A5102871288- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Amino Acid Enzymes and Metabolism
- biodegradable polymer synthesis and properties
- Enzyme Structure and Function
- Fungal and yeast genetics research
- Enterobacteriaceae and Cronobacter Research
- Seaweed-derived Bioactive Compounds
- Polysaccharides and Plant Cell Walls
- Obstructive Sleep Apnea Research
- Viral Infectious Diseases and Gene Expression in Insects
- Probiotics and Fermented Foods
- Enzyme Catalysis and Immobilization
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Pharmacy and Medical Practices
- Neuroscience of respiration and sleep
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Plant Pathogenic Bacteria Studies
- Herpesvirus Infections and Treatments
- Kruppel-like factors research
- Recycling and utilization of industrial and municipal waste in materials production
- Insect and Arachnid Ecology and Behavior
Jiangnan University
2018-2024
Zhejiang A & F University
2023-2024
State Key Laboratory of Food Science and Technology
2018-2024
Taiyuan University of Technology
2024
Nankai University
2021-2022
Academia Sinica
2021
Ministère de l'Agriculture et de la Souveraineté alimentaire
2021
Ministry of Agriculture and Agro Based Industry
2021
Huazhong University of Science and Technology
2016-2018
Tongji Hospital
2013-2018
In metabolic engineering, unbalanced microbial carbon distribution has long blocked the further improvement in yield and productivity of high-volume natural metabolites. Current studies mostly focus on regulating desired biosynthetic pathways, whereas few strategies are available to maximize L-threonine efficiently. Here, we present a strategy guarantee supply reduced cofactors actualize maximization by cellular central pathways. A thermal switch system was designed applied divide whole...
Temperature-sensitive mutant 2-20/32 of Mycobacterium smegmatis mc(2)155 was isolated and genetically complemented with a tuberculosis H37Rv DNA fragment that contained single open reading frame. This frame is designated Rv3265c in the M. genome. shows homology to Escherichia coli gene wbbL, which encodes dTDP-Rha:alpha-D-GlcNAc-pyrophosphate polyprenol, alpha-3-L-rhamnosyltransferase. In E. this enzyme involved O-antigen synthesis, but mycobacteria it required for rhamnosyl-containing...
Corynebacterium glutamicum, a microorganism classified as generally recognized safe for use in the industrial production of food raw materials and additives, has encountered challenges achieving widespread adoption popularization microbial cell factories. These obstacles arise from intricate nature manipulating metabolic flux through conventional methods, such gene knockout enzyme overexpression. To address this challenge, we developed CRISPR/dCpf1-based bifunctional regulation system to...
Escherichia coli is an important strain for L-threonine production. Genetic switch a ubiquitous regulatory tool gene expression in prokaryotic cells. To sense and regulate intracellular or extracellular chemicals, bacteria evolve variety of transcription factors. The key enzymes required biosynthesis E. are encoded by the thr operon. operon could coordinate these genes when short supply cell.The thrL leader elements were applied to iclR, arcA, cpxR, gadE, fadR pykF, while...
Abstract Background Betaine, an osmoprotective compatible solute, has been used to improve l -threonine production in engineered Escherichia coli producer. Betaine supplementation upregulates the expression of zwf encoding glucose-6-phosphate dehydrogenase, leading increase NADPH, which is beneficial for production. In E. , betaine can be taken through ProP encoded by proP or ProVWX proVWX. a H + -osmolyte symporter, whereas ABC transporter. and mediate osmotic stress protection transporting...
Abstract Wild‐type Escherichia coli MG1655 usually does not accumulate l ‐threonine. In this study, the effects of 13 genes related to glucose uptake, glycolysis, TCA cycle, l‐ threonine biosynthesis, or their regulation on ‐threonine accumulation in E. were investigated. Sixteen mutant strains constructed by chromosomal deletion overexpression one more rsd, ptsG, ptsH, ptsI, crr, galP, glk, iclR , and gltA ; plasmid pFW01‐ thrA*BC ‐ rhtC harboring key for biosynthesis secretion was...
Gram-negative bacteria are intrinsically resistant to antibiotics due the presence of cell envelope, but mechanisms this resistance still not fully understood. In study, a series mutants that lack one or more major components associated with envelope were constructed from Escherichia coli K-12 W3110. WJW02 can only synthesize Kdo
Poly-3-hydroxybutyrate is an environmentally friendly polymer with many promising applications and can be produced in Escherichia coli cells after overexpressing the heterologous gene cluster phaCAB. In this study, we found that truncating structure of lipopolysaccharide E. effectively enhance poly-3-hydroxybutyrate production. mutant strains WJW00, WJD00, WJJ00 were constructed by deleting rfaD from strain W3110, DH5α, JM109, respectively. Compared to controls W3110/pDXW-8-phaCAB,...
2-Oxobutyrate (2-OBA), as a toxic metabolic intermediate, generally arrests the cell growth of most microorganisms and blocks biosynthesis target metabolites. In this study, we demonstrated that using acetate bypass to replace pyruvate dehydrogenase complex (PDHc) in Escherichia coli could recharge intracellular acetyl-CoA pool alleviate toxicity 2-OBA. Furthermore, based on crystal structure oxidase (PoxB), two candidate residues substrate-binding pocket PoxB were predicted by computational...
Guanosine (GUO) is neuroprotective when administered acutely for the treatment of cerebral ischemia. The aim present study was to investigate whether delayed administration GUO improved long‑term functional recovery following stroke, as well explore potential underlying mechanisms. (8 mg/kg) or a vehicle intraperitoneally 7 consecutive days beginning 24 h prior photothrombosis‑induced stroke in male C57/B6J mice. Behaviour tests were performed at 1, 3, 7, 14 and 28 post‑stroke. Infarct...
BACKGROUND Angiopoietin-like 4 (ANGPTL4) is neuroprotective when administered acutely for the treatment of cerebral ischemia. The aim present study was to evaluate preventive effects ANGPTL4 on formation brain edema and determine whether it promotes recovery neurological function following intracerebral hemorrhage (ICH). MATERIAL AND METHODS Recombinant human (rhANGPTL4; 40 µg/kg) or a vehicle intraperitoneally 5 min prior bacterial collagenase-induced ICH in male C57/B6J mice. Behavioral...
Understanding the detailed influence of OM on cell envelope and cellular metabolism is important for optimizing E. coli factory many other microorganisms. This study revealed applicability remodeling to enhance PHB accumulation as representative inclusion bodies.
The transport of nucleosides and their homeostasis within the cell are essential for growth proliferation. Here, we have identified a novel transcription factor, NupR, which, to our knowledge, is first GntR family factor primarily involved in regulation nucleoside transport.
Summary With the rapid development of synthetic biology in recent years, particular attention has been paid to RNA devices, especially riboswitches, because their significant and diverse regulatory roles prokaryotic eukaryotic cells. Due limited performance context‐dependence only a few them (such as theophylline, tetracycline ciprofloxacin riboswitches) have utilized tools biotechnology. In present study, we demonstrated that ribosome‐dependent ribo‐regulator, LRR, discovered our previous...