A5089680280- Pancreatic function and diabetes
- Diabetes Management and Research
- Cannabis and Cannabinoid Research
- Diabetes and associated disorders
- 3D Printing in Biomedical Research
- Pancreatic and Hepatic Oncology Research
- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- Pancreatitis Pathology and Treatment
- Lymphoma Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Medical Imaging and Pathology Studies
- Mesenchymal stem cell research
- Microfluidic and Capillary Electrophoresis Applications
- Metabolism, Diabetes, and Cancer
- Electrohydrodynamics and Fluid Dynamics
- Genetic factors in colorectal cancer
- Immune Cell Function and Interaction
- Cancer Diagnosis and Treatment
- Congenital heart defects research
- Abdominal vascular conditions and treatments
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Endometrial and Cervical Cancer Treatments
- Biliary and Gastrointestinal Fistulas
University of California System
2025
City of Hope
2016-2024
University of California, San Francisco
2024
City Of Hope National Medical Center
2018-2023
Beckman Research Institute
2016-2022
California Institute of Technology
2018
Creative Commons
2018
Nora Eccles Treadwell Foundation
2018
Zero to Three
2016
Tokyo Women's Medical University
2014-2015
Type 1 diabetes is an autoimmune disease that destroys insulin-producing beta cells in the pancreas. Pancreatic islet transplantation could be effective treatment option for type once several issues are resolved, including donor shortage, prevention of necrosis and loss pre- post-transplantation, optimization immunosuppression. This study seeks to determine cause necrotic isolated islets improve transplant efficiency.The oxygen tension inside human different sizes was simulated under varying...
GNAS, a gene encoding G protein stimulating α subunit, is frequently mutated in intraductal papillary mucinous neoplasms (IPMNs), which are indolent and slow-growing pancreatic tumors that secrete abundant mucin. The GNAS mutation not observed conventional ductal adenocarcinomas of the pancreas. To determine functional significance lineage cells, we examined vitro phenotypes cells lineage, HPDE, PK-8, PCI-35, MIA PaCa-2, with exogenous expression either wild-type or (R201H) GNAS. We found...
Subcutaneous tissue is a promising site for islet transplantation, due to its large area and accessibility, which allows minimally invasive procedures graft monitoring, removal of malignancies as needed. However, relative the conventional intrahepatic transplantation site, subcutaneous requires number islets achieve engraftment success diabetes reversal, hypoxia low vascularity. We report that efficiency in Lewis rat model significantly improved by treating recipients with inhaled 50%...
Fireflies produce light through luciferase-catalyzed reactions involving luciferin, oxygen, and adenosine triphosphate, distinct from other luminescent organisms. This unique feature has revolutionized molecular biology physiology, serving as a valuable tool for cellular research. Luciferase-based bioluminescent imaging enabled the creation of transgenic animals, such Firefly Rats. Rats, created in 2006, ubiquitously express luciferase have become critical asset scientific investigations....
In clinical and experimental human pancreatic islet transplantations, establishing pretransplant assessments that accurately predict transplantation outcomes is crucial. Conventional in vitro viability assessment relies on manual counting of viable islets a routine assessment. However, this method does not correlate with outcomes; to improve the method, we recently introduced semi-automated using imaging software objectively determine area-based viability. The goal present study was...
Islet transplantation is an effective therapy that allows the achievement of insulin independence in patients with type 1 diabetes (T1D). To ensure successful transplantation, islet viability and function are great importance. Viability assessments most often use fluorescein diacetate (FDA)/propidium iodide (PI) staining. However, results using this method do not correlate well graft function. Because FDA nonspecifically penetrates all cells present preparation, including islets...
Umbilical cord mesenchymal stem cell-derived extracellular vesicles (UC-MSC-EVs) have become an emerging strategy for treating various autoimmune and metabolic disorders, particularly diabetes. Delivery of UC-MSC-EVs is essential to ensure optimal efficacy UC-MSC-EVs. To develop safe superior EVs-based delivery strategies, we explored nuclear techniques including positron emission tomography (PET) evaluate the in vivo. In this study, human were first successfully tagged with I-124 permit PET...
Islet transplantation is a promising treatment for type-1 diabetes; however, donor shortage concern. Even when pancreas available, low islet yield limits the success of transplantation. culture enables pooling multiple low-yield isolations into an effective mass, but isolated islets rapidly deteriorate under conventional conditions. Oxygen (O2) depletion in core, which leads to central necrosis and volume loss, one major reasons this deterioration.To promote long-term human PIM-R medium...
Prior to transplantation into individuals with type 1 diabetes, in vitro assays are used evaluate the quality, function and survival of isolated human islets. In addition assessments these parameters islet, they can be evaluated by multiparametric morphological scoring (0–10 points) grading (A, B, C, D, F) based on islet characteristics (shape, border, integrity, single cells, diameter). However, correlation between assessment outcome has not been fully elucidated. this study, 55 isolations...
Porous microwell scaffolds of biodegradable polymers gelatin and poly(lactic- co -glycolic acid) (PLGA) were prepared for 3D culture pancreatic beta cells to promote the formation cell aggregates insulin secretion.
The need for maintaining cell-spheroid viability and function within high-density cultures is unmet various clinical experimental applications, including cell therapies. One immediate application transplantation of pancreatic islets, a clinically recognized treatment option to cure type 1 diabetes; islets are isolated from donor subsequent culture prior transplantation. However, high seeding conditions cause unsolicited fusion multiple spheroids, thereby limiting oxygen diffusion induce...
Objective Islet transplantation is an effective treatment for type 1 diabetes. However, transplant success depends on quick islet assessment because islets deteriorate 2–3 days after isolation. A new tool, single-cell western blot (scWestern), offers results within day. In this study, we aimed to test the suitability of scWestern detect protein markers beta (insulin), alpha (glucagon), and delta (somatostatin) cells, 3 major endocrine cell types in islets. Materials Methods We characterized...
Cell transplantation is a promising treatment for complementing lost function by replacing new cells with desired function, e.g. pancreatic islet diabetics. To prevent cell obliteration, oxygen supply critical after transplantation, especially until the graft sufficiently re-vascularized. during this period, we developed chemical-/electrical-free implantable transporter that delivers to hypoxic site from ambient air diffusion potential. This device simply structured using biocompatible...
Defining the oxygen level that induces cell death within 3-D tissues is vital for understanding tissue hypoxia; however, obtaining accurate measurements has been technically challenging. In this study, we introduce a noninvasive, high-throughput methodology to quantify critical survival partial pressure (pO