A5035890683- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Insect Resistance and Genetics
- Iron Metabolism and Disorders
- Animal Genetics and Reproduction
- Neurobiology and Insect Physiology Research
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- interferon and immune responses
- RNA modifications and cancer
- RNA regulation and disease
- Mosquito-borne diseases and control
- Chemical synthesis and alkaloids
- Plant Molecular Biology Research
- Folate and B Vitamins Research
- Viral Infections and Immunology Research
- Chromosomal and Genetic Variations
- Genetics, Aging, and Longevity in Model Organisms
- Porphyrin Metabolism and Disorders
- Cholinesterase and Neurodegenerative Diseases
University of Alberta
2018-2024
Specific Technologies (United States)
2024
University of Waterloo
2022
Murray State University
2016
Abstract Advances in CRISPR technology have immensely improved our ability to manipulate nucleic acids, and the recent discovery of RNA-targeting endonuclease Cas13 adds even further functionality. Here, we show that works efficiently Drosophila , both ex vivo vivo. We test 44 different variants identify enzymes with best overall performance could target endogenous transcripts high efficiency specificity. also develop applications edit mRNAs mitochondrial transcripts. Our vector collection...
Abstract Iron Regulatory Protein 1 (IRP1) is a bifunctional cytosolic iron sensor. When levels are normal, IRP1 harbours an iron-sulphur cluster (holo-IRP1), enzyme with aconitase activity. fall, loses the (apo-IRP1) and binds to iron-responsive elements (IREs) in messenger RNAs (mRNAs) encoding proteins involved cellular uptake, distribution, storage. Here we show that mutations Drosophila 1,4-Alpha-Glucan Branching Enzyme ( AGBE ) gene cause porphyria. was hitherto only linked glycogen...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has made it clear that combating outbreaks benefits from a combination of vaccines and therapeutics. A promising drug target common to all coronaviruses—including SARS-CoV, MERS-CoV, SARS-CoV-2—is the papain-like protease (PLpro). PLpro cleaves part viral replicase polyproteins into non-structural protein subunits, which are essential replication cycle. Additionally, can cleave both ubiquitin ubiquitin-like ISG15 host...
The final body size of any given individual underlies both genetic and environmental constraints. Both mammals insects use target rapamycin (TOR) insulin signaling pathways to coordinate growth with nutrition. In holometabolous insects, the period is terminated through a cascade peptide steroid hormones that end larval feeding behavior trigger metamorphosis, nonfeeding stage during which plan remodeled produce an adult. This irreversible decision, termed critical weight (CW) checkpoint,...
Abstract Targeting gene function with spatial or temporal specificity is a key goal in molecular genetics. CRISPR-Cas9 has greatly facilitated this strategy, but some standard approaches are problematic. For instance, simple tissue-specific global overexpression of Cas9 can cause significant lethality developmental delays even the absence gRNAs. In particular, we found that Gal4-mediated expression UAS-Cas9 Drosophila prothoracic gland (PG) was not suitable strategy to disrupt expression,...
Macromolecular complexes and organelles play crucial roles within cells, but their native architectures are often unknown. Here, we use an evolutionarily conserved germline organelle, the germ granule, as a paradigm. In Drosophila embryos, map one of its interactomes using novel in vivo crosslinking approach that employs two interacting granule proteins determines common neighbor molecules. We identified assembly Tudor, Aubergine, motor metabolic proteins, RNA helicases, provide evidence for...
ABSTRACT Recent studies have revealed a role for RNA in the repair of DNA double-strand breaks. Here, we show that asymmetric overhangs generated by small TevSaCas9 dual nuclease informs simple and robust editing strategy human cells whereby Polθ Rad52 are recruited to break. The 2-nt, 3’ overhang I-TevI domain hybridizes with end co-localized template guide specifically license repair. Substitutions destabilize duplex reduce efficiency. Targeted RNA-templated (rep-editing) harnesses...
Abstract Intravenous injection provides a direct, rapid, and efficient route for delivering drugs or other substances, particularly compounds with poor intestinal absorption molecules (e.g. proteins) that are prone to structural changes degradation within the digestive system. While Drosophila larvae represent well-established genetic model studying developmental physiological pathways, as well human diseases, their use in analyzing molecular effects of substance exposure remains limited. In...
Abstract Targeting gene function with spatial or temporal specificity is a key goal in molecular genetics. CRISPR-Cas9 has greatly facilitated this strategy, but some standard approaches are problematic. For instance, simple tissue-specific global overexpression of Cas9 can cause significant lethality developmental delays even the absence gRNAs. In particular, we found that Gal4-mediated expression UAS-Cas9 Drosophila prothoracic gland (PG) was not suitable strategy to disrupt expression,...
Summary Advances in CRISPR technology have immensely improved our ability to manipulate nucleic acids, and the recent discovery of RNA-targeting endonuclease Cas13 adds even further functionality. Here, we show that works efficiently Drosophila , both ex vivo . We tested 44 different variants identify enzymes with best overall performance showed could target endogenous transcripts high efficiency specificity. also developed applications edit mRNAs mitochondrial transcripts. Our vector...