Lacto-

Lacto-N-neotetraose (LNnT) and lacto-N-tetraose (LNT) are important oligosaccharides found in breast milk commonly used as nutritional supplements infant formula. We metabolic engineering techniques to optimize the modified Escherichia coli BL21 star (DE3) strain for efficient synthesis of LNnT LNT using β-1,4-galactosyltransferase (HpgalT) from Helicobacter pylori β-1,3-galactosyltransferase (SewbdO) Salmonella enterica subsp. salamae serovar, respectively. Further, we optimized expression...

2′-fucosyllactose (2′-FL), one of the simplest but most abundant oligosaccharides in human milk, has been demonstrated to have many positive benefits for healthy development newborns. However, high-cost production and limited availability restrict its widespread use infant nutrition further research on potential functions. In this study, basis previous achievements, we developed a powerful cell factory by using lacZ -mutant Escherichia coli C41 (DE3)ΔZ ulteriorly increase 2′-FL feeding...

Abstract We have obtained column density maps for an unbiased sample of 120 molecular clouds in the third quadrant Milky Way midplane ( b ≤ ∣5∣°) within Galactic longitude range from 195° to 225°, using high-sensitivity 12 CO and 13 J = 1 − 0) data Imaging Scroll Painting (MWISP) project. The probability functions hydrogen clouds, N-pdfs, are fitted with both a lognormal (LN) function plus power-law (LN+PL) function. classified into three categories according their shapes i.e., LN, LN+PL, UN...

We report the first high-sensitivity HI observation toward spiral galaxy M94 with Five-hundred-meter Aperture Spherical radio Telescope (FAST). From these observations, we discovered that has a very extended disk, twice larger than observed by THINGS, which is accompanied an filament and seven HVCs (high velocity clouds) at different distances. The projected distances of clouds are less 50 kpc from galactic center. measured total integrated flux (including all clouds/filament) 127.3 ($\pm$1)...

Abstract We universally search for evidence of kinematic and spatial correlation supernova remnant (SNR) molecular cloud (MC) associations nearly all SNRs in the coverage Milky Way Imaging Scroll Painting CO survey, i.e., 149 SNRs, 170 SNR candidates, 18 pure pulsar wind nebulae 1° < l 230° −5.°5 b 5.°5. Based on high-quality unbiased 12 CO/ 13 CO/C O ( J = 1–0) survey data, we apply automatic algorithms to identify broad lines correlations gas each region. The 91% SNR–MC detected...

Levilactobacillus brevis strains can be isolated from traditional Chinese pickles and used as the starter cultures to improve nutritional profiles of fermented juices. Three L. (LBG-29, LBG-24, LBD−14) that produce high levels gamma-aminobutyric acid (GABA; >300 mg/L) were pickles. The showed tolerance low pH bile salts exhibited safety in vitro. Litchi juice was using each strain at 37 °C for 48 h. litchi determined a good substrate fermentation process enhanced its functional profile....

The probiotic Weizmannia coagulans (W. coagulans) BC2000 can increase the abundance of intestinal transforming ellagic acid (EA) bacteria and inhibit metabolic disorders caused by hyperlipidemia activating liver autophagy. This study aimed to investigate inhibitory effects W. EA on hyperlipidemia-induced cholesterol metabolism disorders. C57BL/6J mice (n = 10 in each group) were fed a low-fat diet, high-fat diet (HFD), HFD supplemented with EA, BC77, BC2000. supplementation prevented...

3'-Sialyllactose (3'-SL), a key component of human milk oligosaccharides, provides significant health benefits and immune modulation, is increasingly used in infant formula dietary supplements. This study presents novel approach for the efficient biosynthesis 3'-SL using

N-acetylneuraminic acid (NANA) is a key ingredient in bird’s nest, as well health products and infant formulas. However, the absence of suitable reference materials (RMs) makes it challenging to ensure quality these products. Currently, NANA primarily extracted from but this process costly difficult scale up. In study, we successfully prepared large-scale yield RM fermentation products, which increases reduces its cost. IR, MS, NMR methods were used for qualitative analysis RM, HPLC was...

3-Fucosyllactose (3-FL), an essential component of human milk oligosaccharides, is crucial for infant health. However, the extraction 3-FL from presents a significant challenge. In this study, E. coli BL21 Star (DE3) was engineered biosynthesis, and gene combinations metabolic pathways were optimized to obtain high-yield production strain. First, module integrated into coli. Second, efficient alpha-1,3-fucosyltransferase screened. Then, different integration sites used optimize precursor...

This study presents an engineered strain of Escherichia coli specifically designed to enhance the production l-fucose while minimizing residues 2′-fucosyllactose. The optimization strategies employed include selection key enzymes, gene copy numbers, and fermentation using mixed carbon sources. metabolic flux was directed toward synthesis by integrating preferred 1,2-fucosyltransferase α-l-fucosidase into genome. Furthermore, numbers were optimized enzyme expression, thereby increasing...

Ectoine is an important natural macromolecule protector that helps extremophiles maintain cellular stability and function under high-salinity conditions. Recently, the development of microbial strains for high-level ectoine production has become attractive research direction. In this study, we constructed efficient plasmid-free ectoine-producing strain. We modified 5′-untranslated region ectABC gene cluster from Halomonas elongate to fine-tune expression genes ectA, ectB, ectC. Furthermore,...

Abstract We uncovered a more tilted molecular gas structure with highly negative velocities located near the dust lane. Our observations show that approaching flows from overshoot process are captured by gravitational potential of bar and then flow toward Galactic central zone (CMZ) through channel. The recycled effect, in conjunction freshly accreted inner 3 kpc disk, accumulates significantly <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" overflow="scroll"> <mml:msub> <mml:mrow>...

Three-dimensional (3D) and two-dimensional (2D) Ag-based zwitterionic metal-organic frameworks (MOFs) [Ag2(Cedcp)]n (1, 3D, H3CedcpBr denotes N-(carboxyethyl)-(3,5-dicarboxyl)-pyridinium bromide) {[Ag4(Cmdcp)2(H2O)4]·4H2O}n (2, 2D, H3CmdcpBr N-(carboxymethyl)-(3,5-dicarboxyl)-pyridinium have been prepared investigated for antimicrobial activity via minimal inhibition concentration (MIC) test killing kinetic assay. Both MOFs 1 2 show good water stability solubility ascribed to their...

ABSTRACT 3-fucosyllactose (3-FL) and 2'-fucosyllactose (2'-FL), are two important fucosylated oligosaccharides in human milk. Extensive studies on 2'-FL enabled its official approval for use infant formula. However, development of 3-FL has been somewhat sluggish due to low content milk poor yield enlarged production. Here, an α-1,3-fucosyltransferase mutant was introduced into engineered Escherichia coli (E. coli) capable producing GDP-L-fucose, leading a promising titer 5.0-L bioreactor. To...

Abstract We report the discovery of a possible accretion stream toward Milky Way–type galaxy M106 based on very deep H i imaging data with Five-hundred-meter Aperture Spherical radio Telescope (FAST). The extends for about 130 kpc in projection length and it is similar to Magellanic many respects. provide unambiguous evidence morphology, kinematics local star formation activity show that gas being accreted onto disk M106. Such long continuous flow provides unique opportunity probe...

We attempt to visually classify the morphologies of 18190 molecular clouds, which are identified in $^{12}$CO(1-0) spectral line data over $\sim$ 450 deg$^{2}$ second Galactic quadrant from Milky Way Imaging Scroll Painting project (MWISP). Using velocity-integrated intensity maps emission, clouds first divided into unresolved and resolved ones. The further classified as non-filaments or filaments. Among 25 $\%$ unresolved, 64$\%$ non-filaments, 11$\%$ In terms integrated flux spectra whole...

(1) Background: Ellagic acid (EA) acts as a product of gut microbiota transformation to prevent insulin resistance, which is limited by high-fat diet (HFD)-induced dysbiosis. The aim this study was investigate the synergistic effects and mechanisms supplementation with probiotic Weizmannia coagulans (W. coagulans) on prevention resistance EA; (2) Methods: C57BL/6J mice were divided into five groups (n = 10/group): low-fat-diet group, high-fat-diet EA intervention + W. BC77 BC2000 group; (3)...

ABSTRACT We report the discovery of a 100 kpc H i tail in merging galaxy pair NGC 4490/85 detected by Five-Hundred-meter Aperture Spherical radio Telescope (FAST). The tidal tails extended both south and north directions, they are much longer than that reported previously based on VLA interferometric maps. is surrounded large gas envelope, starburst low-metallicity dwarf MAPS 1231+42 found to be connected with indicating interaction trigged intense star formation it. Based fact metallicity...

Abstract We present a catalog of 32,162 12 CO molecular clouds that covers the northern outer Galactic plane ( l = [15°, 165°] and [195°, 230°], b [−5.°25, +5.°25]). The was produced using DBSCAN algorithm applied to Milky Way Imaging Scroll Painting project data. systematically analyze both integrated properties scaling relationships these across different Galactocentric radii (8 &lt; R GC 26 kpc). An interesting finding is each cloud property’s mean, median, maximum values generally...

The objective of this study was to investigate the stability and degradation kinetics N-acetylneuraminic acid (Neu5Ac). pH solution strongly influenced Neu5Ac, which more stable at neutral low temperatures. Here, we provide detailed information on Neu5Ac different values (1.0, 2.0, 11.0 12.0) temperatures (60, 70, 80 90 °C). under acidic conditions (pH 1.0–2.0) is highly pertinent for hydrolysis polysialic acid. alkaline deacetylation were also studied. 3.0–10.0, even high temperature, but...