A5052635659- Lysosomal Storage Disorders Research
- Prostate Cancer Treatment and Research
- Trypanosoma species research and implications
- Cellular transport and secretion
- Vibrio bacteria research studies
- Carbohydrate Chemistry and Synthesis
- Prostate Cancer Diagnosis and Treatment
- HIV Research and Treatment
- Escherichia coli research studies
- HIV/AIDS drug development and treatment
- Cancer, Lipids, and Metabolism
- Glycosylation and Glycoproteins Research
- Molecular Biology Techniques and Applications
- Cutaneous Melanoma Detection and Management
- Melanoma and MAPK Pathways
- Urologic and reproductive health conditions
- Glycogen Storage Diseases and Myoclonus
- Research on Leishmaniasis Studies
- Protease and Inhibitor Mechanisms
- T-cell and Retrovirus Studies
- Invertebrate Immune Response Mechanisms
- Immune Cell Function and Interaction
- Hippo pathway signaling and YAP/TAZ
- DNA Repair Mechanisms
- Advanced Biosensing Techniques and Applications
University of South Australia
2021-2024
South Australian Health and Medical Research Institute
2013-2023
South Australia Pathology
1993-2010
Women's and Children's Hospital
1996-2010
Women's and Children's Health Network
2006-2007
The University of Adelaide
1992-2005
Boston Children's Hospital
1997
Australian Centre for HIV and Hepatitis Virology Research
1993-1995
Plant Industry
1994
Commonwealth Scientific and Industrial Research Organisation
1994
Vibrio cholerae O1 exists as two major serotypes, Inaba and Ogawa, which are associated with the O antigen of lipopolysaccharide capable unequal reciprocal interconversion. The 20-kilobase rfb regions encoding O-antigen biosynthesis in strains 569B (Inaba) O17 (Ogawa) have been cloned Escherichia coli K-12 nucleotide sequences determined. Besides several base substitutions a small deletion sequence relative to O17, there is single change resulting TGA stop codon within gene for 32-kDa RfbT...
In this study, we have characterized the HIV DNA-containing replication complexes present in cells early after cell-to-cell infection, using sucrose gradient sedimentation and immunoprecipitation. Six hours a cytoplasmic complex sedimented as large structure (320S). This was precipitated by antisera to three virus-coded enzymes (reverse transcriptase, integrase, protease), matrix protein (p17), cellular histones but not major capsid (p24). associated with cell membranes could be dissociated...
Mucopolysaccharidosis type VI (MPS VI; Maroteaux-Lamy syndrome) is a lysosomal storage disorder caused by mutations in the N-acetylgalactosamine-4-sulfatase (arylsulfatase B, ARSB) gene. ARSB enzyme involved degradation of glycosaminoglycans (GAG) dermatan and chondroitin sulfate. reduce function GAG degradation, causing urinary excretion these partially degraded substrates. Disease onset rate progression variable, producing spectrum clinical presentation. In this study, 105 MPS...
The highly heterogenous nature of colorectal cancer can significantly hinder its early and accurate diagnosis, eventually contributing to high mortality rates. adenoma-carcinoma sequence serrated polyp-carcinoma are the two most common sequences in sporadic cancer. Genetic alterations adenomatous polyposis coli (APC), v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) tumour protein 53 (TP53) genes critical sequence, whereas v-Raf murine B (BRAF) MutL Homolog1 (MLH1) driving...
Early diagnosis of lysosomal storage disorders (LSDs), before the onset irreversible pathologies, will be a key factor in development effective therapies for many these disorders. Newborn screening offers potential mechanism early detection From studies both normal and LSD-affected human skin fibroblasts we identified lysosome-associated membrane protein LAMP-1 as diagnostic marker. We have developed sensitive method quantification this with time-resolved fluorescence immunoassay. A soluble...
The recent emergence of a pathogenic new non-O1 serotype (O139) Vibrio cholerae has led to numerous studies in an attempt identify the origins this strain. Our indicate that O139 strains have clear differences surface polysaccharides when compared with O1 strains: lipopolysaccharide can be described as semi-rough. Southern hybridization rfb region demonstrates no longer contain any genes required for synthesis O-antigen or its modification and also lack at least 6 kb additional contiguous...
Diagnosis and assessment of patients with prostate cancer is dependent on accurate interpretation grading histopathology. However, morphology does not necessarily reflect the complex biological changes occurring in disease progression, current biomarkers have demonstrated limited clinical utility patient assessment. This study aimed to develop that accurately define biology by distinguishing specific pathological features enable reliable pathology for Gleason patients. Online gene expression...
Abstract Prostate cancer (PCa) development and progression relies on the programming of glucose lipid metabolism, this involves alterations in androgen receptor expression signalling. Defining molecular mechanism that underpins metabolic will have direct significance for patients with PCa who a poor prognosis. Here we show there is dynamic balance between sortilin syndecan-1, reports different phenotypes. Using tissue microarrays, demonstrated by immunohistochemistry was highly expressed...
Sanfilippo A syndrome (MPS-IIIA) is a mucopolysaccharide lysosomal storage disorder caused by deficiency in the enzyme, sulphamidase (EC 3.10.1.1), which required for degradation of heparan sulphate. genomic clone containing entire gene was isolated from chromosome 17-specific gridded cosmid library. The structure and sequence exon/intron boundaries 5' promoter region were determined. split into 8 exons spanning approximately 11 kb.
Abstract The diagnosis of prostate cancer using histopathology is reliant on the accurate interpretation tissue sections. Current standards rely assessment Haematoxylin and Eosin (H&E) staining, which can be difficult to interpret introduce inter-observer variability. Here, we present a digital pathology atlas online resource micrographs for both H&E reinterpretation samples novel set three biomarkers as an interactive tool, where clinicians scientists explore high resolution from...
Gleason scoring is used within a five-tier risk stratification system to guide therapeutic decisions for patients with prostate cancer. This study aimed compare the predictive performance of routine H&E or biomarker-assisted ISUP (International Society Urological Pathology) grade grouping assessing biochemical recurrence (BCR) and clinical (CR) in retrospective was an assessment 114 men cancer who provided radical prostatectomy samples Australian Prostate Cancer Bioresource between 2006...
The presence of intraductal carcinoma the prostate (IDCP) correlates with late-stage disease and poor outcomes for patients prostatic adenocarcinoma, but accurate reliable staging severity remains challenging. Immunohistochemistry (IHC) has been utilised to overcome problems in assessing IDCP morphology, current markers have only demonstrated limited utility characterising complex biology this lesion. In a retrospective study cohort who had diagnosed IDCP, we IHC on radical prostatectomy...
Mucopolysaccharidosis type VI (MPS VI), or Maroteaux-Lamy syndrome, is a lysosomal storage disorder caused by deficiency of N-acetylgalactosamine-4-sulfatase (ARSB). Seven MPS patients were chosen for the initial clinical trial enzyme replacement therapy. Direct sequencing genomic DNA from these was used to identify ARSB mutations. Each individual exon gene amplified PCR and subsequently sequenced. Nine substitutions (c.289C>T [p.Q97X], c.629A>G [p.Y210C], c.707T>C [p.L236P], c.936G>T...
The premature stop codon mutations, Q70X and W402X, are the most common α-L-iduronidase gene (IDUA) mutations in mucopolysaccharidosis type I (MPS I) patients. Read-through drugs have been used to suppress codons, this can potentially be treat patients who of mutation. We examined effects aminoglycoside treatment on IDUA W402X cultured cells show that 4,5-disubstituted aminoglycosides induced more read-through for mutation, while 4,6-disubstituted promoted mutation: lividomycin...