A5008413355- Ubiquitin and proteasome pathways
- Polyamine Metabolism and Applications
- interferon and immune responses
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Viral Infections and Outbreaks Research
- RNA regulation and disease
- Poxvirus research and outbreaks
- Viral Infections and Vectors
- Mast cells and histamine
- Mitochondrial Function and Pathology
- Telomeres, Telomerase, and Senescence
- Herpesvirus Infections and Treatments
- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- Cytomegalovirus and herpesvirus research
- Biochemical and Molecular Research
- Nuclear Structure and Function
- Hepatitis B Virus Studies
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- Parvovirus B19 Infection Studies
- PI3K/AKT/mTOR signaling in cancer
- RNA modifications and cancer
Icahn School of Medicine at Mount Sinai
2024
Instituto de Investigación Sanitaria de Santiago
2017-2024
Universidade de Santiago de Compostela
2017-2022
Center for Research in Molecular Medicine and Chronic Diseases
2019-2020
Universitat de Barcelona
1984
Abstract The human transcriptome contains thousands of small open reading frames (sORFs) that encode microproteins whose functions remain largely unexplored. Here, we show TINCR lncRNA encodes pTINCR, an evolutionary conserved ubiquitin-like protein (UBL) expressed in many epithelia and upregulated upon differentiation under cellular stress. By gain- loss-of-function studies, demonstrate pTINCR is a key inducer epithelial vitro vivo. Interestingly, low expression associates with worse...
Abstract Background The eukaryotic translation initiation protein eIF5A is a highly conserved and essential factor that plays critical role in different physiological pathological processes including stress response cancer. Different proteomic studies suggest may be small ubiquitin-like modifier (SUMO) substrate, but whether indeed SUMOylated how relevant this modification for activities are still unknown. Methods SUMOylation was evaluated using vitro assays, Histidine-tagged proteins...
The ribosomal protein L11 (RPL11) integrates different types of stress into a p53-mediated response. Here, we analyzed the impact ubiquitin-like SUMO on RPL11-mouse double-minute 2 homolog-p53 signaling. We show that small ubiquitin-related modifier (SUMO)1 and SUMO2 covalently modify RPL11. find negatively modulates conjugation neural precursor cell-expressed developmentally downregulated 8 (NEDD8) to RPL11 promotes translocation RP outside nucleoli. Moreover, SUMO-conjugating enzyme, Ubc9,...
ABSTRACT The 2022–2024 outbreak of MPOX is an important worldwide public health issue that has triggered significant concerns in the scientific community. caused by monkeypox virus (MPXV) belonging to Poxviridae family. study MPXV presents a multifaceted challenge due diverse viral formThis was supported ISIDORe consortium and Agencia Estatal de Investigación.s produced this pathogen. Notably intracellular mature viruses (MVs) primarily contribute localized lesions host‐to‐host transmission,...
The Ebola virus (EBOV) VP24 protein is a nucleocapsid-associated with multiple functions. Proteomic studies have identified the cellular nuclear membrane component emerin as potential interactor.
The Ebola virus VP24 protein plays a critical role in escape of the from host innate immune response. Therefore, deciphering molecular mechanisms modulating activity may be useful to identify potential targets amenable therapeutics. Here, we cellular proteins USP7, SUMO, and ubiquitin as novel interactors regulators VP24. These interactions represent design new antivirals with ability modulate replication.
Active hypusine-modified initiation elongation factor 5A is critical for cell proliferation and differentiation, embryonic development, innate immune response of macrophages to bacterial infection. Here, we demonstrate that both virus infection double-stranded RNA viral mimic stimulation induce the hypusination eIF5A. Furthermore, show activation eIF5A essential replication several viruses including influenza A virus, vesicular stomatitis chikungunya mayaro una zika punta toro virus....
Activated dsRNA-dependent serine/threonine kinase PKR phosphorylates the alpha subunit of eukaryotic initiation factor 2 (eIF2α), resulting in a shut-off general translation, induction apoptosis, and inhibition virus replication. can be activated by binding to dsRNA or cellular proteins such as PACT/RAX, its conjugation ISG15 SUMO. Here, we demonstrate that also interacts with SUMO non-covalent manner. We identify phosphorylable tyrosine residue 162 (Y162) modulator PKR-SUMO interaction well...
Polyamines, spermine and spermidine in the micromolar range (1-100 mumol/l) increase ADP epinephrine-induced platelet aggregation vitro. The possible rôle of polyamines as regulating factors physiological pathological states is discussed.