A5004840019- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Zebrafish Biomedical Research Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Immune cells in cancer
- Viral Infections and Immunology Research
- Physiological and biochemical adaptations
- Streptococcal Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Pluripotent Stem Cells Research
- Gene Regulatory Network Analysis
- Cancer-related molecular mechanisms research
- Acute Myeloid Leukemia Research
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Neonatal Respiratory Health Research
- Developmental Biology and Gene Regulation
- Bacterial Identification and Susceptibility Testing
- High Altitude and Hypoxia
- RNA Interference and Gene Delivery
Hospital del Mar Research Institute
2013-2015
Barcelona Biomedical Research Park
2013-2014
Universidade Nova de Lisboa
2005-2011
Instituto de Biologia Experimental e Tecnológica
2006-2011
Helmholtz Centre for Infection Research
2007-2011
Abstract Acquisition of the arterial and haemogenic endothelium fates concurrently occur in aorta–gonad–mesonephros (AGM) region prior to haematopoietic stem cell (HSC) generation. The programme depends on Dll4 endothelium/HSC Jag1-mediated Notch1 signalling. How distinguishes executes these different programmes response particular ligands is poorly understood. By using two activation trap mouse models with sensitivity, here we show that endothelial cells HSCs originate from distinct...
Hematopoietic stem cell (HSC) specification occurs in the embryonic aorta and requires Notch activation; however, most of Notch-regulated elements controlling de novo HSC generation are still unknown. Here, we identify putative direct targets aorta-gonad-mesonephros (AGM) tissue by chromatin precipitation using antibodies against partner RBPj. By ChIP-on-chip analysis precipitated DNA, identified 701 promoter regions that were candidates to be regulated AGM. One enriched corresponded Cdca7...
Abstract Background Recombinant protein expression in mammalian cells is mostly achieved by stable integration of transgenes into the chromosomal DNA established cell lines. The surroundings have strong influences on transgenes. exploitation defined loci targeting constructs with different regulatory elements an approach to design high level systems. Further, this allows evaluate impact distinct vector constructs. Results We explored antibody upon diverse previously tagged CHO-K1 and HEK293...
Currently, lentiviral vectors for research and gene therapy are produced from 293-T cells that transiently transfected with plasmids encoding the vector helper functions. However, as well presence of SV40 virus large T-antigen (T-Ag) cause serious technical safety considerations. We aimed to exploit single copy integration sites in HEK293 genome supporting production. found result minimal infectious particle production integrants HEK293. Moreover, once this cell line harbors integrations...
In this study, we analyzed the antimicrobial resistance properties and T antigenic types of 511 isolates collected in Lisbon district, Portugal, from throat swabs healthy subjects (n=341), during 2000-2002 diverse infection sites (n=170) outpatients inpatients, 1999-2002. Erythromycin was higher tonsillitis/pharyngitis (27.4%) skin (21.1%), than carriage invasive (<or=10%). Differences erythromycin among children adults were noticed only for (9.3% 21.1% adults). Most erythromycin-resistant...
The improvement of safety and titer retroviral vectors produced in standard packaging cell lines is hampered because production relies on uncontrollable vector integration events. influences chromosomal surroundings make it difficult to dissect the performance a specific from respective site. Taking advantage technology that use with predefined sites, we have systematically evaluated several vectors. In two previously established modular (Flp293A 293 FLEX) single, defined were integrated by...
The strength of recombinant gene expression is a key property cell lines for biopharmaceutical protein production. In most stable the vector stably introduced into host chromosomal DNA. Apart from copy number and used control elements performance vectors modulated by genetic epigenetic features provided flanking elements. Since targeted integration very difficult clones with high are created random large scale screening expression. This allows isolation those rare cells in which optimal....
generation and both lineages share a common endothelial progenitor (Zovein et al., 2008;Chen 2009).The process by which an HSC precursor with characteristic acquires the hematopoietic identity is known as to transition.HSCs develop within specific cell clusters budding from endothelium lumen of dorsal aorta in region comprised between junctions vitellin umbilical arteries (Yokomizo Dzierzak, 2010).These