A5048654962- Asthma and respiratory diseases
- Mast cells and histamine
- Neuropeptides and Animal Physiology
- Respiratory and Cough-Related Research
- Eosinophilic Disorders and Syndromes
- Immune Response and Inflammation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Nitric Oxide and Endothelin Effects
- Eicosanoids and Hypertension Pharmacology
- Inhalation and Respiratory Drug Delivery
- Drug-Induced Adverse Reactions
- Food Allergy and Anaphylaxis Research
- Urticaria and Related Conditions
- Pleural and Pulmonary Diseases
- Allergic Rhinitis and Sensitization
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Contact Dermatitis and Allergies
- Receptor Mechanisms and Signaling
- Pharmacology and Obesity Treatment
- Pain Mechanisms and Treatments
- Occupational exposure and asthma
- Adenosine and Purinergic Signaling
- Leprosy Research and Treatment
- IL-33, ST2, and ILC Pathways
- Polyamine Metabolism and Applications
Fundação Oswaldo Cruz
2005-2022
Universidade Federal do Rio de Janeiro
2015
Marymount University
2012
Northwestern University
2012
Williams & Associates
2012
Universidade do Estado do Rio de Janeiro
1999
Imperial College London
1999
Institut Pasteur
1987-1997
National Institute of Science and Technology of Drugs and Medicines
1997
Inserm
1987
Abstract In noninfected rats, challenge with allergen following local IgE sensitization induced a pleurisy marked by intense protein exudation that plateaued from 30 min to 4 h after challenge, reducing thereafter. Infection of rats Angiostrongylus costaricensis 5-fold increase in blood eosinophil numbers 25 days postinfection, whereas the eosinophils pleural cavity ranged normal weak increase. infected identically sensitized, Ag much shorter duration edema complete resolution h, but no...
Tissue eosinophilia prevention represents one of the primary targets to new anti-allergic therapies. As lipoxin A4 (LXA4) and aspirin-triggered 15-epi-LXA4 (ATL) are emerging as endogenous "stop signals" produced in distinct pathologies including some eosinophil-related pulmonary disorders, we evaluated impact situ LXA4/ATL metabolically stable analogues on allergen-induced eosinophilic pleurisy sensitized rats. dramatically blocked allergic pleural eosinophil influx, while concurrently...
Evidence suggests that nebulized lidocaine is beneficial in asthma therapy, but to what extent and the mechanisms underlying this effect remain poorly understood. The aim of study was assess impact treatment using a murine model allergic characterized by expression pivotal features disease: inflammation, mucus production, lung remodeling.A/J mice sensitized with ovalbumin were treated inhaled or vehicle immediately after intranasal challenges. Lung function, total differential leukocytes...
The injection of 100 or 300 μg carrageenin into the mouse paw pleural cavity produced a delayed inflammatory reaction at 48 h while platelet activating factor (PAF)‐induced oedema and pleurisy were maximal 30 min after its injection. PAF antagonist, WEB 2086, failed to inhibit induced by PAF, but reduced first phase (1–4 h) without interfering with second one (48–72 h). In contrast, another structurally‐related 2170, inhibited dose‐dependently both (48 Repeated injections led significant...
The intrapleural injection of Paf‐acether into rats caused, at 30 min, a marked exudation accompanied by reduction in the pleural leucocyte count. At 6 h, exudate volume had decreased and significant increase total count, particularly eosinophils was noted. Two antagonists, WEB 2086 48740 RP abrogated leucopenia observed min after administration, whereas inhibited only former. Pleurisy also reduced about 60% with dexamethasone, 45% BW 755C or LY 171883, mixed cyclo‐oxygenase/lipoxygenase...
Prior studies have emphasized the anti-inflammatory and antioxidant properties of polyisoprenylated benzophenone derivatives, but their putative effect on allergic conditions has not yet been addressed. In current study, naturally occurring 7-epiclusianone, isolated from Garcinia brasiliensis, was investigated to check its effectiveness allergen-evoked intestinal spasm. The standard antiallergic azelastine used for comparison. We found that 7-epiclusianone inhibited antigen-induced...
Inhalation of the local anaesthetic lidocaine has been suggested to be beneficial for asthmatics, but airway anaesthesia is unpleasant and may exacerbate bronchoconstriction. Our previous study showed that inhalation analogue JMF2-1 can elicit anti-inflammatory properties without anaesthesia. This prompted further research on mechanism action putative therapeutic application JMF2-1.We tested hypothesis would prevent allergen-induced lung inflammation hyperresponsiveness (AHR) by modulating T...
Gold nanoparticles (AuNPs) can inhibit pivotal pathological changes in experimental asthma, but their effect on steroid-insensitive asthma is unclear. The current study assessed the effectiveness of nebulized AuNPs a murine model glucocorticoid (GC)-resistant asthma.A/J mice were sensitized and subjected to intranasal instillations ovalbumin (OVA) once week for nine weeks. Two weeks after starting allergen stimulations, Budesonide or AuNP nebulization 1 h before stimuli. Analyses carried out...
The pharmacological profile of a novel specific platelet-activating factor (PAF) receptor antagonist - yangambin isolated from the Brazilian plant Ocotea duckei Vattimo (Lauraceae), was investigated in pentobarbitone-anaesthetized rabbit. i.v. administration PAF (0.03 3.0 µg kg-1) induced marked but reversible hypotensive effects and mild reductions heart rate. Both are independent respiratory conditions imposed on animals. Moreover, (3.0 kg-1, i.v.) decrease circulating levels platelets...
An aqueous crude extract and a polar fraction derived from this were prepared leaves of Vernonia condensata Baker assessed in standard rodent models algesia ulcerogenesis. Oral pretreatment with the lyophilized (200 mg/kg) significantly reduced mouse writhing counts caused by i.p. injection increasing concentrations (0.6-1.2%) acetic acid (0.1 ml/10 g). In doses ranging 50 to 400 mg/kg, inhibited dose-dependently induced (0.6%) (ED50 = 241 also markedly increased sleeping time thiopental....
Bradykinin is suggested to play a role in the pathophysiology of several acute and chronic diseases, including allergic disorders such as asthma. In present study, we have investigated importance bradykinin mediating inflammation rats. To this end tested effects B 2 receptor antagonists Hoe 140, FR173657 or FR172357 on pleural inflammatory response triggered by intrapleural (i.pl.) injection allergen (ovalbumin, 12 μg cavity −1 ) 14 day‐actively sensitized Wistar Analysis fluid effluent...
The effects of the furofuran lignan yangambin on rabbit platelet aggregation and binding [3 H]-PAF to plasma membranes were studied. Log concentration-response curves PAF obtained in presence or absence increasing concentrations yangambin. This dose-dependently inhibited PAF-induced platelet-rich (PRP) shifted right without decreasing maximal response. Schild plot constructed from these data showed a slope 1.17 pA2 6.45. Moreover, at 10-5 M did not inhibit induced by ADP (5 × 10-7 M),...
1 The current study analyses the effects of endothelin-1 (ET-1) on paw oedema and pleurisy induced by platelet activating factor (PAF) other inflammatory agents in mouse. 2 Combined subplantar injection ET-1 (0.5 pmol/paw) did not modify caused histamine (1 to 100 μmol/paw), 5-hydroxytryptamine μmol/paw) or bradykinin nmol/paw) but markedly inhibited response PAF (0.95 3.8 nmol/paw). selective action against PAF-induced (1.9 was dose-dependent, reaching a maximum at 0.5 pmol/paw lasted up h....
The importance of developing new animal models to assess the pathogenesis glucocorticoid (GC)-insensitive asthma has been stressed. Because asthma-prone background A/J mice, we hypothesized that changes in these animals would be or become resistant GCs under repeated exposures an allergen. mice were challenged with OVA for 2 4 consecutive d, starting on day 19 postsensitization. Oral dexamethasone inhaled budesonide given 1 h before challenge, and analyses done 24 after last challenge....
Abstract Glucagon has been shown to be beneficial as a treatment for bronchospasm in asthmatics. Here, we investigate if glucagon would prevent airway hyperreactivity (AHR), lung inflammation, and remodeling murine model of asthma. (10 100 µg/Kg, i.n.) significantly prevented AHR eosinophilia BAL peribronchiolar region induced by ovalbumin (OVA) challenge, while only the dose µg/Kg inhibited subepithelial fibrosis T lymphocytes accumulation lung. The inhibitory action occurred parallel with...
In this study we examined the ability of furofuran lignan yangambin to influence local and systemic responses induced by antigen or PAF in actively sensitized normal rats. Given intraperitoneally 1 h before stimulation, inhibited pleural neutrophil eosinophil infiltration evoked i.pl. injection into 14 day-sensitized rats whereas plasma exudation both stimuli was unaffected. The influx (6 h) after LTB4 stimulation also significantly yangambin. We evidenced that hemoconcentration,...
Abstract In this work we characterize the Mycobacterium bovis bacillus Calmette-Guérin(BCC) -induced pleurisy and investigate role of chemical mediators cytokines in BCG-induced granulocyte accumulation at 24 h. Intrathoracic injection BCG C57B1/6 mice induces a biphasic inflammatory reaction with intense leukocyte h 15 days. Neutrophils were observed pleural cavity 4-24 h, mononuclear cells eosinophils after A new wave neutrophils Pretreatments dexamethasone, BW 755C, A4C, WEB 2170, L-NAME,...