A5101418092- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
- Machine Learning in Bioinformatics
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Escherichia coli research studies
- Immune Response and Inflammation
- vaccines and immunoinformatics approaches
- Gut microbiota and health
- Antimicrobial Peptides and Activities
- Antibiotic Resistance in Bacteria
- Bioinformatics and Genomic Networks
- Aquaculture disease management and microbiota
- NF-κB Signaling Pathways
- Cancer-related Molecular Pathways
- Hepatitis C virus research
- Neonatal Health and Biochemistry
- Methemoglobinemia and Tumor Lysis Syndrome
- Computational Drug Discovery Methods
- Hemoglobinopathies and Related Disorders
- Biochemical and Molecular Research
- Cancer Research and Treatments
- Enzyme Structure and Function
National Institute of Cholera and Enteric Diseases
2016-2025
National Institute of Occupational Health
2022-2025
Indian Council of Medical Research
2024
Translational Research Informatics Center (Japan)
2011
Tufts Medical Center
2005
Molecular Oncology (United States)
2005
Thomas Jefferson University
2002
Sidney Kimmel Cancer Center
2002
Macrophages and B-cells from Tpl2 knock-out mice exhibit a restricted defect in lipopolysaccharide death receptor signaling that is limited to the activation of ERK. Here we show Tpl2-/- MEFs defects ERK, JNK, NF-kappaB activation, or ERK only when stimulated with tumor necrosis factor-alpha (TNF-alpha) interleukin-1beta, respectively. In addition, by TNF-alpha depends on signals transduced both TRAF2 RIP1. Activated phosphorylates MKK4/SEK1 upstream JNK stimulates DNA binding...
Cathelicidin (hCAP-18/LL-37) and beta-defensin 1 (HBD-1) are human antimicrobial peptides (AMPs) with high basal expression levels, which form the first line of host defence against infections over epithelial surfaces. The functions owe to their direct microbicidal effects as well immunomodulatory role. Pathogenic microorganisms have developed multiple modalities including transcriptional repression combat this arm immune response. precise mechanisms pathogen-derived molecules responsible...
Viral-host protein-protein interaction plays a vital role in pathogenesis, since it defines viral infection of the host and regulation proteins. Identification key viral-host interactions (PPIs) has great implication for therapeutics.In this study, systematic attempt been made to predict PPIs by integrating different features, including domain-domain association, network topology sequence information using from VirusMINT. The three well-known supervised machine learning methods, such as SVM,...
This study identifies and characterizes a gold nano-particle tagged antimicrobial peptide that can internalize cells kill intracellular bacteria without being cytotoxic. It also considerably reduce<italic>S.</italic>Typhi infection in animal model.
More than half of all Salmonella enterica serovar Typhi genes still remain unannotated. Although pathogenesis S . is incompletely understood, treatment typhoid fever complicated by the emergence drug resistance. Effectiveness currently available vaccines also limited. In search novel virulence proteins, we have identified several putative adhesins through computational approaches. Our experiment shows that a 27-kDa outer membrane protein (T2544) plays major role in bacterial adhesion to host...
Human
Introduction Screening for G6PD deficiency can inform disease management including malaria. Treatment with the antimalarial drugs primaquine and tafenoquine be guided by point-of-care testing deficiency. Methods findings Data from similar clinical studies evaluating performance of STANDARD Test (SD Biosensor, South Korea) conducted in Bangladesh, Brazil, Ethiopia, India, Thailand, United Kingdom, States were pooled. was assessed a retrospective analysis on capillary venous specimens. All...
Little is known about the regulation of innate host defense peptide cathelicidin at mucosal surfaces. Expression believed to be transcriptionally regulated, and several cis-acting elements have been identified in putative promoter. However, trans-acting factors not clearly defined. We recently reported that bacterial exotoxins suppress expression sodium butyrate-differentiated intestinal epithelial cells (ECs), this may mediated through inducible cAMP early repressor. Here we shown...
Abstract Background With the global spread of multidrug resistance in pathogenic microbes, infectious diseases emerge as a key public health concern recent time. Identification host genes associated with will improve our understanding about mechanisms behind their development and help to identify novel therapeutic targets. Results We developed machine learning techniques-based classification approach disease-associated by integrating sequence protein interaction network features. Among...
Toll-like receptor 5 (TLR5) expression in the intestinal epithelial cells (IECs) is critical to maintain health, as underscored by multiple and extra-intestinal diseases mice genetically engineered for IEC-specific TLR5 knockout. A gradient of exists colonic from cecum distal colon. Intriguingly, an identical dietary metabolite, butyrate also luminal contents. However, both being homeostasis immune response, no studies examined role regulation expression. We showed that transcriptionally...
Intracellular pathogens of Salmonella spp. survive and replicate within the phagosomes, called Salmonella-containing vacuoles (SCVs) inside macrophage by manipulating phagosomal maturation phagolysosome formation. While controversies exist about traffic Typhimurium, little studies were carried out with intracellular survival mechanisms Typhi ( S. Typhi). We had previously reported that a eukaryote-like serine/threonine kinase (T4519) contributes to macrophages activates host pro-inflammatory...
Small RNAs (sRNAs) in bacteria have emerged as key players transcriptional and post-transcriptional regulation of gene expression. Here, we present a statistical analysis different sequence- structure-related features bacterial sRNAs to identify the descriptors that could discriminate from other RNAs. We investigated comprehensive heterogeneous collection 816 sRNAs, identified by northern blotting across 33 species compared their various with classes RNAs, such tRNAs, rRNAs mRNAs. observed...
Abstract Targeting bacterial virulence mechanisms without compromising growth is a promising strategy to prevent drug resistance. LysR-type transcriptional regulators (LTTRs) possess structural conservation across species and regulate in numerous pathogens, making them attractive targets for antimicrobial agents. We targeted AphB, Vibrio cholerae LTTR, which regulates the expression of genes encoding cholera toxin toxin-co-regulated pilus inhibitor designing. Since AphB ligand unknown, we...
Cardioprotective potential of anthocyanin rich red cabbage extract (ARCE) was assessed in H2O2 treated rat neonatal cardiomyoblasts (H9c2 cells) and isoproterenol (ISO) induced rodent model myocardial infarction. H9c2 cells recorded cytotoxicity (48–50%) apoptosis (57.3%), the same were reduced presence ARCE (7–10% & 12.3% respectively). Rats pretreated with for 30 days followed by ISO treatment favourable heart: body weight ratio as compared to group. Also, mRNA levels enzymatic...
How Salmonella enterica serovar Typhi (S. Typhi), an important human pathogen, survives the stressful microenvironments inside gastrointestinal tract and within macrophages remains poorly understood. We report here that S. has a bonafide stringent response (SR) system, which is mediated by (p)ppGpp regulates multiple virulence-associated traits pathogenicity of Ty2 strain. In iron overload mouse model infection, (p)ppGpp0 (Ty2ΔRelAΔSpoT) strain showed minimal systemic spread no mortality, as...
Abstract Bacterial small non-coding RNAs (sRNAs) are not translated into proteins, but act as functional RNAs. They involved in diverse biological processes like virulence, stress response and quorum sensing. Several high-throughput techniques have enabled identification of sRNAs bacteria, experimental detection remains a challenge grossly incomplete for most species. Thus, there is need to develop computational tools predict bacterial sRNAs. Here, we propose method identify bacteria using...
Helicobacter pylori is a gastric mucosal pathogen and associated with diseases like peptic ulcer cancer. To combat H. infection, there an urgent need for new class of antibiotics due to the emergence drug-resistant strains. Enzymes involved in bacterial lysine biosynthetic pathways may be potential targets antibacterial drug development, since essential component peptidoglycan cell wall. No pathway exists biosynthesis humans; hence, inhibitors targeting enzymes have selective toxicity....
We present here in silico studies on antiviral drug resistance due to a novel mutation of influenza A/H1N1 neuraminidase (NA) protein. Influenza virus was responsible for recent pandemic and is currently circulating among the seasonal strains. M2 NA are two major viral proteins related pathogenesis humans have been targeted designing. Among them, preferred because ligand-binding site highly conserved between different strains virus. Different mutations active residues leading or...
Abstract Development of safe, highly effective and affordable enteric fever vaccines is a global health priority. Live, oral typhoid induce strong mucosal immunity long-term protection, but safety remains concern. In contrast, efficacy wears off rapidly for injectable, polysaccharide-based vaccines, which elicit poor response. We previously reported Salmonella Typhi outer membrane protein, T2544 as potential candidate bivalent ( S . Paratyphi A) vaccine development. Here, we show that...