A5100435290- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- Glycosylation and Glycoproteins Research
- Advanced Mathematical Physics Problems
- Nonlinear Photonic Systems
- Nonlinear Waves and Solitons
- Cancer-related gene regulation
- Aldose Reductase and Taurine
- HER2/EGFR in Cancer Research
- Photosynthetic Processes and Mechanisms
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Protein purification and stability
- CAR-T cell therapy research
- Histone Deacetylase Inhibitors Research
- Essential Oils and Antimicrobial Activity
- Genomics and Chromatin Dynamics
- Spectroscopy Techniques in Biomedical and Chemical Research
- Stability and Controllability of Differential Equations
- Spectroscopy and Quantum Chemical Studies
- Cancer Mechanisms and Therapy
- Hemoglobin structure and function
- Electrochemical sensors and biosensors
- RNA Research and Splicing
- Plant-Microbe Interactions and Immunity
Shanghai Pudong New Area Gongli Hospital
2024
Second Military Medical University
2024
Guangdong Pharmaceutical University
2015-2023
Xuzhou Medical College
2022
Lianyungang Oriental Hospital
2022
Shanghai Ninth People's Hospital
2022
Shanghai Jiao Tong University
2022
Lanzhou University
2022
Sun Yat-sen University
2009-2021
Zhejiang A & F University
2021
Expansions of myeloid-derived suppressor cells (MDSCs) have been identified in human solid tumors, including colorectal cancer (CRC). However, the nature these tumor-associated MDSCs and their interactions with tumor CRC are still poorly understood.The percentages phenotype peripheral blood tumorous paraneoplastic tissues from patients, as well clinical relevance MDSCs, were assessed. Age-matched healthy donors included controls. The interaction between T or was investigated a coculture...
To solve the problems of low bioavailability and unstable properties Cinnamomum cassia Essential oil (CCEO), encapsulation technology was introduced as an effective means to improve its shortcomings. In this study, nano-emulsion (CCEO-NE) successfully synthesized by oil-in-water method characterized standard analytical methods, including dynamic light scattering (DLS), Scanning electron microscopy (SEM), Transmission (TEM). The results show that CCEO is spherical, smooth in surface, uniform...
The antineoplastic target aldo-keto reductase family member 1B10 (AKR1B10) and the critical polyol pathway enzyme aldose (AKR1B1) share high structural similarity. Crystal structures reported here reveal a surprising Trp112 native conformation stabilized by specific Gln114-centered hydrogen bond network in AKR1B10 holoenzyme, suggest that AKR1B1 inhibitors could retain their binding affinities toward inducing flip to result an "AKR1B1-like" active site AKR1B10, while selective can take...
Background: N6-methyladenosine (m6A) is one of the most prevalent mRNA modifications in mammals, and it regulates fate modified RNA transcripts.In current study, we aimed to elucidate role YTH m6A RNA-binding protein 1 (YTHDF1), a "reader" modification, prostate cancer tumorigenesis.Methods: We employed multi-omics approach detect direct target YTHDF1 upon manipulation expression cells.Expression was also evaluated human tumors either adjacent or paired normal tissues.Additionally, vivo...
Abstract Background The fungus Marssonina brunnea is a causal pathogen of leaf spot that devastates poplar plantations by defoliating susceptible trees before normal fall drop. Results We sequence the genome M. with size 52 Mb assembled into 89 scaffolds, representing first sequenced Dermateaceae genome. By inoculating this onto hybrid clone, we investigate how interacts and co-evolves its host to colonize leaves. While handful virulence genes in , mostly from LysM family, are detected...
Muc1 is one of the most studied tumor antigens. However, antibodies or antibody-toxin conjugates against have not shown significant efficacy for tumors with overexpression. In this study, we employed bispecific antibody approach to target positive cells. A novel antibody, Muc1-Bi-1, was constructed by linking single domain antibodies, anti-Muc1-VHH and anti-CD16-VHH. Muc1-Bi-2, humanized form also grafting. Both Muc1-Bi can be efficiently expressed purified from bacteria. vitro, recruit...
N6-Methyladenosine (m6A) imbalance is an important factor in the occurrence and development of prostate cancer (PCa). Many m6A regulators have been found to be significantly dysregulated PCa. ELAVL1 binding protein that can promote tumors m6A-dependent manner. In this study, we most were PCa, some associated with progression-free interval. Mutations copy number variations these alter their expression. However, mutations not Nevertheless, upregulation was closely related PCa proliferation....
The environment of the CuI ion in distal ligand group decides fate reduction O2 by two analogues 1 and 2 heme a3 CuB center cytochrome c oxidase. fourfold coordination N favors CuII oxidation state leads to a 4 e−–4 H+ formation H2O under physiological conditions, while with e−–2 occurs form cytotoxic H2O2.
Bispecific antibodies have emerged as powerful therapeutic agents given their high specificity and ability to induce a potent immune response. Various bispecific antibody formats been designed studied regarding applications in cancer therapy, though associated with issues of short half-life or manufacturing difficulties. Herein, novel antibody, SS-Fc, was constructed by pairing 2 single-domain antibodies, anti-CD16 anti-CEA, which were fused CH3 "knobs into holes" mutations individually....
Bispecific antibodies that engage immune cells to kill cancer are actively pursued in immunotherapy. Different types of bispecific antibodies, including single-chain fragments, Fab nanobodies, and immunoglobulin Gs (IgGs), have been studied. However, the low molecular weight with or fragments generally leads their rapid clearance vivo, which limits therapeutic potential these antibodies.In this study, we used a site-specific PEGylation strategy modify single-domain antibody-linked (S-Fab),...
Indoleamine 2,3-dioxygenase (IDO), an immune checkpoint, is a promising target for cancer immunotherapy.
Her2, which is frequently overexpressed in breast cancer, one of the most studied tumor-associated antigens for cancer therapy. Anti-HER2 monoclonal antibody, trastuzumab, has achieved significant clinical benefits metastatic cancer. In this study, we describe a novel bispecific antibody Her2-S-Fab targeting Her2 by linking single domain anti-CD16 VHH to trastuzumab Fab. The can be efficiently expressed and purified from Escherichia coli, drive potent cell killing HER2-overexpressing cells....
CD16a (FcγRIIIa) mediates the antibody dependent cellular cytotoxicity (ADCC) and is important for anti-tumor activities of many therapeutic antibodies. Bispecific targeting natural killer (NK) cells has been studied cancer therapy. In this work, anti-CD16a single-domain antibodies were identified from hCD16a immunized camel. are then constructed by fusing these single domain with an anti-CEA antibody. These bispecific can recruite NK to kill CEA-positive tumor cells, inhibit growth in vivo,...
Bispecific antibodies that engage immune cells to kill cancer have been actively studied in immunotherapy. In this study, we present a novel bispecific format, S-Fab, fabricated by linking single-domain anti-carcinoembryonic antigen VHH conventional anti-CD3 Fab. contrast most antibodies, the S-Fab antibody can be efficiently expressed and purified from bacteria. The is stable serum able recruit T drive potent cell killing. xenograft models, suppresses tumor growth presence of human cells....
Measurement of optical properties is critical for understanding light-tissue interaction, properly interpreting measurement data, and gaining better knowledge tissue physicochemical properties. However, conventional measuring techniques are limited in point measurement, which partly hinders the applications on characterizing spatial distribution inhomogeneity biological tissues. Spatial-frequency domain imaging (SFDI), as an emerging non-contact, depth-varying wide-field technique, capable a...