Genes from Bacillus subtilis predicted to encode a phosphate-specific transport (Pst) system were shown by mutation affect high-affinity Pi uptake but not arsenate resistance or phosphate (Pho) regulation. The transcription start of the promoter upstream pstS gene was defined primer extension. contains structural features analogous Escherichia coli pst sequence similarity. Expression this induced >5,000-fold upon starvation and regulated PhoP-PhoR two-component regulatory system. These...

The Bacillus subtilis pstS operon and phoA gene are members of the Pho regulon that is controlled by PhoR, a histidine kinase, PhoP, response regulator. Footprinting analysis showed phosphorylated PhoP extended protected region in promoters, also bound to separate site within coding each gene. Our previous vivo studies have shown that, contrast other promoters not expressed either phoP or phoR mutants, low‐level induction from promoter (25% parent strain) can be detected mutant. In this...

Abstract The pathogenesis of multiple myeloma (MM) is thought to involve at least two pathways, which generate hyperdiploid (HRD) or nonhyperdiploid (NHRD) tumors, respectively. Apart from chromosome content, the pathways are distinguished by five primary immunoglobulin heavy chain ( IGH ) rearrangements (4p16, FGFR3 , and MMSET ; 6p21, CCND3 11q13, CCND1 16q23, MAF 20q12, MAFB that present mainly in NHRD tumors. To determine prevalence structures IG light chain, MYC genomic MM, we have done...

To complete the data of ocular trauma in central China, as a well-known tertiary referral center for trauma, we documented epidemiological characteristics and visual outcomes patients hospitalized this region.A retrospective study China from 2006 to 2011 was performed.This included 5964 eyes 5799 patients. The average age 35.5±21.8y with male-to-female ratio 2.8:1. most common 45-59y group. Most were farmers workers (51.9%). injuries firework related (24.5%), road traffic (24.2%), work...

SUV39H2 (suppressor of variegation 3-9 homolog 2), which introduces H3K9me3 to induce transcriptional repression, has been reported play critical roles in heterochromatin maintenance, DNA repair, and recently, carcinogenesis. Dysregulation expression observed several types cancers. However, neither the genomic landscape nor clinical significance lung adenocarcinoma probed comprehensively. In this research, we conducted bioinformatics analysis primarily sort out potential genes with...

Bacillus subtilis PhoP and PhoR, a pair of two‐component regulatory proteins, regulate the phosphate starvation response. Here, we used two other pho regulon promoters, phoA pstS to examine mechanism PhoP‐specific activation its target promoters. Both gel shift DNase I footprinting assays indicate that bound Unphosphorylated only multiple TTAACA‐like sequences upstream these while phosphorylated extended binding region in both 5′ 3′ direction and, additionally, protected internal coding...

ABSTRACT tagA , tagD and tuaA operons are responsible for the synthesis of cell wall anionic polymer, teichoic acid, teichuronic respectively, in Bacillus subtilis . Under phosphate starvation conditions, acid is synthesized while inhibited. Expression these genes controlled by PhoP-PhoR, a two-component system. It has been proposed that PhoP∼P plays key role activation repression In this study, we demonstrated switch process from to synthesis, using an vitro transcription The results...

Human uveal melanoma (UM) is a common ocular malignant tumor with high risk of metastasis. Emerging evidence indicates that long non-coding RNAs (lncRNAs) are correlated the development UM. Here, we aimed to determine biological significance lncRNA growth arrest-specific transcript 5 (GAS5) in UM.The expression levels GAS5 and microRNA-21 (miR-21) UM tissues cells were detected by qRT-PCR analysis. CCK-8 assay was performed investigate viability after cell transfections, migration invasion...

Purpose . To investigate the prognostic factors for visual outcome in traumatic cataract patients. Methods The demographic features of patients Central China were studied. that might influence analyzed. sensitivity and specificity OTS (ocular trauma score) predicting VA calculated. Results study enrolled 480 cases. 65.5% achieved at >20/60. associated with final initial VA, injury type, wound location, way removal, IOL implantation. sensitivities NLP (nonlight perception), LP/HM (light...

The accurate role of ANRIL in cataract is poorly understood. We aimed to reveal the effects on H2O2-treated HLECs, SRA01/04, as well regulatory mechanisms. Oxidative stress model HLECs was induced by H2O2. Cell injury evaluated according cell proliferation, apoptosis and DNA damage using CCK-8 assay/flow cytometry TUNEL assays/γH2AX staining. Expressions miR-21 were determined RT-qPCR. miR-21, miR-34a miR-122-5p inhibition AMPK β-catenin with overexpression H2O2 stimulation analyzed. In vivo...

Alkaline phosphatase (APase) is induced as a culture enters stationary phase because of limiting phosphate. The results presented here show that expression APase regulated both negatively and positively. PhoP, homolog family bacterial transcription factors, PhoR, histidine protein kinases, are required for induction APases when phosphate becomes limiting. period lasts 2 to 3 h, after which the rate accumulation decreased. Mutant strains defective in Spo0A factor failed decrease production....

AIM: To study the potential role of fasudil as a treatment for posterior capsular opacification (PCO) human crystalline lens. METHODS: Human lens epithelial cells (HLECs; line SRA01/04) was exposed to transforming growth factor-β2 (TGF-β2) induce process epithelial-mesenchymal transition (EMT). Fasudil applied cell samples. Its effect on overall HLECs proliferation and migration studied, its influence EMT induction by TGF-β2 using assay, MTT colorimetric assay Western blot assay. RESULTS:...

The aim of this study was to report the incidence and management ocular firecracker trauma assess predictive value an Ocular Trauma Score (OTS) in trauma. includes consecutive case retrospective analysis Numerical values rendered OTS variables (visual acuity, rupture, endophthalmitis, perforating or penetrating injury, retinal detachment, afferent pupillary defect) at presentation were summated converted categories. likelihood final visual acuities each category calculated. sensitivity...

Although lots of long non-coding RNAs (lncRNAs) have been demonstrated to be involved in carcinogenesis, the functions numerous lncRNAs remain unknown. Bioinformatics online database showed that lncRNA LOC100132707 was highly expressed metastatic melanoma tissues, and its expression predicted a lower overall survival rate patients. However, function uveal (UM) progression still remains unclear. In present study, we aimed elucidate role molecular mechanisms underlying UM.RT-PCR used detect...

For an ISP (Internet Service Provider) which has deployed P2P caches in more than one AS (autonomous system), cooperative caching makes their cooperate with each other can save cost of carrying traffic independent for the ISP. However, existing schemes only use objects' popularity as measurement to decide objects should be cached, and on intra-ISP links that great impact benefits is not considered. In this paper, we propose a novel scheme named C-LGV (Cooperative, Lowest Global Value). uses...

Objective To evaluate the clinical efficiency of application iris retractors for treatment subluxation lens in patients with Marfan’s syndrome. Methods This study comprised 20 eyes 12 syndrome and lens. The ranges were all more than 180 degrees. After continuous curvilinear capsulorrhexis, 2-3 used to drag fixate edge capsulorhexis opening. Following phacoemulsification aspiration, residual capsular bag was removed. Anterior vitreous resection performed if prolapsed after removal bag....

Objective To explore the clinical characteristics of electric bicycle related eye injury.Methods Consecutive selection and retrospective analysis injuries 62 cases (71 eyes) to investigate its characteristics.Results The ratio male female was 3.4.The ages were 17 years old,at average (35.6 ±6.8) old.9 bilateral injuries,accounting for 14.5％.Worker peasant main parts patients.11.3％ patients only had ocular 88.7％ combined with other outside eyes.59.2％ eyes open global injury.42.3％ underwent...

Objective To measure the prognostic value of ocular trauma score (OTS) in open globe injuries.Methods Consecutive selection injuries admitted to our hospital recent 2 years.The clinical manifestations were retrospectively analyzed.The patient' s OTS was calculated and compared with visual acuity at last follow-up evaluate ability injuries.Results 88 eyes cases enrolled study.Male patients accounted for 69.32％.The most common injury area Ⅰ (63.64％).The mainly 45 ～ 65 sector.The sensitivity...

Background Epithelial-mesenchymal transition (EMT) is a major event in the pathogenesis of posterior capsular opacification (PCO),and expressions α-smooth muscle actin (α-SMA) marker EMT.Previous studies showed that breviscapine plays an important role anti-fibrosis and suppression EMT,however,the mechanism its effect on EMT LECs unclear.Objective Present study was to investigate expression α-SMA fibronectin (FN) human induced by transforming growth factor-β2 (TGF-β2).Methods Human...