A5086606715- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Insect-Plant Interactions and Control
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Animal Virus Infections Studies
- CRISPR and Genetic Engineering
- Plant Disease Management Techniques
- Plant Parasitism and Resistance
- Plant pathogens and resistance mechanisms
- Bacteriophages and microbial interactions
- Plant Molecular Biology Research
- Agronomic Practices and Intercropping Systems
- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Plant Pathogenic Bacteria Studies
- Plant Reproductive Biology
- Weed Control and Herbicide Applications
- Nematode management and characterization studies
- Herpesvirus Infections and Treatments
- Molecular Biology Techniques and Applications
- Plant Pathogens and Fungal Diseases
- Plant Physiology and Cultivation Studies
Czech Agrifood Research Center
2016-2025
Czech Academy of Sciences, Institute of Experimental Botany
2022-2024
Laboratoire Génome et Développement des Plantes
2007-2008
Institut National de la Recherche Agronomique
2007
Reference genes are commonly used as the endogenous normalisation measure for relative quantification of target genes. The appropriate application quantitative real-time PCR (RT-qPCR), however, requires use reference whose level expression is not affected by test, general physiological conditions or inter-individual variability. For this purpose, seven were investigated in tissues most important cereals (wheat, barley and oats). Titre Barley yellow dwarf virus (BYDV) was determined oats...
The calcium (Ca2+) signaling is a crucial event during plant-herbivore interaction, which involves transient change in cytosolic Ca2+ concentration, sensed by Ca2+-sensors, and the received message transduced to downstream target proteins leading appropriate defense response. Calmodulin-like (CMLs) are calcium-sensing plant-specific proteins. Although CMLs have been identified few plants, they remained uncharacterized leguminous crop plants. Therefore, wide-range analysis of soybean was...
This study investigated the distribution and characteristics of Barley yellow dwarf virus (BYDV) species BYDV‐PAS, which was recently separated from BYDV‐PAV, most commonly studied BYDV species. Throughout 3 years experimental monitoring incidence, PAS frequently occurring infecting cereals grasses in Czech Republic. Furthermore, Rhopalosiphum maidis Metopolophium dirhodum were recorded as BYDV‐PAS vectors, even though M. does not usually transmit BYDV‐PAV. In field experiments with barley...
The plant genome can produce long non-coding RNAs (lncRNAs), some of which have been identified as important regulators gene expression. To better understand the response mechanism rice plants to Rice black-streaked dwarf virus (RBSDV) infection, we performed a comparative transcriptome analysis between RBSDV-infected and non-infected plants. A total 1342 mRNAs 22 lncRNAs were be differentially expressed after RBSDV infection. Most transcripts involved in plant–pathogen interaction pathway...
A survey of Barley yellow dwarf virus (BYDV) incidence in cereal crops the Czech Republic over 4 years showed, on average, 13.3% BYDV-positive, randomly tested wheat and barley samples. The cultivated cultivars had different levels susceptibility to BYDV infection. Field trials were performed with breeding lines cultivars, resistance traits evaluated after artificial inculcation by viruliferous aphid vector Rhopalosiphum padi L. BYDV-PAV. Our results showed high variability visual symptom...
Wheat streak mosaic virus (WSMV) has become a re-emerging pathogen in cereal crops the Czech Republic. WSMV was first reported former Czechoslovakia early 1980s, and then no record of documented until 2009. The incidence recorded recent years several winter wheat fields many grass species. Here, we surveyed crops. results demonstrated existence volunteer during each year monitoring period, which spanned from 2013–2016. Although range infected samples low (6.4% total tested samples), high...
Sharka caused by Plum pox virus (PPV) is the most harmful disease of stone fruits in Europe and elsewhere world. There no highly PPV resistant cultivar plum. Biotech approach has led to development resistance through genetic engineering. In this study, we evaluated a transgenic plum Prunus domestica L., clone C5 (cv. HoneySweet), where based on RNA interference (RNAi). Resistance plums been for PPV, Prune dwarf (PDV), Apple chlorotic leaf spot (ACLSV) regulated field trial Czech Republic 12...
High-throughput sequencing technologies were used to identify plant viruses in cereal samples surveyed from 2012 2017. Fifteen genome sequences of a tenuivirus infecting wheat, oats, and spelt Estonia, Norway, Sweden identified characterized by their distances other sequences. Like most tenuiviruses, the this contains four genomic segments. The isolates found different countries shared at least 92% nucleotide sequence identity level. planthopper Javesella pellucida was as vector virus....
The bacterial communities in the gut of an insect have important ecological and functional effects on insect. However, community composition diversity microbiota insects that vector plant viruses are poorly understood. As vector, Psammotettix alienus transmits various including wheat dwarf virus (WDV). Here, we used combination leafhopper WDV as model to survey influence transmission characteristic vice versa. We characterized 22 phyla 249 genera all populations collected from six geographic...
Turnip yellows virus (TuYV) is one of the most important pathogens oilseed rape worldwide. The has a large host range including many crop species (e.g., rape, pea, chickpea) and weeds from more than twenty plant families. Other we detected TuYV in commonly grown weed that share fields vegetation period together with canola crops Czech Slovak Republics. was by reverse-transcription polymerase chain reaction (RT-PCR) at least 26 main hosts (oilseed rape), intercrops such as Amaranthus...
The tritimovirus Wheat streak mosaic virus (WSMV) is widespread throughout the world and represents a severe threat to cereal crop production. To increase knowledge of genetic diversity WSMV in Europe, until now scarce, capsid protein (CP) sequences several Czech, French, Italian, Slovak, Turkish isolates have been determined. A multiple alignment CP nucleotide using available revealed only limited sequence variation among 3 previously sequenced European 14 this study. Moreover, these were...
Background Although many genetic manipulations of crops providing biofortified or safer food have been done, the acceptance biotechnology still remains limited. We report on a transgenic barley expressing multi-functional protein osmotin that improves plant defense under stress conditions. Methods An Agrobacterium–mediated technique was used to transform immature embryos spring cultivar Golden Promise. Transgenic plants T0 and T1 generation were evaluated by molecular methods. tolerance...
Wheat dwarf virus (WDV) is one of the most important pathogens cereal crops worldwide. To understand molecular mechanism resistance, here we investigated comparative transcriptome wheat genotypes with different levels resistance (Svitava and Fengyou 3) susceptibility (Akteur) to WDV. We found a significantly higher number differentially expressed transcripts (DETs) in susceptible genotype than resistant (e.g., Svitava). The downregulated was also (Svitava) opposite true for upregulated...