A5016274238- Asthma and respiratory diseases
- Immune Response and Inflammation
- Inflammatory mediators and NSAID effects
- Eicosanoids and Hypertension Pharmacology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neurobiology and Insect Physiology Research
- Single-cell and spatial transcriptomics
- Retinal Development and Disorders
- Cell Image Analysis Techniques
- Immune Cell Function and Interaction
- Adenosine and Purinergic Signaling
- Estrogen and related hormone effects
- Mast cells and histamine
- Ion Transport and Channel Regulation
- Antifungal resistance and susceptibility
- Organic Chemistry Cycloaddition Reactions
- S100 Proteins and Annexins
- Bioinformatics and Genomic Networks
- Spectroscopy and Chemometric Analyses
- Hormonal Regulation and Hypertension
- Genomics and Chromatin Dynamics
- Nitric Oxide and Endothelin Effects
- NF-κB Signaling Pathways
- Metabolomics and Mass Spectrometry Studies
- Pregnancy and preeclampsia studies
Cégep de Sherbrooke
2025
Bristol-Myers Squibb (United States)
2024
Janelia Research Campus
2012-2020
Howard Hughes Medical Institute
2012-2020
Princeton University
2016-2019
Institute for Integrative and Experimental Genomics
2019
Broad Institute
2014
Université Européenne de Bretagne
2013
Helix (United States)
2012
Université Laval
1999-2011
The anatomy of many neural circuits is being characterized with increasing resolution, but their molecular properties remain mostly unknown. Here, we characterize gene expression patterns in distinct cell types the Drosophila visual system using genetic lines to access individual types, TAPIN-seq method measure transcriptomes, and a probabilistic interpret these measurements. We used tools build resource high-resolution transcriptomes for 100 driver covering 67 available at...
Rod and cone photoreceptors are highly similar in many respects but they have important functional molecular differences. Here, we investigate genome-wide patterns of DNA methylation chromatin accessibility mouse rods cones correlate differences these features with gene expression, histone marks, transcription factor binding, sequence motifs. Loss NR2E3 shifts their epigenomes to a more cone-like state. The data further reveal wide between retinal brain neurons. Surprisingly, also find...
Many tools are available to analyse genomes but often challenging use in a cell type–specific context. We have developed method similar the isolation of nuclei tagged specific type (INTACT) technique [Deal,R.B. and Henikoff,S. (2010) A simple for gene expression chromatin profiling individual types within tissue. Dev. Cell , 18 1030–1040; Steiner,F.A., Talbert,P.B., Kasinathan,S., Deal,R.B. (2012) Cell-type-specific purification from whole animals genome-wide profiling. Genome Res .,...
is a database of gene signatures that have been extracted and manually curated from the published literature.It provides standardized resource prognostic, diagnostic other cancer related disease to community so they can compare predictive power or use these in set enrichment analysis.Since GeneSigDB release 1.0, we expanded 575 3515 signatures, which were collected transcribed 1604 articles largely focused on expression cancer, stem cells, immune development lung disease.We made substantial...
Understanding the principles governing neuronal diversity is a fundamental goal for neuroscience. Here, we provide an anatomical and transcriptomic database of nearly 200 genetically identified cell populations. By separately analyzing robustness pattern expression differences across these populations, identify two gene classes contributing distinctly to diversity. Short homeobox transcription factors distinguish populations combinatorially, exhibit extremely low transcriptional noise,...
An exo-(1->4)-[beta]-D-galactanase was isolated from ripe tomato fruit (Lycopersicon esculentum Mill. cv Ailsa Craig and Better Boy) using anion-exchange, gel filtration, cation-exchange chromatography. Sodium dodecyl sulfate-polyacrylamide electrophoresis of the most active fraction revealed a predominant protein band at 75 kD several minor bands. A 30-amino acid N-terminal sequence this 75-kD showed high degree homology with other recently identified [beta]-galactosidase/galactanase...
Abstract We have described in det ail the secretory activity of leukotriene B4 toward rabbit neutrophils. Leukotriene rapidly and vigorously degranulates This is stereospecific, cytochalasin B-dependent, enhanced by extracellular calcium. Pretreatment with deactivates neutrophils, i.e., cells so treated do not respond to stimulation an additional bolus B4. In addition, sharply dependent on simultaneous presence B. Rabbit neutrophils therefore exhibit previously desensitization effect these...
Species of the Drosophila melanogaster species subgroup, including D. simulans, mauritiana, yakuba, and santomea, have long served as model systems for studying evolution. However, studies in these been limited by a paucity genetic transgenic reagents. Here, we describe collection strains generated to facilitate within between species. We many each containing mapped piggyBac transposons an enhanced yellow fluorescent protein (EYFP) gene expressed eyes ϕC31 attP site-specific integration...
The 17th Workshop on Recent Issues in Bioanalysis (17th WRIB) took place Orlando, FL, USA June 19–23, 2023. Over 1000 professionals representing pharma/biotech companies, CROs, and multiple regulatory agencies convened to actively discuss the most current topics of interest bioanalysis. WRIB included 3 Main Workshops 7 Specialized that together spanned 1 week allow an exhaustive thorough coverage all major issues bioanalysis biomarkers, immunogenicity, gene therapy, cell therapy vaccines....
5-Lipoxygenase (5-LO) catalyzes the transformation of arachidonic acid to leukotrienes (LT). In stimulated human PMN, activation 5-LO involves calcium, p38 MAP kinase (p38) phosphorylation, and translocation from cytosol nuclear membranes containing activating protein (FLAP). this study, cAMP-elevating agents such as isoproterenol, prostaglandin E<sub>2</sub>, CGS-21680 (an adenosine A<sub>2a</sub> receptor agonist), type IV phosphodiesterase inhibitor RO 20-1724, adenylate cyclase activator...
Arachidonic acids (AA) and linoleic (LAs) are metabolized, in several tissues, to hydroxylated metabolites that important mediators of many physiological pathophysiological processes. The conjugation leukotriene B4 (LTB4), 5-hydroxyeicosatetraenoic acid (HETE), 12-HETE, 15-HETE, 13-hydroxyoctadecadienoic (HODE) by the human UDP-glucuronosyltransferase (UGT) enzymes was investigated. All substrates tested were efficiently conjugated liver microsomes polar derivatives containing glucuronyl...
Recent studies have shown that alveolar macrophages (AM) are able to release leukotrienes (LTs). Since cigarette smoking inhibits the cyclooxygenase pathway of arachidonic acid metabolism in AM, we evaluated LT production by AM from smokers and nonsmokers. were obtained 35 volunteers, 16 nonsmokers, 19 smokers. The cells incubated under various conditions including stimulation with 30 microM acid, 2 ionophore A23187, or both. Each experiment was performed parallel using a smoker nonsmoker....
ABSTRACT Toll‐like receptors (TLR) recognize pathogen‐associated molecular patterns and play important roles in the innate immune system. While single‐stranded viral RNA is natural ligand of TLR7/TLR8, imidazoquinoline resiquimod (R‐848) recognized as a potent synthetic agonist TLR7/TLR8. We investigated effects TLR7/8 activation on lipid mediator production polymorphonuclear leukocytes exposed to R‐848. Although R‐848 had minimal by itself, it strongly enhanced leukotriene B 4 formation...
The ability of the major neutrophil-derived lipoxygenase metabolites arachidonic acid to increase rate 45Ca influx in rabbit neutrophils was examined. results obtained demonstrate that (5S),(12R)-dihydroxy-6,8,11,14-(cis,trans,trans,cis)-eicosatetraenoic (leukotriene B4) is most active metabolites. activity leukotriene B4 highly stereospecific its three nonenzymatically derived isomers are essentially inactive. omega-hydroxylation a compound nearly as far stimulate calcium and neutrophil...
There is increasing evidence of a role for Toll-like receptors (TLRs) in inflammatory arthritis. The extra domain A (ED-A)-containing isoform fibronectin generated under pathologic conditions such as rheumatoid arthritis, and ED-A has been identified an endogenous TLR-4 ligand. Leukotriene B4 (LTB4) polymorphonuclear neutrophils (PMNs) play critical murine models aim this study was therefore to investigate the putative effects on leukotriene biosynthesis PMN migration through TLR...
RNA-seq has become the standard tool for collecting genome-wide expression data in diverse fields, from quantitative genetics and medical genomics to ecology developmental biology. However, library preparation is still prohibitive many laboratories. Recently, field of single-cell transcriptomics reduced costs increased throughput by adopting early barcoding pooling individual samples -producing a single final containing all samples. In contrast, protocols where each sample processed...
Adenosine (Ado) has been shown to suppress several functional responses of human polymorphonuclear leukocytes (PMNs). The current study investigated whether endogenous Ado regulates the biosynthesis leukotriene (LT)B4 in ligand-stimulated PMNs. Measurements PMN resuspended Hanks' buffered salt solution (HBSS) or plasma showed a cell concentration– and time–dependent accumulation nucleoside. removal with either deaminase blockade its action by A2a receptor antagonist, 8-(3-chlorostyryl)...
We report here that the apparent inability of isolated human polymorphonuclear leukocytes (PMNs) to efficiently transform arachidonic acid (AA) is consequence A<sub>2a</sub> receptor engagement by endogenous adenosine accumulating in incubation media. Indeed, when eliminated from PMN suspensions addition deaminase, or cells are incubated with antagonists, important quantities (40–80 pmol/10<sup>6</sup> cells) 5-lipoxygenase products synthesized 1 5 μM exogenous AA. The selective agonist...