A5038084939- Renin-Angiotensin System Studies
- Nitric Oxide and Endothelin Effects
- Receptor Mechanisms and Signaling
- Ion Transport and Channel Regulation
- Adenosine and Purinergic Signaling
- Chronic Kidney Disease and Diabetes
- Inflammatory mediators and NSAID effects
- Hormonal Regulation and Hypertension
- Eicosanoids and Hypertension Pharmacology
- Electrolyte and hormonal disorders
- Dialysis and Renal Disease Management
- Heart Failure Treatment and Management
- Complementary and Alternative Medicine Studies
- Ion channel regulation and function
- Renal and related cancers
- Organ Donation and Transplantation
- Renal function and acid-base balance
- Diabetes Treatment and Management
- Acute Kidney Injury Research
- Neuropeptides and Animal Physiology
- Renal Diseases and Glomerulopathies
- Neuroscience of respiration and sleep
- Acupuncture Treatment Research Studies
- Birth, Development, and Health
- Health Systems, Economic Evaluations, Quality of Life
University of Michigan
1992-2024
American Society of Nephrology
2020-2023
Cleveland Clinic
2023
Cleveland Clinic Lerner College of Medicine
2023
Case Western Reserve University
2023
Health Affairs
2020
National Center for Complementary and Integrative Health
2009-2020
Patient-Centered Outcomes Research Institute
2020
National Institutes of Health
2006-2017
Agency for Healthcare Research and Quality
2016
The incidence of kidney failure treatment in the United States increased 57% from 1991 to 2000. Chronic disease (CKD) prevalence was 11% among U.S. adults surveyed 1988 1994. objective this study estimate awareness CKD population during 1999 2000 and determine whether compared with Analysis conducted nationally representative samples noninstitutionalized adults, aged 20 yr older, two National Health Nutrition Examination Surveys 1994 (n = 15,488) 4101) for +/- SE. Awareness is self-reported....
The worldwide burden of kidney disease is rising, but public awareness remains limited, underscoring the need for more effective communication by stakeholders in health community. Despite this clarity, nomenclature describing function and lacks uniformity. In June 2019, Kidney Disease: Improving Global Outcomes (KDIGO) convened a Consensus Conference with goal standardizing refining used English language to describe disease, developing glossary that could be scientific publications. Guiding...
Adenosine is a determinant of metabolic control organ function increasing oxygen supply through the A2 class adenosine receptors and reducing demand A1 (A1AR). In kidney, activation A1AR in afferent glomerular arterioles has been suggested to contribute tubuloglomerular feedback (TGF), vasoconstriction elicited by elevations [NaCl] macula densa region nephron. To further elucidate role TGF, we have generated mice which entire coding sequence was deleted homologous recombination. Homozygous...
The present studies were undertaken to determine the effect of dietary salt intake on renal expression cyclooxygenase-1 (COX-1) and -2 (COX-2). Protein levels assessed by Western blotting, mRNA was reverse transcription-polymerase chain reaction (RT-PCR) cDNA prepared from kidney regions, dissected nephron segments, cultured cells. Both isoforms expressed at high in inner medulla (IM), with low detected outer cortex. COX-1 glomerulus all along collecting duct, whereas COX-2 restricted macula...
An in vitro method has been used to examine whether secretion of renin from the juxtaglomerular apparatus is affected by changes sodium chloride concentration tubular fluid at macula densa. Single apparatuses were microdissected rabbits and tubule segment containing densa was perfused, while simultaneously entire superfused, collected for measurement. In this preparation, which influences renal nerves local hemodynamic effects are eliminated, a decrease results prompt stimulation release rate.
Adenosine coordinates organ metabolism and blood supply, it modulates immune responses. In the kidney mediates vascular response elicited by changes in NaCl concentration macula densa region of nephron, thereby serving as an important regulator GFR. To determine whether adenosine formation depends on extracellular nucleotide hydrolysis, we studied NaCl-dependent GFR regulation (tubuloglomerular feedback) mice with targeted deletion ecto-5'-nucleotidase/CD73 (e-5'NT/CD73), enzyme responsible...
Transport inhibitor and ion substitution studies were performed using perfused, superfused preparations of the isolated rabbit juxtaglomerular apparatus to investigate transport dependency macula densa-mediated renin secretion. In first experimental series, tubular perfusion with a high-NaCl solution containing 10(-6) M bumetanide increased secretion compared high NaCl alone from 8.7 24.6 nano-Goldblatt hog units (nGU)/min. Bath addition had no effect on release. The second series tested...
We will address the strategic, organizational, and technical aspects that must be considered through an assessment of current landscape data available to clinicians patients for use in clinical decision making opportunities enhancing body evidence.2. work better identify describe ongoing activities contributing narrowing evidence gap approaches leverage extend volumes relevant digital health care facilitate efficient, streamlined randomized trials high-quality observational studies.3....
We are excited to present the abstracts of keynote speakers, parallel sessions and oral poster presentations World Congress on
The discovery that 15-deoxy-Δ 12,14 -prostaglandin J 2 (15d-PGJ ) is a ligand for the γ-isoform of peroxisome proliferator-activated receptor (PPAR) suggests nuclear signaling by prostaglandins. Studies were undertaken to determine nephron localization PPAR isoforms and their heterodimer partners, retinoid X receptors (RXR), evaluate function this system in kidney. PPARα mRNA, determined RT-PCR, was found predominately cortex further localized proximal convoluted tubule (PCT); PPARγ abundant...
Reducing luminal NaCl concentration in the macula densa region of nephron stimulates renin secretion, and this response is blocked by a specific inhibitor cyclooxygenase-2 (COX-2) (Traynor, T. R., Smart, A., Briggs, J. P., Schnermann, (1999) Am. Physiol. Renal 277, F706-710). To study whether low activates COX-2 activity or expression we clonally derived cell line (MMDD1 cells) from SV-40 transgenic mice using fluorescence-activated sorting renal tubular cells labeled with segment-specific...
Experiments were performed to examine the effect of changes in dietary salt intake on neuronal form constitutive nitric oxide synthase (ncNOS, type I NOS), renin, and angiotensinogen mRNA expression kidney. Three groups Sprague-Dawley rats studied as follows: maintained a 3% Na diet plus 0.45% NaCl drinking fluid for 7 days (high salt), given single injection furosemide (2 mg/kg i.p.) 0.03% (low containing 0.2% (control). was assessed with reverse transcription-polymerase chain reaction...
The present study was undertaken to investigate the mRNA localization of two major kidney-specific Na-K-Cl transport proteins, bumetanide-sensitive cotransporter (NKCC2 in rabbit and BSC1 rat) thiazide-sensitive (TSC). NKCC2 from mouse has been shown exist three isoforms (designated A, B, F) that differ only a 96-bp region. divergent region each cloned rat kidney by polymerase chain reaction (PCR)-based strategy, isoform-specific primers were chosen. RNA cDNA prepared renal cortex medulla...
Experiments were performed in anesthetized rats to study the effect of selective adenosine1 (A1) receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (CPX) on tubuloglomerular feedback (TGF) responses assessed as maximum change stop-flow pressure (PSF). Compared with control, PSF reduced during luminal application CPX at 10(-4) and 10(-5)M (-4.9 +/- 0.44 vs. + 0.9 0.42 mmHg -6.8 0.69 -1.4 0.7 mmHg, respectively), peritubular administration 10(-4)M (-6.2 -2.8 mmHg), infusion M into lumen a...
Previous results from our laboratory have shown that in the isolated perfused juxtaglomerular apparatus, nonselective inhibitors of cyclooxygenase (COX) activity prevent stimulation renin secretion by a reduction luminal NaCl concentration at macula densa. The present studies were performed to examine which COX isoform is involved NaCl-dependent secretion. In absence inhibitors, (from Na 141/Cl 120 mM 26/Cl 7 mM) caused an increase rate 4.5 +/- 1.8 26.1 7.4 nGU/min (P < 0.01, n = 19)....