A5090756369- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Genomics and Chromatin Dynamics
- Alcohol Consumption and Health Effects
- RNA Research and Splicing
- Cancer, Lipids, and Metabolism
- RNA and protein synthesis mechanisms
- Kidney Stones and Urolithiasis Treatments
- Pancreatic function and diabetes
- Genomics and Phylogenetic Studies
- Analytical Chemistry and Chromatography
- Molecular Biology Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Adipose Tissue and Metabolism
- Lipid metabolism and disorders
- Liver physiology and pathology
- Machine Learning in Bioinformatics
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Diet, Metabolism, and Disease
- Lipid metabolism and biosynthesis
- Epigenetics and DNA Methylation
- MRI in cancer diagnosis
- Glycosylation and Glycoproteins Research
- Sphingolipid Metabolism and Signaling
TU Dortmund University
2014-2023
Leibniz Research Centre for Working Environment and Human Factors
2014-2023
University of Münster
2012
The binding and contribution of transcription factors (TF) to cell specific gene expression is often deduced from open-chromatin measurements avoid costly TF ChIP-seq assays. Thus, it important develop computational methods for accurate prediction in regions (OCRs). Here, we report a novel segmentation-based method, TEPIC, predict by combining sets OCRs with position weight matrices. TEPIC can be applied various data, e.g. DNaseI-seq NOMe-seq. Additionally, Histone-Marks (HMs) used identify...
It is well known that isolation and cultivation of primary hepatocytes cause major gene expression alterations. In the present genome-wide, time-resolved study cultivated human mouse hepatocytes, we made observation changes in culture strongly resemble alterations liver diseases. Hepatocytes both species were collagen sandwich monolayer conditions. Genome-wide data also obtained from NAFLD, cirrhosis, HCC hepatitis B virus-infected tissue as livers after partial hepatectomy, CCl4...
Epigenetic modifications (e.g. DNA methylation) in NAFLD and their contribution to disease progression extrahepatic complications are poorly explored. Here, we use an integrated epigenome transcriptome analysis of mouse hepatocytes identify alterations glyoxylate metabolism, a pathway relevant kidney damage via oxalate release-a harmful waste product stone-promoting factor. Downregulation hypermethylation alanine-glyoxylate aminotransferase (Agxt), which detoxifies glyoxylate, preventing...
Non-alcoholic steatohepatitis (NASH) represents a risk factor for the development of hepatocellular carcinoma (HCC) and is characterized by quantitative qualitative changes in hepatic lipids. Since elongation fatty acids from C16 to C18 has recently been reported promote both lipid accumulation inflammation we aimed investigate whether frequently used mouse NASH model reflects this clinically relevant feature can be observed HCC development. Feeding mice methionine choline deficient diet not...
Endothelial lipase (LIPG) is a cell surface associated that displays phospholipase A1 activity towards phosphatidylcholine present in high-density lipoproteins (HDL). LIPG was recently reported to be expressed breast cancer and support proliferation, tumourigenicity metastasis. Here we show severe oxidative stress leading AMPK activation triggers upregulation, resulting intracellular lipid droplet accumulation cells, which supports survival. Neutralizing abrogated upregulation the...
High‐resolution magic angle spinning (HR MAS) nuclear magnetic resonance (NMR) spectroscopy is increasingly being used to study metabolite levels in human breast cancer tissue, assessing, for instance, correlations with prognostic factors, survival outcome or therapeutic response. However, the impact of intratumoral heterogeneity on tumor tissue has not been studied comprehensively. More specifically, when biopsy material analyzed, it remains questionable whether one representative entire...
Abstract E- and P-selectin ligands (E- P-ligs) guide effector memory T cells into skin inflamed regions, mediate the inflammatory recruitment of leukocytes, contribute to localization hematopoietic precursor cells. A better understanding their molecular regulation is therefore significant interest with regard therapeutic approaches targeting these pathways. In this study, we examined transcriptional fucosyltransferase 7 (FUT7), an enzyme crucial for generation glycosylated P-ligs. We found...
Abstract Shiga toxins (Stxs) are composed of an enzymatically active A subunit (StxA) and a pentameric B (StxB) that preferentially binds to the glycosphingolipid (GSL) globo\xadtriaosylceramide (Gb3Cer/CD77) reduced extent globotetraosylceramide (Gb4Cer). The identification Gb3Cer as tumor-associated GSL in human pancreatic cancer prompted us investigate expression Gb4Cer 15 ductal adenocarcinoma cell lines derived from primary tumors liver, ascites, lymph node metastases. Thin-layer...
Abstract ChIP-seq probes genome-wide localization of DNA-associated proteins. To mitigate technical biases read densities are normalized to obtained by a control. Our statistical framework “normR” achieves sensitive normalization accounting for the effect putative protein-bound regions on overall statistics. Here, we demonstrate normR’s suitability in three studies: (i) calling enrichment high (H3K4me3) and low (H3K36me3) signal-to-ratio data; (ii) identifying two previously undescribed...
Abstract The binding and contribution of transcription factors (TF) to cell specific gene expression is often deduced from open-chromatin measurements avoid costly TF ChIP-seq assays. Thus, it important develop computational methods for accurate prediction in regions (OCRs). Here, we report a novel segmentation-based method, TEPIC, predict by combining sets OCRs with position weight matrices. TEPIC can be applied various data, e.g. DNaseI-seq NOMe-seq. Additionally, Histone-Marks (HMs) used...
Fatty liver disease or the accumulation of fat in liver, has been reported to affect global population. This comes with an increased risk for development fibrosis, cirrhosis, and hepatocellular carcinoma. Yet, little is known about effects a diet containing high alcohol towards epigenetic aging, respect changes transcriptional epigenomic profiles. In this study, we took up multi-omics approach integrated gene expression, methylation signals, chromatin signals study high-fat...
Non-alcoholic fatty liver disease (NAFLD) encompasses a wide range of diseases, where excessive hepatic lipid accumulation is common factor. It highly prevalent worldwide, and associated with an elevated risk developing more severe as well cardiovascular renal such the formation kidney stones. The aim this study was to identify investigate lipid-associated metabolic functional alterations in steatotic livers hepatocytes. For purpose, mouse model NAFLD, leptin deficient ob/ob mouse,...
Epigenetic modifications (e. g. DNA methylation) in NAFLD and their contribution to disease progression extrahepatic complications are poorly explored. Here, we use an integrated epigenome transcriptome analysis of mouse hepatocytes identify alterations glyoxylate metabolism, a pathway relevant kidney damage via oxalate release - harmful waste product stone-promoting factor. Downregulation hypermethylation alanine-glyoxylate aminotransferase (Agxt), which detoxifies glyoxylate, preventing...