Abstract Effective transfection of genetic molecules such as DNA usually relies on vectors that can reversibly uptake and release these molecules, protect them from digestion by nuclease. Non-viral meeting requirements are rare due to the lack specific interactions with DNA. Here, we design a series four isoreticular metal-organic frameworks (Ni-IRMOF-74-II -V) progressively tuned pore size 2.2 4.2 nm precisely include single-stranded (ssDNA, 11–53 nt), achieve reversible interaction between...

Receptor-interacting protein kinase 3 (RIP3) is a member of the RIP Ser/Thr family, plays an important role in regulating cell survival, apoptosis and necrosis. However, RIP3 carcinogenesis colorectal cancer still poorly understood.We used quantitative PCR Western blot analysis to examine expression primary paired normal mucosa. clinicopathological significance was assessed by immunohistochemical staining 112 cases with noncancerous tissues. The biological function overexpression measured...

Background Prostate cancer (PCa) is one of the prevalent malignant tumors among men. It can progress to castration-resistant prostate (CRPC), which significantly more challenging treat. Saikosaponin A (SSA), a triterpenoid saponin extracted from genus Bupleurum, exerts numerous pharmacological effects, including anti-inflammatory and anti-tumorigenic effects. However, mechanism underlying effects SSA in treatment remains elusive. Methods In this study, network pharmacology approach was...

Abstract The development of RNA aptamers/fluorophores system is highly desirable for understanding the dynamic molecular biology RNAs in vivo. Peppers-based imaging systems have been reported and applied mRNA living cells. However, need to insert corresponding aptamer sequences into target relatively low fluorescence signal limit its application endogenous imaging. Herein, we remolded original Pepper developed a tandem array inert (iPepper) turn-on system. iPepper allows efficient selective...

We report the use of metal–organic frameworks (MOFs) for selective separation nucleic acids (DNA and RNA) with different secondary structures through size, shape, length, capability conformational transition. Three MOFs precisely controlled pore environments, Co-IRMOF-74-II, -III, -IV, composed Co2+ organic linkers (II, III, IV), respectively, were used inclusion acid into their pores from solution. This was proven to be a spontaneous process disordered free state restricted ordered via...

Objective: Pirarubicin (THP), one of the anthracycline anticancer drugs, is widely used in treatment various cancers, but its cardiotoxicity cannot be ignored. Schisandrin B (SchB) has ability to upregulate cellular antioxidant defense mechanism and promote mitochondrial function status. However, it not been reported whether can resist THP-induced cardiotoxicity. The aim this study was investigate effect SchB on THP mechanism. Methods: rat model induced by established, performed at same...

Clotting factor XII (Hageman factor) contains epidermal growth (EGF)-homologous domains and is reported to be a potent mitogen for human hepatoma (HepG2) cells. In this study, we tested whether exhibits activity on several other EGF-sensitive target cells, including fetal hepatocytes, endothelial alveolar type II aortic smooth muscle We found that significantly enhanced [3H]thymidine incorporation in cells (SMCs) all tested. Tyrphostin, receptor/tyrosine kinase antagonist, inhibited both...

Pirarubicin (THP) is one of anthracycline anticancer drugs. It widely used in the treatment various cancers, but its hepatotoxicity cannot be ignored. Schisandrin B (SchB) a traditional liver-protecting drug, which has ability to promote mitochondrial function and upregulate cellular antioxidant defense mechanism. However, whether it can resist THP-induced not been reported. The purpose this study was observe explore effect SchB on potential mechanism by adding diet rats with...

The development of RNA imaging strategies in live cells is essential to improve our understanding their role various cellular functions. We report an efficient method based on the CRISPR-dPspCas13b system with fluorescent aptamers sgRNA (CasFAS) cells. Using modified attached that showed reduced background fluorescence, this approach provides a simple, sensitive way image and track endogenous high accuracy efficiency. In addition, color switching can be easily achieved by changing...

Pirarubicin (THP) is one of the most commonly used antineoplastic drugs in clinical practice. However, its application limited due to toxic and heart‑related side effects. It has been reported that oxidative stress, inflammation apoptosis are closely associated with cardiotoxicity caused by pirarubicin (CTP). Additionally, it also scutellarein (Sc) exerts anti‑inflammatory, antioxidant, cardio‑cerebral vascular protective anti‑apoptotic properties. Therefore, present study aimed investigate...

Cervical squamous cell carcinoma (CESC) is a significant threat to women’s health. Resistance cisplatin (DDP), common treatment, hinders the therapeutic efficacy. Understanding molecular basis of DDP resistance in CESC imperative. Cyclin-dependent kinase inhibitor 2A (CDKN2A) expression was evaluated through quantitative real-time-PCR and western blot clinical samples from 30 patients human cervical epithelial cells lines (SiHa, C33A, Caski). It also bioinformatics analysis Timer, Ualcan,...

Abstract Background Papillary thyroid carcinoma (PTC) is the most common type of cancer. Despite favorable prognosis in some patients, there remains a risk lymph node metastasis and death patients. Therefore, new therapeutic strategies are required to improve PTC outcomes. Methods In this study, we performed differential expression analysis using data from patients with collected Cancer Genome Atlas program database, prognostic genes. To understand effects ubiquitin-conjugating enzyme 9...

The RNA N6-methyladenosine (m6A) modification is a critical regulator of various biological processes, but precise and dynamic control m6A remains challenge. In this work, we present red/far-red light-inducible editing system that enables efficient reversible modulation levels with minimal off-target effects. By engineering the CRISPR dCas13 protein sgRNA two pairs heterodimerizing proteins, ΔphyA/FHY1 Bphp1/PspR2, achieved targeted recruitment effectors. This significantly enhances writing...

Abstract Background & Aims Previous studies have indicated that telomere length is associated with altered risk of various tumours including hepatitis B virus ( HBV )‐related hepatocellular carcinoma. However, the association between and cirrhosis has not been reported. Methods In this nested case–control study, we used real‐time quantitative PCR to determine relative RTL ) in serum DNA samples from 100 ‐related cases frequency‐matched controls, evaluated associations by logistic...

A specificity fluorescence platform for probing of sequence-specific miRNA using fluorophore labeled DNA and metal-organic frameworks (MOFs) is developed. Ni-IRMOF-74-II can quench the four fluorescent dyes in single strand oligodeoxynucleotides (ssODNs), recovers presence their target be detected corresponding channels. Furthermore, recovered intensity has a linear relationship with concentration range from 1.25 to 100 nM at This sensor detect several kinds same system time high negligible...

Pirarubicin (THP), one of the anthracycline anticancer drugs, is widely used in treatment various types cancer, but its cardiotoxicity cannot be ignored. Canagliflozin, first sodium‑glucose co‑transporter‑2 inhibitor approved by USA FDA, has been shown to have a significant effect on cardiovascular damage caused diabetes. However, it not reported whether can resist THP‑induced cardiotoxicity. The aim present study was investigate canagliflozin and mechanism. A rat model induced THP...

We reported on an efficient RNA imaging strategy based a CRISPR-Cas and Tat peptide with fluorescent aptamer (TRAP-tag). Using modified hairpin binding proteins fused array that recruits aptamers, this simple sensitive is capable of visualizing endogenous in cells high precision efficiency. In addition, the modular design CRISPR-TRAP-tag facilitates substitution sgRNAs, proteins, aptamers order to optimize quality live cell affinity. With CRISPR-TRAP-tag, exogenous GCN4, mRNA MUC4, lncRNA...

Kaempferide (Ka), a major natural active component of Tagetes erecta L, has numerous pharmacological effects such as anti-obesity, anticancer, and anti-hypertension. However, there is no clear evidence that Ka directly related to inflammation oxidative stress in obese mice. We aimed explore the on its mechanism.The mice were induced by high-fat diet (HFD). The anti-obesity effect was tested liver body weight, adiposity index, white adipose tissue. Blood sample analysis used detect...