A5022834857- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- biodegradable polymer synthesis and properties
- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Lipid Membrane Structure and Behavior
- Bacterial biofilms and quorum sensing
- Probiotics and Fermented Foods
- Viral Infectious Diseases and Gene Expression in Insects
- Aquaculture disease management and microbiota
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Enzyme Catalysis and Immobilization
- Protein Structure and Dynamics
- Escherichia coli research studies
- Biopolymer Synthesis and Applications
- Cancer Research and Treatments
- Medicinal Plants and Neuroprotection
- Healthcare Operations and Scheduling Optimization
- Plant Pathogenic Bacteria Studies
- Glycosylation and Glycoproteins Research
- GABA and Rice Research
- Enzyme Production and Characterization
Jiangnan University
2015-2024
State Key Laboratory of Food Science and Technology
2016-2023
Lipopolysaccharides, major molecules in the outer membrane of Gram-negative bacteria, play important roles on integrity cell. However, how core oligosaccharide lipopolysaccharide affect behavior is not well understood. In this study, relationship between and was investigated using a series Escherichia coli mutants defective genes to biosynthesis lipopolysaccharide. Cell surface hydrophobicity, permeability, biofilm formation auto-aggregation these mutant cells were compared. Compared wild...
In metabolic engineering, unbalanced microbial carbon distribution has long blocked the further improvement in yield and productivity of high-volume natural metabolites. Current studies mostly focus on regulating desired biosynthetic pathways, whereas few strategies are available to maximize L-threonine efficiently. Here, we present a strategy guarantee supply reduced cofactors actualize maximization by cellular central pathways. A thermal switch system was designed applied divide whole...
Co-production of polyhydroxyalkanoate (PHA) and amino acids makes bacteria effective microbial cell factories by secreting outside while accumulating PHA granules inside. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is one the PHAs with biocompatibility fine mechanical properties, but its production limited low level intracellular propionyl-CoA.L-Isoleucine producing Corynebacterium glutamicum strain WM001 were analyzed genome transcriptome sequencing. The results showed that most...
Three genes, gnd, pgl, and fbp, relevant to the pentose phosphate pathway (PPP) were overexpressed in Corynebacterium glutamicum IWJ001, leading increase of l-isoleucine production. The transcriptional levels fbp significantly increased IWJ001/pDXW-8-gnd-fbp-pgl. Compared with control strain IWJ001/pDXW-8, intracellular NADPH/NADP+ ratios IWJ001/pDXW-8-gnd IWJ001/pDXW-8-gnd-fbp cells grown for 36 H threefold fourfold, respectively, indicating that overexpression gnd redirected carbon flux...
Flagella assembly was investigated in the Escherichia coli W3110 wild-type strain and ΔwaaF, ΔwaaC, ΔwaaG mutant strains that only synthesize lipopolysaccharide with different lengths, using transmission electron microscopy whole genome transcriptome profiling. Under microscope, flagella were observed on cell surface of but not or strains. Transcriptional profiling showed 1382 genes 526 965genes significantly regulated compared to control strain. These further analyzed by gene ontology KEGG...
Abstract Based on the analysis of sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and mass spectrometry, flagellin FliC a key member type VI secretion system Hcp1 were found to be secreted from Pseudomonas putida KT2442 cells in late exponential phase, but early phase only was secreted. Relevant genes fleQ rpoN then individually deleted P. KT2442, expression transcription fliC hcp1 these mutant strains analyzed. In both △ cells, not it even phase. Transcriptomic shows that most...
Gram-negative bacteria are intrinsically resistant to antibiotics due the presence of cell envelope, but mechanisms this resistance still not fully understood. In study, a series mutants that lack one or more major components associated with envelope were constructed from Escherichia coli K-12 W3110. WJW02 can only synthesize Kdo
Abstract l ‐Threonine is an important amino acid supplemented in food, medicine, or feed. Starting from glucose, ‐threonine production Escherichia coli involves the glycolysis, TCA cycle, and biosynthetic pathway. In this study, how producing E. TWF001 controlled by three regulators ArcA, Cra, IclR, which control expression of genes involved glycolysis has been investigated. Ten mutant strains were constructed different combinations deletion overexpression arcA , cra iclR tdcC . was...
The antibiotic resistance of the food-borne pathogen Vibrio parahaemolyticus has attracted researchers' attention in recent years, but its molecular mechanism remains poorly understood. In this study, 7 genes encoding outer membrane proteins (OMPs) were individually deleted V. ATCC33846, and these mutants to 14 antibiotics was investigated. results revealed that ampicillin significantly increased. Further exploration 20-gene transcription changes by real time-qPCR (RT-qPCR) demonstrated...
Poly-3-hydroxybutyrate is an environmentally friendly polymer with many promising applications and can be produced in Escherichia coli cells after overexpressing the heterologous gene cluster phaCAB. In this study, we found that truncating structure of lipopolysaccharide E. effectively enhance poly-3-hydroxybutyrate production. mutant strains WJW00, WJD00, WJJ00 were constructed by deleting rfaD from strain W3110, DH5α, JM109, respectively. Compared to controls W3110/pDXW-8-phaCAB,...
Abstract In this study, l ‐isoleucine production in Corynebacterium glutamicum WM001 was improved by deleting three genes the genome, replacing native promoter of ilvA and overexpression five an alr ‐based auxotrophic complementation expression system. The deleted genome are alaT , brnQ . Deletion increasing supply pyruvate, whereas deletion blocking uptake extracellular ‐isoleucine. Exchange with tac or tacM could contribute to 2‐ketobutyric acid; is better than for improving yield....
Colanic acid (CA) is exopolysaccharide that presents growing potential in the food and healthcare industry as a versatile polymer. Previously, we have constructed
2-Oxobutyrate (2-OBA), as a toxic metabolic intermediate, generally arrests the cell growth of most microorganisms and blocks biosynthesis target metabolites. In this study, we demonstrated that using acetate bypass to replace pyruvate dehydrogenase complex (PDHc) in Escherichia coli could recharge intracellular acetyl-CoA pool alleviate toxicity 2-OBA. Furthermore, based on crystal structure oxidase (PoxB), two candidate residues substrate-binding pocket PoxB were predicted by computational...
Understanding the detailed influence of OM on cell envelope and cellular metabolism is important for optimizing E. coli factory many other microorganisms. This study revealed applicability remodeling to enhance PHB accumulation as representative inclusion bodies.