A5082711409- Genomics, phytochemicals, and oxidative stress
- Moringa oleifera research and applications
- Free Radicals and Antioxidants
- Garlic and Onion Studies
- Nitrogen and Sulfur Effects on Brassica
- Phytochemicals and Antioxidant Activities
- Plant pathogens and resistance mechanisms
- Agronomic Practices and Intercropping Systems
- Selenium in Biological Systems
- Plant Stress Responses and Tolerance
- Glutathione Transferases and Polymorphisms
- Agriculture, Plant Science, Crop Management
- Light effects on plants
- Plant-Microbe Interactions and Immunity
- Biochemical Acid Research Studies
- Vitamin C and Antioxidants Research
- Postharvest Quality and Shelf Life Management
- Plant-derived Lignans Synthesis and Bioactivity
- Essential Oils and Antimicrobial Activity
- Energy and Environment Impacts
- Seed and Plant Biochemistry
- Plant Physiology and Cultivation Studies
- Polyamine Metabolism and Applications
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
Leibniz Institute of Vegetable and Ornamental Crops
2016-2025
Technische Universität Berlin
2011-2025
Institute of Food Science and Technology
2025
Leibniz-Institute for Food Systems Biology at the Technical University of Munich
2025
University of Potsdam
2022
German Institute of Human Nutrition
2022
Leibniz Association
2014-2015
Leibniz Institute for Neurobiology
2014
Vegetables of the Brassica oleracea group, such as broccoli, cauliflower, and cabbage, play an important role for glucosinolate consumption in human diet. Upon maceration vegetable tissue, glucosinolates are degraded enzymatically to form volatile isothiocyanates, nitriles, epithionitriles. However, only uptake isothiocyanates is linked cancer-preventive effects. Thus, it great interest evaluate especially isothiocyanate formation. Here, we studied formation their respective hydrolysis...
From the order Brassicales, different plant organs, such as inflorescences (e.g. broccoli and cauliflower), leaves kale pak choi), heads white red cabbage), well roots bulbs radish turnip), are frequently consumed brassicaceaous vegetables. The Brassicales is characterized by a specific group of secondary metabolites, namely glucosinolates. Glucosinolates their breakdown products isothiocyanates linked to conferring beneficial health effects. In addition, some studies have also highlighted...
Abstract Consumption of glucosinolate-rich Brassicales vegetables is associated with a decreased risk cancer enzymatic hydrolysis glucosinolates playing key role. However, formation health-promoting isothiocyanates inhibited by the epithiospecifier protein in favour nitriles and epithionitriles. Domestic processing conditions, such as changes pH value, temperature or dilution, might also affect isothiocyanate formation. Therefore, influences these three factors were evaluated accessions...
Plant metabolite profiling reveals the diversity of secondary or specialized metabolites in plant kingdom with its hundreds thousands species. Specialized constitute a vast class chemicals posing significant challenges analytical chemistry. In order to be maximum scientific relevance, reports dealing these compounds and their source species must transparent, make use standards reference materials, based on correctly traceably identified material. Essential aspects qualitative include: (i)...
Glucosinolates, present in Brassica vegetables, are thought to contribute human health prevention because of their enzymatically induced breakdown products, primarily isothiocyanates (ITCs). ITCs reactive substances that readily react with nucleophilic (food) compounds. The reactivity allyl-ITC and 4-(methylsulfinyl)butyl-ITC (sulforaphane) toward thiol amino groups cysteine lysine derivatives was studied buffered model systems as well broccoli sprouts. group is the preferred reaction site,...
Domestic processing methods, such as boiling, significantly affect the glucosinolate content and formation of breakdown products in Brassica vegetables. Here, we comprehensively describe effect aqueous heat treatment on degradation leaching kinetics glucosinolates their enzymatic non-enzymatic hydrolysis products. The results were correlated with inactivation myrosinase epithiospecifier protein activity oleracea vegetables kohlrabi, white cabbage, red cabbage. Short-term heating increased...
Pak choi plants (Brassica rapa ssp. chinensis) were treated with different signaling molecules methyl jasmonate, jasmonic acid, linolenic and salicylate analyzed for specific changes in their glucosinolate profile. Glucosinolate levels quantified using HPLC-DAD-UV, focus on induction of indole glucosinolates special emphasis 1-methoxy-indol-3-ylmethyl glucosinolate. Furthermore, the effects accumulation level gene expression semi-quantitative realtime RT-PCR selected genes. The treatments...
Brassicales species rich in glucosinolates are used for biofumigation, a process based on releasing enzymatically toxic isothiocyanates into the soil. These hydrolysis products volatile and often reactive compounds. Moreover, can be degraded also without presence of hydrolytic enzyme myrosinase which might contribute to bioactive effects. Thus, present study stability Brassicaceae plant-derived pure was studied using three different soils (model biofumigation). In addition, degradation...
Pak choi (Brassica rapa subsp. chinensis) is rich in secondary metabolites and contains numerous antioxidants, including flavonoids; hydroxycinnamic acids; carotenoids; chlorophylls; glucosinolates, which can be hydrolyzed to epithionitriles, nitriles, or isothiocyanates. Here, we investigate the effect of reduced exposure ultraviolet B (UVB) UV (UVA UVB) light at four different developmental stages pak choi. We found that both plant morphology metabolite profiles were affected by UVB UV,...
Processing reduces the glucosinolate (GSL) content of plant food, among other aspects due to thermally induced degradation. Since there is little information about thermal stability GSL and formation corresponding breakdown products, degradation sulfur-containing aliphatic was studied in broccoli sprouts with isolated dry medium at different temperatures as well aqueous pH values. Desulfo-GSL have been analyzed HPLC-DAD, while products were estimated using GC-FID. Whereas structural...
Besides glucosinolates, Brassica vegetables accumulate sulfur-containing (+)-S-methyl-l-cysteine sulfoxide (SMCSO, methiin), mainly known from Allium vegetables. Such (+)-S-alk(en)yl-l-cysteine sulfoxides can degrade to volatile organosulfur compounds (VOSCs), which have been linked health beneficial effects. In the present study, accumulation of SMCSO and formation VOSCs was investigated in oleracea content commercially available white red cabbages monitored over a three-month period with...
In Brassica vegetables, heating processes lead to thermally induced degradation of glucosinolates (GSLs), resulting in the formation nitriles and isothiocyanates (ITCs). To date, mechanism is not yet satisfyingly elucidated. Thermally model GSL sinigrin was studied dry as well aqueous medium at different pH values temperatures. The influence presence iron ions plant matrix (broccoli sprouts powder) on well. Next GSL, nitrile ITC release sugar derivatives were investigated. Because d-glucose...
Background: Ethiopian kale (Brassica carinata) is a horticulturally important crop used as leafy vegetable in large parts of East and Southern Africa. The leaves are reported to contain high concentrations health-promoting secondary plant metabolites. However, scientific knowledge on their health benefits scarce. Objective: This study aimed determine the cancer preventive potential B. carinata using human liver vitro model focusing processing effects pattern metabolites bioactivity. Design:...
Scope Glucosinolates are secondary metabolites present in Brassica vegetables. Alkenyl glucosinolates enzymatically degraded forming nitriles or isothiocyanates, but the presence of epithiospecifier protein, epithionitriles released. However, studies on occurrence food and knowledge about their biological effects scarce. Methods results Epithionitrile formation from seven vegetables was analyzed using GC‐MS HPLC‐DAD. Bioactivity synthetic plant‐derived 1‐cyano‐2,3‐epithiopropane (CETP) –...
Casparian strip-generated apoplastic barriers not only control the radial flow of both water and ions but may also constitute a hindrance for rhizosecretion stele-synthesized phytochemicals. Here, we establish root-synthesized glucosinolates (GLS) are in Arabidopsis as model to study transport routes plant-derived metabolites from site synthesis rhizosphere. Analysing expression GLS synthetic genes root indicate that stele is major aliphatic GLS, whereas indole can be synthesized cortex....
An in-depth glucosinolate (GLS) profiling was performed on a core collection of 91 Brassica rapa accessions, representing diverse morphotypes heterogeneous geographical origin, to better understand the natural variation in GLS accumulation and breakdown product formation. Leaves B. accessions were analyzed for their composition by UHPLC-DAD corresponding products GC-MS. Fifteen different GLSs identified, aliphatic prevailed regarding diversity concentration. Twenty-three among them nine...
Rocket is rich in glucosinolates and valued for its hot spicy taste. Here we report the structure elucidation, bioactivity, stability of mainly formed glucosinolate hydrolysis product, namely sativin, which was formerly thought to be 4-mercaptobutyl isothiocyanate. However, by NMR characterization revealed that sativin fact 1,3-thiazepane-2-thione, a tautomer isothiocyanate with 7-membered ring so far unknown. This finding further substantiated conformation sampling using molecular modeling...