A5005769254- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Cardiac Fibrosis and Remodeling
- Cerebrovascular and Carotid Artery Diseases
- Coronary Interventions and Diagnostics
- MicroRNA in disease regulation
- Cancer Treatment and Pharmacology
- Cardiac Ischemia and Reperfusion
- Kruppel-like factors research
- Cancer-related molecular mechanisms research
- Cardiac Structural Anomalies and Repair
- Antiplatelet Therapy and Cardiovascular Diseases
- Atherosclerosis and Cardiovascular Diseases
- Circular RNAs in diseases
- Cancer-related gene regulation
- Cardiovascular Health and Disease Prevention
- Extracellular vesicles in disease
- Pesticide Exposure and Toxicity
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Fibroblast Growth Factor Research
- Neurological Disease Mechanisms and Treatments
- Congenital heart defects research
- Cardiac electrophysiology and arrhythmias
Chungbuk National University
2002-2023
Asan Medical Center
1998-2023
Seoul National University Bundang Hospital
2023
Temple University
2019-2021
Inha University
2019
Korea National Institute of Health
2014-2019
Sungkyunkwan University
2016
Ajou University
2016
Samsung Medical Center
2016
Yonsei University
2016
Despite development of therapeutic modalities, myocardial ischemia-reperfusion (I/R) injury remains an important cause cardiac dysfunction. Multiple strategies exist experimentally, but few are clinically available. Nuclear factor kappa-B (NF-κB) is a key transcription in the inflammatory response and implicated I/R injury. We hypothesized that NFκB inhibitor BAY 11-7082 (BAY) would decrease extent after I/R. Hypoxia-reoxygenation (H/R) was induced rat neonatal cardiomyocytes with or without...
Oxytocin stimulates the cardiomyogenesis of embryonic stem cells and adult cardiac cells. We previously reported that oxytocin has a promigratory effect on umbilical cord blood-derived mesenchymal (UCB-MSCs). In this study, UCB-MSCs were cultured with examined for their therapeutic in an infarcted heart. pretreated 100 n<i>M</i> markers assessed by immunofluorescence staining. Next, oxytocin-supplemented USC-MSCs (OT-USCs) cocultured hypoxia/reoxygenated neonatal rat...
Abstract We examined whether a shift in macrophage phenotype could be therapeutic for myocardial infarction ( MI ). The mouse cell line RAW 264.7 was stimulated with peptidoglycan PGN ), or without 5‐azacytidine (5 AZ ) treatment. induced by ligation of the left anterior descending coronary artery rats, and rats were divided into two groups; saline‐injection group 5 ‐injection (2.5 mg/kg/day, intraperitoneal injection). LV function evaluated immunohistochemical analyses performed 2 weeks...
In this study, we established and characterized human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) from four different donors. However, the hUCB-MSCs showed remarkable variations in their therapeutic efficacy for repairing rat infarcted myocardium (including process of angiogenesis) 8 weeks after transplantation. addition, observed that level vascular endothelial growth factor (VEGF) is correlated with hUCB-MSCs. Next, to investigate practical application hUCB-MSCs,...
Differentiation and de-differentiation of vascular smooth muscle cells (VSMCs) are important events in atherosclerosis restenosis after angioplasty. MicroRNAs considered a key regulator cellular processes such as differentiation, proliferation, apoptosis. Here, we report the role new miR-18a-5p microRNA its downstream target genes VSMCs carotid balloon injury model.Expression candidate was examined artery model by quantitative reverse-transcription polymerase chain reaction (qRT-PCR)...
Optimization of the specific affinity cardiac delivery vector could significantly improve efficiency gene/protein delivery, yet no vectors to date have sufficient target specificity for myocardial infarction (MI). In this study, we explored bacterial tropism infarcted myocardium based on our previous observations that certain bacteria are capable targeting hypoxic regions in solid tumors. Out several Escherichia coli or Salmonella typhimurium strains, S. defective synthesis ppGpp (ΔppGpp...
Abstract Background Angiogenesis is the main therapeutic mechanism of cell therapy for cardiovascular diseases, but diabetes reported to reduce function and number progenitor cells. Therefore, we studied effect streptozotocin-induced on bone marrow-mesenchymal stem (MSC) function, examined whether diabetes-impaired MSC could be rescued by pretreatment with oxytocin. Results MSCs were isolated cultured from diabetic (DM) or non-diabetic (non-DM) rat, proliferation rate was compared. DM-MSC...
Vascular smooth muscle cells (VSMCs) modulate their phenotype between synthetic and contractile states in response to environmental changes; this modulation plays a crucial role the pathogenesis of restenosis atherosclerosis. Here, we identified fibroblast growth factor 12 (FGF12) as novel key regulator VSMC switch.Using murine models human specimens, found that FGF12 was highly expressed VSMCs normal vessel walls but downregulated from injured atherosclerotic vessels. In VSMCs, expression...
To develop the functionalized superparamagnetic iron oxide nanoparticles (SPIONs) demonstrating capacities to be delivered in antigen presenting cells specifically and dispersed physiological environment stably, nanoparticle surface was coated with mannan that induces receptor-mediated endocytosis. Mannan is a water-soluble polysaccharide having high content of D-mannose residues recognized by mannose receptors on immunate macrophages. Mannan-coated SPIONs (mannan-SPIONs) were prepared...
Abstract Recent studies show that oxytocin has various effects on cellular behaviors. Oxytocin is reported to stimulate cardiomyogenesis of embryonic stem cells and endothelial cell proliferation. Mesenchymal (MSCs) are widely used for cardiac repair, we elucidated the effect umbilical cord derived‐MSCs (UCB‐MSCs). UCB‐MSCs were pretreated with (100 nM) washed saline prior experiments. To evaluate their angiogenic potential migration activity, tube formation assay Boyden chamber performed....
Vascular smooth muscle cell (VSMC) proliferation plays a key role in neointimal hyperplasia and restenosis. Here we report the of microRNA miR‐142‐5p its downstream target genes on cultured VSMCs. promoted VSMC by down‐regulating B translocation gene 3 (BTG3). We found that BTG3 inhibited expression cycle regulatory growth. As shown luciferase reporter assay, bound directly to 3′‐untranslated region BTG3. Overexpression induced genes. Thus, BTG3, novel, direct miR‐142‐5p, negatively...
The female reproductive system becomes fertile through the action of hormones involved in hypothalamic-pituitary-ovarian axis. On other hand, estrogen-like endocrine disruptors released into environment come contact with humans by various routes and affect system. Exposure to these chemicals can cause problems process, from egg ovulation implantation, or diseases. These infertility. Decamethylcyclopentasiloxane (D5) is used for lubrication silicone polymers, households, personal care...
Paclitaxel has been shown to inhibit vascular smooth muscle cell migration and proliferation contributing neointimal formation. This study tested whether novel oral formulations of paclitaxel can prevent formation in a rat carotid artery injury model.Oral (0, 5, 7.5, or 10 mg/kg) were administered 40 rats by gavage for 5 days after injury. The peak plasma levels at mg/kg 61+/-16, 89+/-22, 108+/-28 nmol/L, respectively. Treatment effects assessed 11 angiographic minimum luminal diameters the...