A5027911592- Ion channel regulation and function
- Nitric Oxide and Endothelin Effects
- Cardiac electrophysiology and arrhythmias
- Ion Channels and Receptors
- Pregnancy and preeclampsia studies
- Neuroscience and Neuropharmacology Research
- Sphingolipid Metabolism and Signaling
- Hormonal Regulation and Hypertension
- Renin-Angiotensin System Studies
- Antioxidant Activity and Oxidative Stress
- Eicosanoids and Hypertension Pharmacology
- Receptor Mechanisms and Signaling
- Cardiac Ischemia and Reperfusion
- Stress Responses and Cortisol
- Lipid Membrane Structure and Behavior
- Angiogenesis and VEGF in Cancer
- Complement system in diseases
- Cellular transport and secretion
- Lysosomal Storage Disorders Research
- Adenosine and Purinergic Signaling
- Free Radicals and Antioxidants
- Thermoregulation and physiological responses
- Neuroscience and Neural Engineering
- Neurobiology and Insect Physiology Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
Ewha Womans University
2010-2023
Ewha Womans University Medical Center
2004-2023
KU Leuven
1999-2002
Dongguk University
1992
Seoul National University
1992
Nagoya City University
1992
We have compared activation by heat of TRPV4 channels, heterogeneously expressed in HEK293 cells, and endogenous channels mouse aorta endothelium (MAEC). Increasing the temperature above 25 degrees C activated currents increased [Ca(2+)](i) cells transfected with MAEC. When selective ligand 4alphaPDD (alpha-phorbol 12,13-didecanoate), heat-activated both systems showed typical biophysical properties through TRPV4, including their single channel conductance. Deletion three N-terminal ankyrin...
Excessive endothelial globotriaosylceramide (Gb3) accumulation is associated with dysfunction and impaired endothelium-dependent relaxation in Fabry disease. In cells, K(Ca)3.1 channels contribute to relaxation. However, the effect of Gb3 on underlying mechanisms Gb3-induced are unknown. Herein, we hypothesized that induces channel aimed clarify mechanisms.The animal model disease, α-galactosidase A (Gla) knockout mice, displayed age-dependent dysfunction. current expression were...
Globotriaosylceramide (Gb3) induces KCa3.1 downregulation in Fabry disease (FD). We investigated whether Gb3 endocytosis and degradation.KCa3.1, especially plasma membrane-localized KCa3.1, was downregulated both Gb3-treated mouse aortic endothelial cells (MAECs) human umbilical vein cells. Gb3-induced prevented by lysosomal inhibitors but not a proteosomal inhibitor. Endoplasmic reticulum stress-inducing agents did induce downregulation. upregulated the protein levels of early endosome...
To determine whether lysophosphatidylcholine (LPC) induces endothelial cell injury by altering the production of nitric oxide (NO) and thereby increasing reactive oxygen species (ROS).Human umbilical vein cells (HUVECs) were cultured exposed to LPC, LPC with N(G)-nitro-l-arginine methyl ester (L-NAME), antioxidants. LPC-induced viability determined using LDH Resazurin assays. The Mann-Whitney U test was used for statistical analysis.LPC induced HUVEC in a concentration-dependent manner....
Abstract Objectives The aim of this study was to explore the effect essential oil Citrus bergamia Risso (bergamot) on mouse blood vessels and analyse mechanism from a pharmacological perspective. Methods We investigated bergamot (BEO) vascular tonus during contraction aorta induced by prostaglandin F2α (PGF2α) or noradrenaline (norepinephrine). Key findings In aortic rings, BEO (0.01, 0.1 0.2% v/v) reduced in dose-dependent manner, relaxed PGF2α. No significant difference extent...
Circulating CTRP1 (C1q/TNF-α [tumor necrosis factor-α]-related protein 1) levels are increased in hypertensive patients compared with those healthy subjects. Nonetheless, little is known about the molecular and physiological function of blood pressure (BP) regulation.To investigate physiological/pathophysiological role BP regulation.CTRP1 production was to maintain normotension under dehydration conditions, this impaired inducible KO (knockout) mice (CTRP1 ΔCAG). The increase conditions...
K(Ca) 1.1 regulates smooth muscle contractility by modulating membrane potential, and age-associated changes in expression may contribute to the development of motility disorders gastrointestinal tract. Sphingolipids (SLs) are important structural components cellular membranes whose altered composition affect expression. Thus, this study, we examined whether SL due aging gastric (GSM). We studied ceramide synthases (CerS) levels GSM mice varying ages compared them with those young CerS2-null...
Endothelial oxidative stress develops with aging and reactive oxygen species impair endothelium-dependent relaxation (EDR) by decreasing nitric oxide (NO) availability. KCa3.1, which contributes to EDR, is upregulated H2O2. We investigated whether KCa3.1 upregulation compensates for diminished EDR NO during aging-related stress. Previous studies identified that the levels of ceramide synthase 5 (CerS5), sphingosine, sphingosine 1-phosphate were increased in aged wild-type CerS2 mice. In...
The effect of sphingosine-1-phosphate (S1P) on large-conductance Ca 2+ -activated K + (BK ) channels was examined in primary cultured human umbilical vein endothelial cells by measuring intracellular concentration ([Ca ] i ), whole cell membrane currents, and single-channel activity. In nystatin-perforated current-clamped cells, S1P hyperpolarized the simultaneously increased [Ca . potentials were strongly correlated. clamped BK currents activated increasing via dialysis with pipette...
Resistin-like molecule alpha (Retnla), also known as 'Found in inflammatory zone 1', is a secreted protein that has been found bronchoalveolar lavage (BAL) fluid of ovalbumin (OVA)-induced asthmatic mice and plays role regulator T helper (Th)2-driven inflammation. However, the Retnla progress Th2-driven airway inflammation not yet clear. To better understand function inflammation, we generated Retnla-overexpressing (Retnla-Tg) mice. Retnla-Tg showed increased expression BAL epithelial cells....