ABSTRACT The antifungal substances SH-1 and SH-2 were isolated from Streptomyces humidus strain S5-55 cultures by various purification procedures identified as phenylacetic acid sodium phenylacetate, respectively, based on the nuclear magnetic resonance, electron ionization mass spectral, inductively coupled plasma spectral data. completely inhibited growth of Pythium ultimum, Phytophthora capsici, Rhizoctonia solani, Saccharomyces cerevisiae , Pseudomonas syringae pv. at concentrations 10...

The glycolipid antibiotic rhamnolipid B isolated from Pseudomonas aeruginosa strain B5 was evaluated for in vitro antifungal activity and vivo control against phytophthora blight anthracnose under glasshouse conditions. Rhamnolipid showed Cercospora kikuchii, Cladosporium cucumerinum, Colletotrichum orbiculare, Cylindrocarpon destructans, Magnaporthe grisea Phytophthora capsici. Microscopic observation revealed that the high level of (10 µg ml −1) P capsici mainly due to a lytic effect on...

Abstract As environmental and commercial requirements for new fungicides are increasingly demanding, antifungal compounds of microbial origin attract tremendous interest as a starting point in the development environmentally sound agricultural fungicides. seen fenpiclonil, fludioxonil synthetic derivatives strobilurins such azoxystrobin krexosim‐methyl, this approach has proven to be promising effective strategy developing result, metabolites face revival lead compounds. Recently, numerous...

Abstract The Xanthomonas campestris pv vesicatoria (Xcv) effector AvrBsT induces a hypersensitive cell death in pepper (Capsicum annuum). However, the molecular mechanisms underlying AvrBsT-triggered are not fully understood. Here, we identified arginine decarboxylase (CaADC1) as an AvrBsT-interacting protein, which is early and strongly induced incompatible pepper-Xcv interactions. Bimolecular fluorescence complementation coimmunoprecipitation assays showed that CaADC1-AvrBsT complex was...

Burkholderia glumae is the primary causal agent of bacterial panicle blight rice. In this study, 11 naturally avirulent and nine virulent strains B. native to southern United States were characterized in terms virulence rice onion, toxofalvin production, antifungal activity, pigmentation genomic structure. Virulence on panicles was highly correlated onion bulb scales, suggesting that can be a convenient alternative host system efficiently determine strains. Production toxoflavin, phytotoxin...

The pikromycin biosynthetic gene cluster contains the pikAV encoding a type II thioesterase (TEII). TEII is not responsible for polyketide termination and cyclization, its role has been unclear. During biosynthesis, extender units such as methylmalonyl acyl carrier protein (ACP) may prematurely decarboxylate to generate corresponding acyl-ACP, which cannot be used substrate in condensing reaction by ketosynthase domain, rendering synthase module inactive. It proposed that serve an “editing”...

Hahella chejuensis KCTC 2396 produces red pigments, showing antibacterial and algicidal activities. The main red-coloured metabolite of the pigments was identified as antibiotic prodigiosin. With expectation that are a mixture series close relatives, aim present study is to detect new prodigiosin analogues analyse biosynthetic pattern for prodiginines in 2396.Except prodigiosin, other constituents were confirmed well-known dipyrrolyldipyrromethene norprodigiosin, undecylprodiginine....

We previously selected rhizobacterial strains CCR04, CCR80, GSE09, ISE13, and ISE14, which were antagonistic to Phytophthora blight of pepper. In this study, we investigated the effects root treatment rhizobacteria on anthracnose occurrence, ripening, yield pepper fruit in plastic house field 2008 2009. also examined volatiles produced by ripening mycelial growth spore development Colletotrichum acutatum capsici laboratory, identifying volatile compounds gas chromatography-mass spectrometry...

In our previous studies, we observed the biological control effect of lactic acid bacteria strains (LABs) KLF01, KLC02 and KPD03 against different plant pathogenic in vitro Ralstonia solanacearum, KLF01 Pectobacterium carotovorum under greenhouse field experiments, respectively. this study, efficacy these bacterial spot pathogen (Xanthomonas campestris pv. vesicatoria) their growth-promoting activities pepper (Capsicum annuum L. var. annuum), conditions. LABs significantly (P < 0.05) reduced...

Antimicrobial cyclic peptides derived from microbes bind stably with target sites, have a tolerance to hydrolysis by proteases, and favorable degradability under field conditions, which make them an attractive proposition for use as agricultural fungicides. are classified according the types of bonds within ring structure; homodetic, heterodetic, complex peptides, in turn reflect diverse physicochemical features. Most antimicrobial affect integrity cell envelope. This is achieved through...

This study examined the role of periplasmic oxidative defense proteins, copper, zinc superoxide dismutase (SodC), and thiol peroxidase (Tpx), from Shiga toxin-producing Escherichia coli O157:H7 (STEC) in formation biofilms. Proteomic analyses have shown significantly higher expression levels both antioxidant systems (SodC Tpx) STEC cells grown under biofilm conditions than planktonic conditions. An analysis their growth phase-dependent gene indicated that a high level sodC occurred during...

Phoslactomycins (PLMs), potent and selective inhibitors of serine threonine phosphatases, are interest for their antitumor antiviral activity. Multiple analogs low titers in the fermentation process have hampered development this class natural products. The entire 75-kb PLM biosynthetic gene cluster Streptomyces sp. HK-803 was cloned, sequenced, analyzed. loading domain seven extension modules polyketide synthase generate an unusual linear unsaturated chain containing both E- Z-double bonds...

Nine isolates of Phytophthora capsici obtained from pumpkin and pepper in diverse geographic areas, including Korea, France, Italy, the United States, were evaluated for their ability to cause disease on nine Korean Japanese cultivars under controlled environmental conditions. No hypersensitive type resistance was observed any inoculated with P. capsici. Disease incidence ranged low high, indicating varying levels partial (quantitative) resistance. In addition, a significant cultivar-isolate...

Most cancer cells show resistance to ionizing radiation (IR)-induced cell death. Recently, Ki-Ras was reported be responsible for the increased radioresistance. We report here that inhibition of IR-induced activaton nuclear transcription factor kappa B (NF-κB) but not either Akt or MAPK kinase (MEK), radiosensitization transformed human prostate epithelial 267B1/K-ras cells. Proteosome inhibitor-1 (Pro1) reduced NF-κB activation, and this accompanied by levels cytoplasmic IκBα p65/RelA....

Inhibition of protein tyrosine phosphatase 1B (PTP1B) has been proposed as a therapy for treatment type 2 diabetes and obesity. Bioassay-guided fractionation the MeOH extract leaves stems Symplocos paniculata (Thunb.) Miq. (Symplocaceae), using an in vitro PTP1B inhibitory assay, resulted isolation three ursane-type triterpenes, ursolic acid (1), corosolic (2) 2α,3α,19α,23-tetrahydroxyurs-12-en-28-oic (3). Compounds 1 - 3 inhibited with IC50 values 3.8 ± 0.5, 7.2 0.8 42.1 1.5 μM,...