 day reverted incubating estradiol‐free medium....
The human cytomegalovirus (HCMV) has been proposed to complete its final envelopment on cytoplasmic membranes prior release the extracellular medium. nature of these and mechanisms involved in virus are poorly understood. Here we show by immunogold-labelling electron microscopy that CD63, a marker multivesicular bodies (MVBs), is incorporated into viral envelope, supporting notion HCMV uses endocytic for envelopment. We therefore investigated possible role cellular endosomal sorting complex...
Abstract Intramuscular injection of Wnt7a has been shown to accelerate and augment skeletal muscle regeneration ameliorate dystrophic progression in mdx muscle, a model for Duchenne muscular dystrophy (DMD). However, loss-of-function studies investigate the requirement not evaluated. Here, we assessed function wild type (WT) mice where was specifically deleted using conditional floxed allele Myf5-Cre driver. We found that both WT with deletion exhibited marked deficiencies at 21 d following...
Alzheimer's disease is a form of senile mental disorder characterized by the presence extracellular plaques, containing amyloid-beta (Abeta) as main component. According to amyloid hypothesis, an increase Abeta production in origin aberrant plaques causing neuronal loss and dementia. However, wealth evidence has been accumulated pointing toxicity soluble intracellular Abeta, having different morphologies aggregation, neurodegenerative process. The exact nature initial molecular events which...
Plus-stranded RNA viruses replicate in the cytosol of infected cells, membrane-bound replication complexes containing replicase proteins, viral and host proteins. The formation transcription (RTCs) through rearrangement cellular membranes is currently being actively studied for belonging to different families. In this work, we identified double-membrane vesicles (DMVs) cytoplasm cells with equine torovirus Berne virus (BEV), prototype member Torovirus genus (Coronaviridae family, Nidovirales...
The production of microcin 17 is controlled by plasmid pRYC17. Chromosomal mutants unable to produce a normal amount were isolated in Escherichia coli. One the mutations maps ompR locus, indicating that an active OmpR product required for synthesis 17. same conclusion was obtained colicin E2. Therefore, two new functions regulatory gene have been revealed.
Self-amplifying RNA replicons are promising platforms for vaccine generation. Their defects in one or more essential functions viral replication, particle assembly, dissemination make them highly safe as vaccines. We previously showed that the deletion of envelope (E) gene from Middle East respiratory syndrome coronavirus (MERS-CoV) produces a replication-competent propagation-defective replicon (MERS-CoV-ΔE). Evaluation this mice expressing human dipeptidyl peptidase 4, virus receptor,...
The Ebola virus VP24 protein plays a critical role in escape of the from host innate immune response. Therefore, deciphering molecular mechanisms modulating activity may be useful to identify potential targets amenable therapeutics. Here, we cellular proteins USP7, SUMO, and ubiquitin as novel interactors regulators VP24. These interactions represent design new antivirals with ability modulate replication.
Members of the Bacillus genus, particularly “Bacillus subtilis group”, are known to produce amphipathic lipopeptides with biosurfactant activity. This includes surfactins, fengycins and iturins that have been associated antibacterial, antifungal, anti-viral properties. We screened a large collection Bacillus, isolated from human, animal, estuarine water soil samples found most potent lipopeptide producers members species velezensis. B. velezensis exhibited anti-bacterial activity which was...
Intramuscular injection of Wnt7a has been shown to accelerate and augment skeletal muscle regeneration ameliorate dystrophic progression in mdx muscle, a model for Duchenne muscular dystrophy (DMD). Here, we assessed function wild type (WT) mice where was deleted using conditional floxed allele Myf5-Cre driver. We found that both WT lacking exhibited marked deficiencies at 21 d following cardiotoxin (CTX) induced injury. Unlike WT, deletion resulted decreased force generation prior CTX...
The velocity of the nerve impulse conduction vertebrates relies on myelin sheath, an electrically insulating layer that surrounds axons in both central and peripheral nervous systems, enabling saltatory action potential. Oligodendrocytes are myelin-producing glial cells system. A deeper understanding molecular basis myelination and, specifically, transport proteins, will contribute to search aetiology many dysmyelinating demyelinating diseases, including multiple sclerosis. Recent...
In the mouse embryo, hematopoietic progenitor cells migrate to fetal liver (FL) between gestational days (E) 9.5 and 10.5, where they rapidly expand form main reservoir of cells. The embryonic megakaryocyte progenitors (MKPs) in E11.5 FL were identified as CD49fHCD41H (and c-KitDKDR+CD42+CD9++CD31+) cells, expressing several hepato-specific proteins. Unlike adult bone marrow megakaryocytes (MKs), MKPs CD45− represent an abundant population FL. purified by cytometry differentiated vitro...