A5083441262- Neurobiology and Insect Physiology Research
- Mosquito-borne diseases and control
- Physiological and biochemical adaptations
- Insect Resistance and Genetics
- Insect Pest Control Strategies
- Insect and Pesticide Research
- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Invertebrate Immune Response Mechanisms
- Aquaculture Nutrition and Growth
- Connexins and lens biology
- Insect and Arachnid Ecology and Behavior
- Insect symbiosis and bacterial influences
- Plant Stress Responses and Tolerance
- Insect Utilization and Effects
- Insect-Plant Interactions and Control
- Essential Oils and Antimicrobial Activity
- Fish Ecology and Management Studies
- Research on Leishmaniasis Studies
- Malaria Research and Control
- Trypanosoma species research and implications
- Viral Infectious Diseases and Gene Expression in Insects
- Bacillus and Francisella bacterial research
- Traditional and Medicinal Uses of Annonaceae
- Crustacean biology and ecology
The Ohio State University
2016-2025
J. Craig Venter Institute
2018
College of Wooster
2013-2015
Cornell University
2008-2011
New York State College of Veterinary Medicine
2009
Yale University
2005-2007
University of Florida
1998-2002
Mount Desert Island Biological Laboratory
1999-2001
The gill of fishes is modified for gas exchange, thereby providing a site net movement salts and water down their respective gradients. Specialized cells in the epithelium are joined by tight junctions variable depth express variety transporters channels. These mediate NaCl extrusion marine uptake freshwater fishes. transport steps also provide pathways ammonia acid vs. base equivalents. J. Exp. Zool. 283:641–652, 1999. © 1999 Wiley-Liss, Inc.
Pendrin is an anion exchanger in the cortical collecting duct of mammalian nephron that appears to mediate apical Cl − /HCO[Formula: see text]exchange bicarbonate-secreting intercalated cells. The goals this study were determine 1) if pendrin immunoreactivity was present gills a euryhaline elasmobranch (Atlantic stingray, Dasyatis sabina), and 2) branchial influenced by environmental salinity. Immunoblots detected Atlantic stingray gills; greatest freshwater stingrays compared with...
Abstract Insecticide resistance is a growing threat to mosquito control programs around the world, thus creating need discover novel target sites and target-specific compounds for insecticide development. Emerging evidence suggests that inward rectifier potassium (Kir) channels represent viable molecular targets developing insecticides with new mechanisms of action. Here we describe discovery characterization VU041, submicromolar-affinity inhibitor Anopheles (An.) gambiae Aedes (Ae. )...
SUMMARY In the gills of freshwater teleost fishes, vacuolar proton-ATPase (V-H+-ATPase) is found on apical membrane pavement and chloride (Na+/K+-ATPase-rich) cells, an important transporter for energizing Na+ uptake H+ excretion. elasmobranch V-H+-ATPase has not been extensively studied its expression in individuals examined. The goals this study were to examine effects environmental salinity (the Atlantic stingray, Dasyatis sabina) determine if Na+/K+-ATPase are expressed same cells. We...
ABSTRACT Changes in Na+/K+-ATPase activity and abundance associated with environmental salinity were investigated the gills rectal gland of Atlantic stingray Dasyatis sabina. Using a ouabain-specific ATPase assay western blotting, we found that stingrays from fresh water had highest relative gills. immunohistochemistry, also freshwater greatest number Na+/K+-ATPase-rich cells. When acclimated to sea for 1 week, cells decreased In seawater stingrays, branchial further reduced. glands, lower...
Others report that carbonic anhydrase II (CA II) binds to the C termini of anion exchanger AE1 and electrogenic Na/HCO3 cotransporter NBCe1-A, enhancing transport. After injecting oocytes with NBCe1-A cRNA (Day 0), we measured NBC current (INBC) by two-electrode voltage clamp 3), injected CA protein + Tris or just INBC initial rate at which intracellular pH fell (dpHi/dt) upon applying 5% CO2 4), exposed permeant inhibitor ethoxzolamide (EZA), dpHi/dt 4). Because was greater in than oocytes,...
Mosquito-borne diseases such as malaria and dengue fever take a large toll on global health. The primary chemical agents used for controlling mosquitoes are insecticides that target the nervous system. However, emergence of resistance in mosquito populations is reducing efficacy available insecticides. development new therefore urgent. Here we show VU573, small-molecule inhibitor mammalian inward-rectifying potassium (Kir) channels, inhibits Kir channel cloned from renal (Malpighian) tubules...
The 50-year-old Aedes albopictus C6/36 cell line is a resource for the detection, amplification, and analysis of mosquito-borne viruses including Zika, dengue, chikungunya. derived from an unknown number larvae unspecified strain mosquitoes. Toward improved utility research in virus transmission, we present annotated assembly genome.The genome has largest contig N50 (3.3 Mbp) any mosquito assembly, presents sequences both haplotypes most diploid genome, reveals independent null mutations...
Background. In adult female mosquitoes, the renal (Malpighian) tubules play an important role in post-prandial diuresis, which removes excess ions and water from hemolymph of mosquitoes following a blood meal. After roles that Malpighian processing meals are not well described. Methods. We used combination next-generation sequencing (paired-end RNA sequencing) physiological/biochemical assays Asian tiger (Aedes albopictus) to generate molecular functional insights into how they may...
The goals of this study were to (1) measure plasma osmolytes and rectal gland weights a freshwater (FW) Atlantic stingray (Dasyatis sabina) population in the St. Johns River, Florida, (2) determine how these parameters change after acclimation seawater (SW). We hypothesized that FW D. sabina may show physiological divergence from marine sabina, because individuals reproduce complete their life cycle River. hyperregulate osmolality (621.4 mOsm kg−1), with Na+, Cl−, urea concentrations 211.9,...
Based on solid-phase binding assays with enzyme-linked immunosorbent assay detection, previous investigators suggested that intracellular carbonic anhydrase II (CA II) interacts at high affinity the C-terminal (Ct) domains of SLC4 bicarbonate-transport proteins, expressed as glutathione S-transferase (GST) fusion to form functional HCO3- metabolons. Here we re-evaluated this protein-protein interaction using two assays. We first compared ability Ct domain three transporters, SLC4-A1 (AE1),...
SUMMARY We compared levels of the major organic osmolytes in muscle elasmobranchs, including methylamines trimethylamine oxide (TMAO), betaine and sarcosine as well β-amino acids taurine β-alanine,and activities enzymes methylamine synthesis (betaine TMAO) species with a wide range urea contents. Four marine, euryhaline freshwater (Dasyatis sabina), two species, one that accumulates (Himantura signifer) does not(Potamotrygon motoro), were analyzed. Urea contents ranged from 229–352 μmol g–1...
Vector-borne diseases such as dengue fever and malaria, which are transmitted by infected female mosquitoes, affect nearly half of the world's population. The emergence insecticide-resistant mosquito populations is reducing effectiveness conventional insecticides threatening current vector control strategies, has created an urgent need to identify new molecular targets against novel classes can be developed. We previously demonstrated that small molecule inhibitors mammalian Kir channels...
The consumption of a vertebrate blood meal by adult female mosquitoes is necessary for their reproduction, but it also presents significant physiological challenges to mosquito osmoregulation and metabolism. renal (Malpighian) tubules play critical roles in the initial processing excreting excess water salts that are ingested. However, unclear how contribute metabolism excretion wastes (e.g., heme, ammonia) produced during digestion blood.Here we used RNA-Seq examine global changes...
Introduction - The life history of Aedes aegypti presents diverse challenges to its diuretic system. During the larval and pupal stages mosquitoes are aquatic. With emergence adult they become terrestrial. This shifts organism within minutes from an aquatic environment a terrestrial where dehydration has be avoided. In addition, female take large blood meals, which present entirely new set salt water homeostasis. Methods To determine differences in gene expression associated with these...
The goal of this study was to identify and characterize the hypothesized apical cation/H(+) exchanger responsible for K(+) and/or Na(+) secretion in renal (Malpighian) tubules yellow fever mosquito Aedes aegypti. From Malpighian tubules, we cloned "AeNHE8," a full-length cDNA encoding an ortholog mammalian Na(+)/H(+) 8 (NHE8). expression AeNHE8 transcripts is ubiquitous among tissues not enriched tubules. Western blots suggest that expressed primarily as intracellular protein, which...
Transepithelial fluid secretion across the renal (Malpighian) tubule epithelium of mosquito (Aedes aegypti) is energized by vacuolar-type (V-type) H(+)-ATPase and not Na(+)-K(+)-ATPase. Located at apical membrane principal cells, V-type translocates protons from cytoplasm to lumen. Secreted are likely derive metabolic H(2)CO(3), which raises questions about handling HCO(3)(-) cells. Accordingly, we tested hypothesis that a Cl/HCO(3) anion exchanger (AE) related solute-linked carrier 4 (SLC4)...
Mosquitoes are important disease vectors that transmit a wide variety of pathogens to humans, including those cause malaria and dengue fever. Insecticides have traditionally been deployed control populations disease-causing mosquitoes, but the emergence insecticide resistance has severely limited number active compounds used against mosquitoes. Thus, improve resistant mosquitoes there is need identify new targets for development. Recently we demonstrated inward rectifier potassium (Kir)...
Plants produce various secondary metabolites that offer a potential source of novel insecticides and repellents for the control mosquito vectors. genus Cinnamosma are endemic to, widely-distributed throughout, island Madagascar. The barks these species commonly used in traditional medicines treating wide range maladies. therapeutic nature bark is thought to be associated with its enrichment pungent drimane sesquiterpenes, which elicit antifeedant toxic effects some insects. Here we test...