A5083916533- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Metabolomics and Mass Spectrometry Studies
- Analytical chemistry methods development
- Advanced Chemical Sensor Technologies
- Microbial Metabolic Engineering and Bioproduction
- Effects and risks of endocrine disrupting chemicals
- Microbial bioremediation and biosurfactants
- Pesticide Residue Analysis and Safety
- Bacterial Genetics and Biotechnology
- Advanced Proteomics Techniques and Applications
- Lipid metabolism and biosynthesis
- Cancer, Lipids, and Metabolism
- Plant Micronutrient Interactions and Effects
- Cancer, Hypoxia, and Metabolism
- Insect and Pesticide Research
- Trace Elements in Health
- Skin Protection and Aging
- Animal testing and alternatives
- Toxic Organic Pollutants Impact
- Peroxisome Proliferator-Activated Receptors
- Pharmacogenetics and Drug Metabolism
- Electrochemical Analysis and Applications
- Analytical Chemistry and Sensors
- Indoor Air Quality and Microbial Exposure
University of Münster
2016-2025
Leibniz Institute for Analytical Sciences - ISAS
2006-2013
University of Wuppertal
2011-2013
Kirchhoff (Germany)
2006-2011
Leibniz Institute for Neurobiology
2010
TU Dortmund University
2006-2009
Institut des Sciences Analytiques
2008
University of Twente
2002-2005
Institute for Marine Biosciences
2005
Abstract Molecular networking connects mass spectra of molecules based on the similarity their fragmentation patterns. However, during ionization, commonly form multiple ion species with different behavior. As a result, these often remain unconnected in tandem spectrometry-based molecular networks, leading to redundant and disconnected sub-networks same compound classes. To overcome this bottleneck, we develop Ion Identity Networking (IIMN) that integrates chromatographic peak shape...
The peroxisome represents a ubiquitous single membrane-bound key organelle that executes various metabolic pathways such as fatty acid degradation by α- and β-oxidation, ether-phospholipid biosynthesis, metabolism of reactive oxygen species, detoxification glyoxylate in mammals. To fulfil this vast array functions, peroxisomes accommodate ∼50 different enzymes at least identified until now. Interest has been fueled the discovery group genetic diseases humans, which are caused either defect...
Rhamnolipids are biosurfactants featuring surface-active properties that render them suitable for a broad range of industrial applications. These include their emulsification and foaming capacity, critical micelle concentration, ability to lower surface tension. Further, aspects like biocompatibility environmental friendliness becoming increasingly important. mainly produced by pathogenic bacteria Pseudomonas aeruginosa. We previously designed constructed recombinant putida KT2440, which...
The peroxisome is a key organelle of low abundance that fulfils various functions essential for human cell metabolism. Severe genetic diseases in humans are caused by defects biogenesis or deficiencies the function single peroxisomal proteins. To improve our knowledge this important cellular structure, we studied first time liver peroxisomes quantitative proteomics. Peroxisomes were isolated differential and Nycodenz density gradient centrifugation. A label-free study 314 proteins across was...
Abstract To broaden the substrate scope of microbial cell factories towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE). However, comprehensive studies enabling a holistic understanding adaptation processes primed by metabolic engineering remain scarce. The industrial workhorse Pseudomonas putida was engineered to utilize non-native sugar D-xylose, but its assimilation into bacterial biochemical network via exogenous xylose...
Based on the ability of phytosiderophores to chelate other heavy metals besides iron (Fe), were suggested prevent graminaceous plants from cadmium (Cd) toxicity. To assess interactions between Cd and phytosiderophore-mediated Fe acquisition, maize (Zea mays) grown hydroponically under limiting supply. Exposure decreased uptake rates 59Fe(III)-phytosiderophores enhanced expression Fe-phytosiderophore transporter gene ZmYS1 in roots as well release phytosiderophore 2'-deoxymugineic acid (DMA)...
An atmospheric pressure microplasma ionization source based on a dielectric barrier discharge with helium plasma cone outside the electrode region has been developed for liquid chromatography/mass spectrometry (LC/MS). For this purpose, was realized in commercial source. Dielectric (DBDI) compared to conventional electrospray (ESI), chemical (APCI), and photoionization (APPI) positive mode. Therefore, heterogeneous compound library investigated that covered polar compounds such as amino...
Metabolic engineering of microbial cell factories for the production heterologous secondary metabolites implicitly relies on intensification intracellular flux directed toward product choice. Apart from reactions following peripheral pathways, enzymes central carbon metabolism are usually targeted enhancement precursor supply. In Pseudomonas putida, a Gram-negative soil bacterium, metabolism, i.e., required synthesis all 12 biomass precursors, was shown to be regulated at metabolic level and...
Epigenetic modifications (e.g. DNA methylation) in NAFLD and their contribution to disease progression extrahepatic complications are poorly explored. Here, we use an integrated epigenome transcriptome analysis of mouse hepatocytes identify alterations glyoxylate metabolism, a pathway relevant kidney damage via oxalate release-a harmful waste product stone-promoting factor. Downregulation hypermethylation alanine-glyoxylate aminotransferase (Agxt), which detoxifies glyoxylate, preventing...
The parallel accumulation–serial fragmentation (PASEF) approach based on trapped ion mobility spectrometry (TIMS) enables mobility-resolved and a higher number of fragments in the same time period compared to conventional MS/MS experiments. Furthermore, dimension offers novel approaches for fragmentation. Using reaction monitoring (prm), allows more accurate selection precursor windows, while using data-independent aquisition (dia) spectral quality is improved through ion-mobility filtering....
Abstract The components of secondary organic aerosols (SOAs) generated from the gas‐phase ozonolysis two C 10 H 16 ‐terpenes ( α ‐pinene; sabinene) and a cyclic 6 alkene (cyclohexene) were characterized by use Fourier transform ion cyclotron mass spectrometer equipped with an electrospray ionization source operated in negative mode. Reversed‐phase high‐performance liquid chromatography was used to achieve chromatographic separation highly oxidized compounds. In addition well‐known group low...
The labelling of two different proteins (bovine serum albumin, hen egg white lysozyme) with the commercially available chelating compound 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (p-SCN-Bn-DOTA) has been investigated. assay described here optimised for application to detect labelled by stable isotopes Eu, Tb and Ho separated sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Detection performed laser ablation ICP-MS after electroblotting target onto NC...
In this work, the combined use of desorption by a continuous wave near-infrared diode laser and ionization dielectric barrier discharge-based probe (laser discharge mass spectrometry (LD-DBDI-MS)) is presented as an ambient method for spectrometric detection nonvolatile chemicals on surfaces. A separation processes could be verified. The motivated its low cost, ease use, small size. To achieve efficient desorption, glass substrates are coated at back side with black point (target point,...
Bitterness and pungency are important parameters for olive oil quality. Therefore, two instrumental methods evaluation of these taste attributes were developed. The first one is based on the photometric measurement total phenolic compounds content, whereas second semiquantitative hydrophilic by high-performance liquid chromatography-mass spectrometry (HPLC-MS). Evaluation content was performed a modified method determination K(225) value using more specific detection pH dependency absorbance...
A Dielectric Barrier Discharge Ionization (DBDI) LC/MS interface is based on the use of a low-temperature helium plasma, which features possibility simultaneous ionization species with wide variety physicochemical properties. In this work, performance LC/DBDI-MS for trace analysis highly relevant in food and environment has been examined. Over 75 including multiclass priority organic contaminants residues such as pesticides, polycyclic aromatic hydrocarbons, organochlorine species,...
Anterograde transport of late endosomes or lysosomes (LE/Lys) is crucial for proper axon growth. However, the role energetic nutrients has been poorly explored. Malonyl-CoA a precursor fatty acids, and its intracellular levels highly fluctuate depending on glucose availability energy sensor AMP-activated protein kinase (AMPK). We demonstrate in HeLa cells that carnitine palmitoyltransferase 1C (CPT1C) senses malonyl-CoA enhances LE/Lys anterograde by interacting with endoplasmic reticulum...
While rhamnolipids of the Pseudomonas aeruginosa type are commercially available, natural diversity and their origin have barely been investigated. Here, we collected known identified new rhlA genes encoding acyltransferase responsible for synthesis lipophilic rhamnolipid precursor 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA). Generally, all homologs were found in Betaproteobacteria Gammaproteobacteria A likely horizontal gene transfer event into Actinobacteria is only exception. The...
Few human data on exposure and toxicity are available neonicotinoids neonicotinoid-like compounds (NNIs), an important group of insecticides worldwide. Specifically, assessment humans by biomonitoring remains a challenge due to the lack appropriate biomarkers. We investigated metabolism metabolite excretion in urine acetamiprid (ACE), clothianidin (CLO), flupyradifurone (FLUP), imidacloprid (IMI), sulfoxaflor (SULF), thiacloprid (THIAC) thiamethoxam (THIAM) after single oral dosages at...
Abstract Homosalate (HMS) is a UV filter used in sunscreens and personal care products as mixture of cis - trans -isomers. Systemic absorption after sunscreen use has been demonstrated humans, concerns have raised about possible endocrine activity HMS, making general population exposure assessment desirable. In previous study, it was shown that the oral bioavailability -HMS ( c HMS) lower than t by factor 10, calling for separate evaluation both isomers risk assessment. The aim current study...
Lipidomics studies require rapid separations with accurate and reliable quantification results to further elucidate the role of lipids in biological processes their functions. Supercritical fluid chromatography (SFC), particular, can provide this high-resolution separation. The combination trapped ion mobility spectrometry (TIMS) has not yet been applied, although post-ionization separation method liquid or imaging techniques already proven itself resolving isomeric isobaric preventing false...