A5052144423- Pancreatitis Pathology and Treatment
- Pancreatic and Hepatic Oncology Research
- Pancreatic function and diabetes
- Phagocytosis and Immune Regulation
- Autophagy in Disease and Therapy
- Neuroendocrine Tumor Research Advances
- Cancer Genomics and Diagnostics
- Liver Disease Diagnosis and Treatment
- Endoplasmic Reticulum Stress and Disease
- Gastrointestinal disorders and treatments
- CRISPR and Genetic Engineering
- Occupational and environmental lung diseases
- Oral and gingival health research
- Blood Coagulation and Thrombosis Mechanisms
- Digestive system and related health
- Pediatric Hepatobiliary Diseases and Treatments
- Cell death mechanisms and regulation
- Abdominal Surgery and Complications
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Animal Genetics and Reproduction
- Lung Cancer Research Studies
- Colorectal Cancer Treatments and Studies
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Acute Kidney Injury Research
Hyogo University
2022-2025
Osaka University
2021-2022
Toho University
2022
Kyoto University
2022
Hyogo Medical University
2016-2021
Mesothelioma Applied Research Foundation
2018
Takeda (Japan)
2018
Eisai (United Kingdom)
2018
Kumamoto University
2008-2017
Ehime University
2009
Autophagy is mostly a nonselective bulk degradation system within cells. Recent reports indicate that autophagy can act both as protector and killer of the cell depending on stage disease or surrounding cellular environment (for review see Cuervo, A.M. 2004. Trends Cell Biol. 14:70–77). We found cytoplasmic vacuoles induced in pancreatic acinar cells by experimental pancreatitis were autophagic origin, demonstrated microtubule-associated protein 1 light chain 3 expression electron microscopy...
We hypothesized that four criteria could help identify malignant mesotheliomas (MMs) most likely linked to germline mutations of BAP1 or other genes: family history MM, BAP1-associated cancers, multiple malignancies; age younger than 50 years.
Serine protease inhibitor, Kazal type 1 (SPINK1) is expressed not only in normal human pancreatic acinar cells but also a variety of ductal neoplasms. There are structural similarities between SPINK1 and epidermal growth factor (EGF). Hence, we hypothesized that binds to EGF receptor (EGFR) activate its downstream signaling. We first showed induced proliferation NIH 3T3 cancer cell lines. coprecipitated with EGFR an immunoprecipitation experiment the binding affinity was about half using...
The antiapoptotic protein c-FLIP blocks multiple cell death pathways in mice.
To evaluate the clinical applicability of determination disseminated intravascular coagulation (DIC) parameters in acute pancreatitis.The subjects for this study were 139 consecutive patients with pancreatitis. DIC assessed at initial observation these patients.The levels admission significantly associated severity and prognosis Antithrombin III (AT-III), fibrin/fibrinogen degradation products-E, platelet count, D-dimer, thrombin-AT-III complex showed better area under receiver operating...
Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome editing permits the rapid production of genetically engineered mice. To make most this innovative technology, a streamlined procedure is needed for robust construction CRISPR/Cas9 vectors, efficient preparation mouse oocytes, and refined genotyping strategies. Although we previously demonstrated applicability oocyte cryopreservation technologies various methods in...
Autodigestion of the pancreas by its own prematurely activated digestive proteases is thought to be an important event in onset acute pancreatitis. Although lysosomal hydrolases, such as cathepsin B, play a key role intrapancreatic trypsinogen activation, it remains unclear where and how meets these enzymes. Autophagy intracellular bulk degradation system which cytoplasmic components are directed lysosome/vacuole membrane-mediated process. To analyze autophagy pancreatitis, we produced...
Activating Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations are the most common and frequent changes observed in pancreatic cancer. This study aimed to determine frequency extent of intratumoral metastatic lymph node KRAS mutation heterogeneity resected ductal adenocarcinoma.Tumor tissues macrodissected from tumor centers, invasion fronts (n = 97), nodes 11) were subjected DNA extraction analysis codons 12 13 by pyrosequencing.Activating codon detected 90 (92.8%) centers. No any...
Abstract Thymic selection shapes an appropriate T cell antigen receptor (TCR) repertoire during development. Here, we show that a serine/threonine kinase, protein kinase D (PKD), is crucial for thymocyte positive selection. In cell-specific PKD-deficient (PKD2/PKD3 double-deficient) mice, the generation of CD4 single thymocytes abrogated. This defect likely caused by attenuated TCR signalling and incomplete lineage specification in thymocytes; however, TCR-proximal tyrosine phosphorylation...
The major lysosomal proteases, Cathepsin B (CTSB), D (CTSD) and L (CTSL), are implicated in autophagic activity. To investigate the role of each cathepsin exocrine pancreas, we generated mice which pancreas was specifically deficient Ctsb, Ctsd Ctsl. Each these gene knockout (KO) Ctsb;Ctsl Ctsd;Ctsl double-knockout (DKO) were almost normal. However, found cytoplasmic degeneration pancreatic acinar cells Ctsb;Ctsd DKO mice, similar to autophagy related 5 (Atg5) KO mice. LC3 p62 (autophagy...
Introduction Severe acute pancreatitis is occasionally associated with pancreatic and intestinal necrosis. Mesenteric vasoconstriction one of the most probable types pathogenesis these complications. Aim To investigate involvement endothelin-1 (ET-1), a potent vasoconstrictor. Methodology Results Plasma ET-1 concentrations were extremely high in patients and/or diffuse mRNA was demonstrated rat pancreas, production protein by human umbilical vein endothelial cells enhanced tumor necrosis...
Studies on hereditary pancreatitis have provided evidence in favor of central role for trypsin activity the disease. Identification genetic variants trypsinogen linked protease to onset pancreatitis, and biochemical characterization proposed an enzymatic gain function as initiating mechanism. Mutations serine inhibitor Kazal type 1gene (SPINK1) are shown be associated with pancreatitis. We previously reported that Spink3 (a mouse homologue gene human SPINK1) deficient mice showed excessive...
Background: The aim of this study was to assess the effects postoperative body weight loss on long‐term survival after resection for pancreatic cancer. Methods : A total 93 patients with primary cancer underwent between April 2005 and December 2011. Patient characteristics, preoperative mass index, changes in were evaluated retrospectively correlated survival. Results There no significant association index. Body fell by 8.4% at 2 months surgery 9.0% 4 surgery. Severe losses, both ( P = .033)...
Genome editing with site-specific nucleases, such as zinc-finger nucleases or transcription activator-like effector (TALENs), and RNA-guided the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system, is becoming new standard for targeted genome modification in various organisms. Application of these techniques to manufacture knockout mice would be greatly aided by simple easy methods genotyping mutant wild-type pups among litters. However, there are...
Colorectal cancer is a major cause of deaths due to cancer; therefore, research into its etiology urgently needed. Although it clear that chronic inflammation risk factor for colorectal cancer, the details remain uncertain. Serine protease inhibitor, Kazal type 1 (SPINK1) mainly produced in pancreatic acinar cells. However, SPINK1 expressed various cancers and inflammatory states, such as colon bowel disease. There are structural similarities between epidermal growth (EGF). Hence, was...