A5063947325- Inhalation and Respiratory Drug Delivery
- RNA Interference and Gene Delivery
- Neonatal Respiratory Health Research
- Drug Solubulity and Delivery Systems
- Microencapsulation and Drying Processes
- Asthma and respiratory diseases
- Advanced Drug Delivery Systems
- Advanced biosensing and bioanalysis techniques
- Advancements in Transdermal Drug Delivery
- Natural product bioactivities and synthesis
- Cancer Research and Treatments
- Helicobacter pylori-related gastroenterology studies
- Infection Control and Ventilation
- Antimicrobial agents and applications
- Tannin, Tannase and Anticancer Activities
- Combustion and Detonation Processes
- Cancer, Hypoxia, and Metabolism
- Renal and related cancers
- Hepatocellular Carcinoma Treatment and Prognosis
- Electron and X-Ray Spectroscopy Techniques
- Protein purification and stability
- Refrigeration and Air Conditioning Technologies
- Occupational and environmental lung diseases
- Industrial Vision Systems and Defect Detection
- Retinoids in leukemia and cellular processes
Meijo University
2015-2025
Hitachi High-Tech (Japan)
2023-2024
Hitachi (Japan)
2023-2024
The University of Sydney
2016-2017
Kyoto University
2008-2009
Okayama University
1990-2006
University of Fukui
1997-2006
Showa University
1990
ABSTRACT Colonization of the stomach mucosa by Helicobacter pylori is a major cause acute and chronic gastric pathologies in humans. Several H. virulence genes that may play role its pathogenicity have been identified. The most important determinants are vacA cagA cag island ( PAI) genes. In present study, to consider association molecular genetics between PAI regarding clinical outcome, we selected strains with various genotypes Japan sequenced full-length , cagE Sequencing revealed...
In this study, a novel dry small interfering RNA (siRNA) powder for inhalation, containing chitosan and mannitol, was prepared using the supercritical carbon dioxide (CO2) technique. Although siRNA/chitosan difficult to disperse because of long needle-like structure, it could be reduced fragments 10-20 µm by manual grinding, which allowed administration into mice. Electrophoresis revealed that CO2 technique grinding didn't greatly affect integrity siRNA. Furthermore, siRNA more stable in...
In this study, two types of biodegradable polycation (PAsp(DET) homopolymer and PEG-PAsp(DET) copolymer) were applied as vectors for inhalable dry gene powders prepared by spray freeze drying (SFD). The had spherical porous structures with a 5~10-μm diameter, the integrity plasmid DNA could be maintained during powder production. Furthermore, it was clarified that PEG-PAsp(DET)-based more sufficiently maintain both physicochemical properties in vitro transfection efficiencies polyplexes...
Forty-five ellagitannins and related compounds were intraperitoneally injected into mice once, 4 d before intraperitoneal inoculation of S-180 cells, their antitumor activities evaluated. When an antitumor-active tannin was defined as one producing a 70% increase or more in the mean life span regressor out six mice, twenty-one active. Among monomeric ellagitannins, tellimagrandin II most Most oligomeric consisting tellimagrandins I monomer unit, had significant activity. Macrocyclic all...
Gene therapy is a breakthrough treatment strategy against several intractable and lethal diseases that previously lacked established treatments. Viral nonviral vectors have been studied to realize higher gene transfection efficiencies suppress the degradation of by nucleolytic enzymes in vivo. However, it often case addition vector results adverse effects. In this study, we identified formulations dry naked plasmid DNA (pDNA) powders with no showing significantly expression than pDNA...
Abstract Extracellular histones promote platelet aggregation and thrombosis; this is followed by induction of coagulation disorder, which results in exhaustion factors. Complement component 5 (C5) known to be associated with system activation. To date, the pathological mechanism underlying liver injury has remained unclear. Here, we investigated whether C5 promotes histone-induced lethal thrombosis. C5-sufficient C5-deficient mice received single tail vein injections purified, unfractionated...
Spray-freeze-drying (SFD) is a unique powderization technique to produce highly porous dry powders with low density. The characteristic morphology can markedly contribute the superior inhalation performances of SFD powders. Due increased specific surface area powders, however, moisture adsorption may readily occur, subsequently leading losses their potentials. In this study, hydrophobic amino acids were newly applied as pharmaceutical excipients obtain both favorable performance and...
Gene therapy using vectors has attracted attention in recent years for the treatment of cancers caused by gene mutations. Besides, new treatments are imperative lung cancer, including non-small cell cancer (NSCLC) and malignant pleural mesothelioma (MPM), due to its high mortality. We developed a minimally invasive orally inhalable tumor suppressor drug (SFD-p16 SFD-p53) with non-viral combining genes an inhalant powder that can deliver active ingredients directly lung. used NSCLC (A549...
The aim of this study was to reveal the relationship between human inspiratory flow patterns and concomitant drops in pressure different inhalation devices, influence devices on performance. As a model formulation for inhalers, physically mixed dry powder composed salbutamol sulfate coarse lactose monohydrate selected. at 28.3 L/min three Single-type, Dual-type, Reverse-type, 1.0, 5.1, 8.7 kPa, respectively. Measurements revealed that although least resistant device (Single) had large inter-...