A5039770527- Glioma Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Cancer Research and Treatments
- Ferroptosis and cancer prognosis
- CAR-T cell therapy research
- Neuroinflammation and Neurodegeneration Mechanisms
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Brain Metastases and Treatment
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- Nanoplatforms for cancer theranostics
- 3D Printing in Biomedical Research
- Peroxisome Proliferator-Activated Receptors
- Vitamin D Research Studies
- Ovarian cancer diagnosis and treatment
- Nerve injury and regeneration
- Software-Defined Networks and 5G
- Chromatin Remodeling and Cancer
- Cellular Mechanics and Interactions
- Molecular Biology Techniques and Applications
- Neuroblastoma Research and Treatments
Huashan Hospital
2019-2024
Shanghai Clinical Research Center
2023-2024
Fudan University
2019-2024
Shanghai Center for Brain Science and Brain-Inspired Technology
2023-2024
Xidian University
2019
Shanghai Public Health Clinical Center
2016-2017
State Key Laboratory of Medical Neurobiology
2015-2017
Shanghai Medical College of Fudan University
2015-2017
Abstract Glioma, with its heterogeneous microenvironments and genetic subtypes, presents substantial challenges for treatment prediction development. We integrated 3D bioprinting multi-algorithm machine learning as a novel approach to enhance the assessment understanding of glioma responses microenvironment characteristics. The bioprinted patient-derived tissues successfully recapitulated molecular properties drug native tumors. then developed GlioML, workflow incorporating nine distinct...
Abstract Background The tumor immune microenvironment can influence the prognosis and treatment response to immunotherapy. We aimed develop a non-invasive radiomic signature in high-grade glioma (HGG) predict absolute density of tumor-associated macrophages (TAMs), preponderant cells HGG. also evaluate association between signature, phenotype as well Methods In this retrospective setting, total 379 patients with HGG from three independent cohorts were included construct model named Radiomics...
Glioblastoma survival remains unchanged despite continuing therapeutic innovation. Herein, we aim to (i) develop chimeric antigen receptor (CAR) T cells with a specificity unique antigen, carbonic anhydrase IX (CAIX), which is expressed in the hypoxic microenvironment characteristic of glioblastoma, and (ii) demonstrate its efficacy limited off-target effects.First demonstrated expression CAIX patient-derived glioblastoma samples available databases. CAR were generated against was assessed 4...
Cancer cells commonly have metabolic abnormalities. Aside from altered glucose and amino acid metabolism, cancers often share the attribute of fatty alterations. However, metabolism related-gene set has not been systematically investigated in gliomas. Here, we provide a bioinformatic profiling catabolic metabolism-related gene risk signature for malignancy, prognosis immune phenotype glioma. In this study, cohort 325 patients with whole genome RNA-seq expression data Chinese Glioma Genome...
Background Characterizing expression profiles of different immune checkpoint molecules are promising for personalized inhibitory immunotherapy. Gliomas have been shown as potential targets inhibitors recently. Our study was performed to determine coexpression levels two major B7 regulatory programmed death ligand 1 (PD-L1) and B7-H4, both which demonstrated inhibit antitumor host immunity in gliomas. Methods We assessed tumor tissues from stage II–IV primary gliomas (n=505) by...
The present study focuses on the function of cpg15, a neurotrophic factor, in ischemic neuronal recovery using transient global cerebral (TGI) mouse model and oxygen-glucose deprivation (OGD)-treated primary cultured cells. results showed that expression cpg15 proteins astrocytes, predominantly soluble form, was significantly increased hippocampus after TGI astrocytes OGD. Addition medium from cpg15-overexpressed astrocytic culture into OGD-treated hippocampal cultures reduces injury,...
Ascorbate is a pro-oxidant that generates hydrogen peroxide-dependent cytotoxity in cancer cells without adversely affecting normal cells. To determine the mechanistic basis for this phenotype, we selected Burkitt lymphoma resistant to ascorbate (JLPR cells) and their ascorbate-sensitive parental (JLPS cells). Compared with JLPS cells, increased glucose uptake JLPR (with upregulated transporters, antioxidant enzyme activity, altered cell cycling) conferred ascorbate-induced cytotoxicity...
Glioma is the most malignant primary brain tumor with a poor survival time. The tumour microenvironment, especially glioma-associated microglia/macrophages (GAMs), plays an important role in pathogenesis of glioma. Currently, microglia (CD11b+/CD45Low) and macrophages (CD11b+/CD45High) are distinguished as distinct cell types due to their different origins. Moreover, signal-transducing adaptor protein 1 (STAP1) tumourigenesis immune responses. However, date, no studies have been reported on...
B7-H4 is a promising immune checkpoint molecule in tumor immunotherapy. Our previous study showed that high expression was strongly correlated with deficiency infiltrated lymphocytes (TILs) glioma patients. On this basis, we investigated the impact of on CD8+TILs gliomas and associated molecular mechanism here. B7-H4-positive samples (n=129) from our cohort were used to assess CD8+TIL quantification by immunohistochemistry. five patients cultured protein evaluate anti-tumor dysfunction flow...
ABSTRACT This study focuses on the function of NSSR1, a splicing factor, in neuronal injury ischemic mouse brain using transient global cerebral model and cultured cells treated with oxygen‐glucose deprivation (OGD). The results showed that ischemia triggers expression NSSR1 hippocampal astrocytes, predominantly dephosphorylated proteins, Exon3 inclusive NCAM‐L1 variant Exon4 CREB variant. While hippocampus astrocyte‐specific conditional knockdown (cKD) mice, where no longer were triggered...
2042 Background: Adoptive cell therapy using tumor-infiltrating lymphocytes (TILs) is effective against solid tumors with convincing evidence. But T-cells infused were exhausted when they encountered PD-1-mediated immunosuppression in the microenvironment. Here, we generated a modified autologous TILs secreting antibody targeting PD-1 (PD1-TIL), and conducted first-in-human, two arms, phase 1 study (NCT03347097) recurrent glioblastoma who had poor prognosis estimated 6-9 months of median...
Co-inhibitory immune checkpoints play a crucial role in tumor progression, and PD-1/PD-L1 inhibitor has been breakthrough for treating multiple refractory tumors last decade. Nevertheless, results of several phase III clinical trials are unsatisfactory high grade gliomas recently. This article reviews the promising biomarkers which can predict efficacy blockade immunotherapy current status emerging strategies involving inhibitors, especially combination treatment neoadjuvant PD-1 therapy...
Emerging molecular and genetic biomarkers have been introduced to classify gliomas in the past decades. Here, we a risk signature based on cellular response IL-4 gene set through Least Absolute Shrinkage Selection Operator (LASSO) regression analysis.In this study, provide bioinformatic profiling of our for malignancy, prognosis immune phenotype glioma. A cohort 325 patients with whole genome RNA-seq expression data from Chinese Glioma Genome Atlas (CGGA) dataset was used as training set,...
2059 Background: Our previous study showed A2B5 + glioma stem-like cell lysate-loaded DC vaccine (GSC-DCV) extended survival in recurrent GBM. Additionally, our earlier research indicates that GSCs, enriched with tumor-specific antigens like URGCP, have the potential to activate CD8 T cells via dendritic antigen presentation. Recent on GBM suggest aPD-1 mAb neoadjuvant therapy further extends survival. This is intended assess safety and efficacy of this combined therapeutic approach....
Routing and spectrum allocation (RSA) is an important problem in elastic optical networks (EONs). With the increase of network scale task requests, imbalance topology becomes more prominent, causing congestion some links even paralysation entire network. To tackle this issue, paper, we first set up integer linear programming model for RSA problems EONs, which minimises number used slots, optimises balance burden KeyLinks (i.e., are easy blocked when large requests comes). Then design a new...
Abstract Background Glioma is the most malignant primary brain tumor with a poor survival time. The tumour microenvironment, especially glioma-associated microglia/macrophages (GAMs), plays major role in pathogenesis of glioma. Currently, microglia (CD11b + /CD45 Low ) and macrophages High are distinguished as distinct cell types due to their different origins. Moreover, Signal-transducing adaptor protein 1 (STAP1) tumourigenesis immune responses. However, date, no studies on STAP1 GAMs have...
Abstract Our previous phase II randomized trials have determined that A2B5+ glioma stem-like cell lysate-loaded DC vaccine (GSC-DCV) demonstrates favorable safety and significantly extends the survival of glioblastoma (GBM) (NCT01567202). This study aims to investigate clinical feasibility efficacy GSC-DCV combined with aPD-1 mAbs neoadjuvant therapy in recurrent GBM (NCT04888611). (IDH1/2 -) patients who had received radiotherapy TMZ chemotherapy were enrolled after pathologically confirmed...
INTRODUCTION: The expression profiles of different immune checkpoint molecules are promising for triaging personalized targeted immunotherapy. Our study was performed to determine co-expression levels 2 major B7 molecules, PD-L1 and B7-H4, in gliomas where both have demonstrated inhibit antitumor host immunity. METHODS: We assessed tumor issues from primary stage II IV (n = 505) by immunohistochemistry (IHC) protein B7-H4. Gene analysis assessing clusters based on extent PD-L1/B7-H4...
Abstract Adoptive cell therapies utilizing autologous tumor-infiltrating lymphocytes (TIL) have been demonstrated to be safe and promising in anti-tumor activities; However, their efficiency was still low treating glioblastoma multiforme (GBM) because of immunosuppression microenvironment. In this study, we proposed a new strategy enhance the immune response by using modified TIL expressing anti-PD-1 antibody (anti-PD1-TIL), which could reverse tumor microenviroment. From April 2017 2018，a...