A5108679480- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Salmonella and Campylobacter epidemiology
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- Antibiotics Pharmacokinetics and Efficacy
- Biosensors and Analytical Detection
- COVID-19 diagnosis using AI
- SARS-CoV-2 and COVID-19 Research
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Peptides and Activities
- Antibiotic Use and Resistance
- Poxvirus research and outbreaks
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial Infections and Vaccines
- Reproductive tract infections research
- Bacillus and Francisella bacterial research
- Urinary Tract Infections Management
- Tuberculosis Research and Epidemiology
- Gastrointestinal motility and disorders
- Syphilis Diagnosis and Treatment
- Pneumonia and Respiratory Infections
- Drug Transport and Resistance Mechanisms
- Nephrotoxicity and Medicinal Plants
Mahidol University
2020-2025
Chulalongkorn University
2017-2021
King Chulalongkorn Memorial Hospital
2019-2020
Abstract Tigecycline has been regarded as one of the most important last-resort antibiotics for treatment infections caused by extensively drug-resistant (XDR) bacteria, particularly carbapenem- and colistin-resistant Klebsiella pneumoniae (C-C-RKP). However, reports on tigecycline resistance have growing. Overall, ~ 4000 K. clinical isolates were collected over a five-year period (2017–2021), in which 240 C-C-RKP investigated. Most these (91.7%) resistant to tigecycline. Notably, high-risk...
The global prevalence of colistin-resistant Klebsiella pneumoniae (ColRkp) facilitated by chromosomal and plasmid-mediated Ara4N or PEtN-remodeled LPS alterations has steadily increased with colistin usage for treating carbapenem-resistant K. (CRkp). Our study demonstrated the rising trend ColRkp showing extensively pandrug-resistant characteristics among CRkp, a 28.5%, which was mediated mgrB, pmrB, phoQ mutations (91.5%), mcr-1.1, mcr-8.1, mcr-8.2 alone in conjunction R256G PmrB (8.5%)....
Pseudomonas aeruginosa infections in the lungs affect millions of children and adults worldwide. To our knowledge, no clinically validated prognostic biomarkers for chronic pulmonary P. exist. Therefore, this study aims to identify potential markers biofilm lung infections. Here, we screened expression 11 regulatory genes (tesG, algD, lasR, lasA, lasB, pelB, phzF, rhlA, rsmY, rsmZ, sagS) associations between clinical status infection. RNA was extracted from 210 sputum samples patients (n =...
Overcoming colistin-resistant Acinetobacter baumannii (CoR-AB) has become a major concern due to the lack of effective antibiotics. This study aimed explore prevalence CoR-AB clinical isolates in Thailand, their mechanisms resistance, and test efficacy colistin plus sulbactam against isolates. The resistance rate among carbapenem-resistant A. was 15.14%. mcr gene or its variants were not detected by PCR screening. lipid A mass spectra showed additional [M-H]- ion peak at m/z = 2034 that...
The monkeypox virus is excreted in the feces of infected individuals. Therefore, there an interest using viral load detection wastewater for sentinel early surveillance at a community level and as complementary approach to syndromic surveillance. We collected from 63 sewered non-sewered locations Bangkok city center between May August 2022. Monkeypox DNA copy numbers were quantified real-time polymerase chain reaction (PCR) confirmed positive by Sanger sequencing. was first detected second...
Discover the shifting landscape of SARS-CoV-2 variants from October to December 2023, with JN.1 dominating South and Southeast Asia wastewater samples, increasing <10% >90%. Experience dynamic evolution viral strains in this period.
SARS-CoV-2 RNA is excreted in feces of most patients, therefore viral load wastewater can be used as a surveillance tool to develop an early warning system help and manage future pandemics.
Abstract Development of an effective therapy to overcome colistin resistance in Klebsiella pneumoniae , a common pathogen causing catheter-related biofilm infections vascular catheters, has become serious therapeutic challenge that must be addressed urgently. Although and EDTA have successful roles for eradicating biofilms, no vitro vivo studies investigated their efficacy colistin-resistant K. . In this study, was significantly reversed both planktonic mature biofilms by combination (0.25–1...
Introduction Infections caused by drug-resistant Klebsiella pneumoniae are now a serious problem for public health, associated with high morbidity and mortality due to limited treatment options. Therefore, new antibacterial agents or combination of as the first line urgently needed. K11 is novel antimicrobial peptide (AMP) that has demonstrated in vitro activity against several types bacteria. Additionally, previously shown no hemolytic activity. Herein, activity, synergistic action...
High population density and tourism in Southeast Asia increase the risk of mpox due to frequent interpersonal contacts. Our wastewater surveillance six Asian countries revealed positive signals for Monkeypox virus (MPXV) DNA, indicating local transmission. This alerts clinicians helps allocate resources like testing, vaccines therapeutics resource-limited countries.
Developing an effective therapy to overcome carbapenemase-positive Klebsiella pneumoniae (CPKp) is important therapeutic challenge that must be addressed urgently. Here, we explored a Ca-EDTA combination with aztreonam or ceftazidime-avibactam in vitro and vivo against diverse CPKp clinical isolates. The synergy testing of this study demonstrated novel aztreonam-Ca-EDTA ceftazidime-avibactam-Ca-EDTA was significantly eliminating planktonic mature biofilms vitro, as well eradicating...
Abstract Despite strengthened antimicrobial therapy, biofilm infections of Acinetobacter baumannii are associated with poor prognosis and limited therapeutic options. Assessing antibiotics on planktonic bacteria can result in failure against infections. Currently, to treat administered empirically, usually without considering the susceptibility objectively before beginning treatment. For effective therapy resolve it is essential assess efficacy commonly used biofilms. Here, we offer a robust...
Abstract The global rapid emergence of azithromycin/ceftriaxone resistant Neisseria gonorrhoeae threatens current recommend dual therapy for gonorrhea to ensure effective treatment. Here, we identified the first two N. isolates with decreased ceftriaxone susceptibility in Thailand. Among 134 collected from Thai Red Cross Anonymous Clinic, Bangkok, (NG-083 and NG-091) urethral swab male heterosexual patients had reduced (MICs 0.125 mg/L). Both were multidrug strong biofilm producers tolerance...
The study explored SARS-CoV-2 viral concentrations in the dust as a surveillance tool Lockdown reduced concentrations, curbing spread Viral levels dropped public areas but transmission persisted Our results provide another non-invasive avenue for monitoring COVID-19 outbreaks
Treatment of infections by Pseudomonas aeruginosa forming biofilms after antimicrobial testing on planktonic bacteria can result in substantial failure. Therefore, we offer a robust and simple experimental platform to test the impact antimicrobials biofilms. Antibiotic response patterns varied uniquely within biofilm formation capacity minimal eradication concentrations (MBECs) has significantly better discriminatory power than minimum inhibitory (MICs) differentiate overall efficiency...
Given the rise of multidrug-resistant (MDR) Pseudomonas aeruginosa infections, alternative treatments are needed. Anti-pseudomonal phage therapy shows promise, but its clinical application is limited due to development resistance and a lack biofilm penetration. Recently, adjuvants like CaEDTA have shown ability enhance effectiveness combined antimicrobial agents. Here, we tested phage-adjuvant combination demonstrated intranasally inhaled (KKP10) + in addition ceftazidime/avibactam (CZA) for...
The increasing occurrence of hospital-associated infections, particularly bacteremia, caused by extensively drug-resistant (XDR) carbapenemase-producing colistin-resistant Klebsiella pneumoniae highlights a critical requirement to discover new therapeutic alternatives. Bacteriophages having host-specific bacteriolytic effects are promising alternatives for combating these pathogens. Among 12 phages isolated from public wastewater in Thailand, two phages-vB_kpnM_05 (myovirus) and vB_kpnP_08...
Abstract Nontyphoidal Salmonella , an important zoonotic pathogen and a major cause of foodborne illnesses, could be potential reservoir plasmids harbouring mobile colistin resistance gene ( mcr ). This study reported, for the first time, high rate -carrying clinical isolates (3.3%, 24/724) in Thailand, associated with mcr-3 (3.0%, 22/724) S. 4,[5],12:i:-(15.4%, 4/26), S . Typhimurium (8.8%, 5/57), Choleraesuis (5.6%, 13/231). Remarkably, increasing trends extended-spectrum cephalosporin...
Salmonella and Vibrio cholerae are the deadliest foodborne pathogens, requiring effective routine screening for food safety assurance. Hence, a duplex polymerase chain reaction integrated with lateral flow immunoassay (PCR-LFIA) was developed rapid detections of V. to overcome laborious, time-consuming, complicated, costly limitations standard bacterial culture. The PCR-LFIA could simultaneously detect invA lolB targets at lower limits 0.1 1 ng, respectively. Without culture enrichment,...