A5103191858- Receptor Mechanisms and Signaling
- Microfluidic and Capillary Electrophoresis Applications
- Cardiomyopathy and Myosin Studies
- Muscle metabolism and nutrition
- Biochemical Acid Research Studies
- Neuropeptides and Animal Physiology
- Metabolism and Genetic Disorders
- Metabolomics and Mass Spectrometry Studies
- Vitamin C and Antioxidants Research
- Pharmacological Effects and Assays
- Muscle Physiology and Disorders
- Vitamin K Research Studies
- Microfluidic and Bio-sensing Technologies
- Muscle activation and electromyography studies
- Advanced MRI Techniques and Applications
- Renin-Angiotensin System Studies
- Nanopore and Nanochannel Transport Studies
- Cancer, Hypoxia, and Metabolism
- Renal function and acid-base balance
- Mass Spectrometry Techniques and Applications
- Lipid Membrane Structure and Behavior
- Neuroscience and Neural Engineering
- Ion channel regulation and function
- Biotin and Related Studies
- Nicotinic Acetylcholine Receptors Study
Michigan State University
2005-2020
University of Virginia
1981-2020
Michigan United
2010
Dillon Consulting
1987
The University of Texas at Austin
1986
Harvard University
1986
Brigham and Women's Hospital
1986
Fox Chase Cancer Center
1986
Phosphorylation of the 20,000-dalton light chain myosin is closely correlated with cross-bridge cycling in arterial smooth muscle. Evidence presented that dephosphorylation can produce an attached, noncycling (latch-bridge) which responsible for high economy force maintenance this tissue.
31P NMR spectra of isolated rabbit bladder and uterus were obtained under steady-state arterial perfusion in vitro at rest while stimulated. The contained seven major peaks: phosphoethanolamine, sn-glycero(3)phosphocholine, inorganic phosphate (Pi), phosphocreatine, the gamma, alpha, beta peaks ATP. Chemical analyses, high-pressure liquid chromatography, spectroscopy aqueous extracts bladders identified a number other components that also made contributions to, but not resolved in, intact...
Insulin (INS) resistance associated with hyperestrogenemias occurs in gestational diabetes mellitus, polycystic ovary syndrome, ovarian hyperstimulation estrogen therapies, metabolic and obesity. The mechanism by which INS interact is unknown. We hypothesize that binds directly to the insulin receptor (IR) producing resistance.To determine binding constants of steroid hormones INS, IR, INS-like peptides derived from IR; investigate effect estrogens on its receptor.Ultraviolet spectroscopy,...
Abstract Background Ladiratuzumab vedotin (LV) is an investigational anti-LIV-1 antibody-drug conjugate with a protease-cleavable linker to monomethyl auristatin E (MMAE). LIV-1 highly expressed in metastatic triple negative breast cancer (TNBC). LV mediated delivery of MMAE drives antitumor activity through cytotoxic cell killing and has been shown induce immunogenic death (ICD). monotherapy demonstrated encouraging TNBC. Anti-PD-(L)1 agents are emerging as part the TNBC treatment paradigm...
Ascorbate reduces the oxidation rate of catecholamines and, by an independent mechanism, enhances rabbit aortic ring contractions initiated catecholamines. The largest significantly different fractional increases in force produced ascorbate enhancement norepinephrine (NE), epinephrine, phenylpropanolamine (PPA), and ephedrine (Eph) are 5.5, 1.8, 1.6, 1.3 times, respectively. In physiological salt solutions bubbled with 95% O 2 at 37°C, NE, PPA, Eph have constants 1.24, 247, 643 h, 100 nM NE...
31P-NMR has been used extensively to estimate intracellular pH. It also can be measure extracellular pH and volume when an NMR-detectable phosphorous probe is used. Phosphonic acids have suggested as useful markers. The present study was designed assess the utility of phenylphosphonic acid (PPA) a marker in perfused smooth muscle. Rabbit bladder strips were exposed PPA concentrations 1-20 mM. Tension development response maximal carbachol challenges (10 microM) independent concentration....
Rationale Previous in vitro research demonstrated that ascorbate enhances potency and duration of activity agonists binding to alpha 1 adrenergic histamine receptors. Objectives Extending this work beta 2 systems vivo. Methods Ultraviolet spectroscopy was used study receptor preparations peptides. Force transduction studies on acetylcholine-contracted trachealis from pigs guinea measured the effect relaxation due submaximal doses agonists. The inhaled albuterol with without tested horses...
A method for the measurement of tissue metabolites from rabbit urinary bladder using capillary electrophoresis (CE) has been developed. The generates a reproducible electropherogram containing >20 peaks, including NAD, NADH, lactate, UDP-glucose, phosphocreatine, creatine, ATP, ADP, GTP, and UTP, <20 nl extract solution generated 1.1 (or ∼1.2 μg) in <40 min. Multiple samples same produce SE comparable with enzymatic or nuclear magnetic resonance (NMR) measurements metabolites:...
This study tested the hypothesis that adenosine released from isolated guinea pig hearts (n = 5) in response to norepinephrine is related cellular phosphorylation potential (PP; [ATP]/[ADP][Pi]), where Pi inorganic phosphate. 31P-nuclear magnetic resonance (NMR) was used measure relative concentrations of Pi, phosphocreatine (PCr), and ATP. Hearts were Langendorff perfused with a physiological salt solution containing 0.1 mM Pi. The venous effluent collected for measurement partial pressure...
Abstract 31 ‐NMR spectroscopy was performed on vascular smooth muscle (VSM; porcine carotid artery) superfused with a substrate‐free high K + ‐PSS. Scans were collected before (control), during (hypoxia), and after (post‐control) hypoxia, chemical measurements of ATP (0.070 ± 0.13 m̈moles/g wet wt.) creatine (2.04 0.14 m̈mol/g made. During well‐defined beta‐ADP signals consistently resolved. Their areas indicated that 30, 60, 90 min free ADP 0.05 0.01, 0.09 0.12 0.01 wt., respectively. The...
Ascorbate has previously been shown to enhance both alpha(1)- and beta(2)-adrenergic activity. This activity is mediated by ascorbate binding the extracellular domain of adrenergic receptor, which also decreases oxidation rate ascorbate. H1 histamine receptors have agonist or sites with strong similarities alpha(1-) receptors. Physiological concentrations (50 microM) significantly enhanced contractions rabbit aorta on lower half dose-response curve, increasing 0.1, 0.2, 0.3 microM two-...
The intracellular recycling of ascorbic acid from dehydroascorbic by the glutathione-glutathione reductase system has been well-characterized. We propose that extracellular is performed in a similar manner cysteine-rich, glutathione-like regions first and second loops some aminergic receptors including adrenergic, histaminergic, dopaminergic receptors. Previous research our laboratory demonstrated binds to these at site on their or loops, significantly enhancing ligand activity, apparently...
Several classes of compounds that have no intrinsic activity on aminergic systems nonetheless enhance the potency receptor ligands three-fold or more while significantly increasing their duration activity, preventing tachyphylaxis and reversing fade. Enhancer include ascorbic acid, ethylenediaminetetraacetic corticosteroids, opioid peptides, opiates opiate antagonists. This paper provides first review enhancement, demonstrating all enhancers a common, inobvious molecular motif work through...
Magnetization exchange experiments and force analysis were performed on porcine carotid arteries with varied phosphocreatine (PCr) levels. The aim of these was to determine the creatine kinase (CK) kinetics role in hypoxic relaxation.The magnetization techniques used multisite saturation transfer (MST) conventional (CST). two because CST assumes a two-site while MST allows one assume three-site exchange. Mechanical parameters tension generation relaxation measured energetic effects...
The seed moisture level marking the onset of imbibitional injury (breakpoint) was determined for two cultivars Phaseolus vulgaris L. cvs ;Tendercrop' (TC) and ;Kinghorn Wax' (KW). At 20 degrees C breakpoints were 0.15 gram H(2)O/gram dry weight (gram per gram) TC 0.11 KW. When seeds imbibed at 5 C, 0.19 0.16 Below breakpoint germination changed 4.6%/0.01 all treatments. Imbibition rates maximal 0.07 0.33 after minutes imbibition. Rates electrolyte leakage correlated with imbibition rate...
Abstract Ascorbic acid (vitamin C) enhances the smooth muscle and cardiac activity of norepinephrine, epinephrine, other adrenergic drugs, increasing dose response up to threefold duration fivefold. The effect is not due antioxidant effects ascorbate. mechanism by which this enhancement occurs as yet unknown. This discovery has broad implications for utilizing all existing drugs well formulating new mixtures delivery such drugs. Despite clear‐cut pharmaceutical potential discovery, it was...