A5048174967- Insect Resistance and Genetics
- Chromosomal and Genetic Variations
- Mosquito-borne diseases and control
- Malaria Research and Control
- Insect symbiosis and bacterial influences
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Invertebrate Immune Response Mechanisms
- RNA Research and Splicing
- Plant and animal studies
- Insect and Pesticide Research
- Nuclear Structure and Function
- Insect and Arachnid Ecology and Behavior
- Animal Genetics and Reproduction
- Viral Infections and Vectors
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Virus Research Studies
- Evolution and Genetic Dynamics
- Genetic diversity and population structure
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Neurobiology and Insect Physiology Research
- Microtubule and mitosis dynamics
- Insect Pest Control Strategies
Virginia Tech
2016-2025
National Research Tomsk State University
2016-2025
Life Science Institute
2010-2022
Institute of Cytology and Genetics
2011-2021
Institut thématique Génétique, génomique et bioinformatique
2015
University of Notre Dame
2002-2009
University at Buffalo, State University of New York
2001-2003
Zero to Three
2001
Introgressive hybridization is now recognized as a widespread phenomenon, but its role in evolution remains contested. Here, we use newly available reference genome assemblies to investigate phylogenetic relationships and introgression medically important group of Afrotropical mosquito sibling species. We have identified the correct species branching order resolve contentious phylogeny show that lineages leading principal vectors human malaria were among first split. Pervasive autosomal...
Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of developing tools to fight them has been slowed by lack a high-quality genome assembly. Here we combine diverse technologies produce markedly improved, fully re-annotated AaegL5 assembly, demonstrate how it accelerates mosquito science. We anchored physical cytogenetic maps, doubled number...
Sex determination in the mosquito Aedes aegypti is governed by a dominant male-determining factor (M factor) located within Y chromosome-like region called M locus. Here, we show that an M-locus gene, Nix, functions as A. aegypti. Nix exhibits persistent linkage and early embryonic expression, two characteristics required of factor. knockout with clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 resulted largely feminized genetic males production female isoforms key...
Abstract Background Genes involved in post-mating processes of multiple mating organisms are known to evolve rapidly due coevolution driven by sexual conflict among male-female interacting proteins. In the malaria mosquito Anopheles gambiae - a monandrous species which is expected be absent or minimal recent data strongly suggest that proteolytic enzymes specifically expressed female lower reproductive tissues processing male products transferred females during mating. order better...
Abstract Background Y chromosomes are responsible for the initiation of male development, fertility, and other male-related functions in diverse species. However, genes rarely characterized outside a few model species due to arduous nature studying repeat-rich Y. Results The chromosome quotient (CQ) is novel approach systematically discover genes. In CQ method, genomic DNA from males females sequenced independently aligned candidate reference sequences. female ratio number alignments...
Mosquito control remains a central pillar of efforts to reduce malaria burden in sub-Saharan Africa. However, insecticide resistance is entrenched vector populations, and countries with high face daunting challenge sustain limited set surveillance intervention tools. Here we report on the second phase project build an open resource high-quality data genome variation among natural populations major African species Anopheles gambiae coluzzii . We analyzed whole genomes 1142 individual...
Abstract Background Anopheles stephensi is the key vector of malaria throughout Indian subcontinent and Middle East an emerging model for molecular genetic studies mosquito-parasite interactions. The type form species responsible majority urban transmission across its range. Results Here, we report genome sequence annotation strain An. . 221 Mb assembly represents more than 92% entire was produced using a combination 454, Illumina, PacBio sequencing. Physical mapping assigned 62% onto...
Y chromosomes control essential male functions in many species, including sex determination and fertility. However, because of obstacles posed by repeat-rich heterochromatin, knowledge chromosome sequences is limited to a handful model organisms, constraining our understanding biology across the tree life. Here, we leverage long single-molecule sequencing determine content structure nonrecombining primary African malaria mosquito, Anopheles gambiae We find that An. consists almost entirely...
Bumblebees are a diverse group of globally important pollinators in natural ecosystems and for agricultural food production. With both eusocial solitary life-cycle phases, some social parasite species, they especially interesting models to understand evolution, behavior, ecology. Reports many species decline point pathogen transmission, habitat loss, pesticide usage, global climate change, as interconnected causes. These threats bumblebee diversity make our reliance on handful well-studied...
Chromosomes are hierarchically folded within cell nuclei into territories, domains and subdomains, but the functional importance evolutionary dynamics of these hierarchies poorly defined. Here, we comprehensively profile genome organizations five Anopheles mosquito species show how different levels chromatin architecture influence each other. Patterns observed on Hi-C maps associated with known cytological structures, epigenetic profiles, gene expression levels. Evolutionary analysis reveals...
Abstract Background Understanding genome organization and evolution is important for species involved in transmission of human diseases, such as mosquitoes. Anophelinae Culicinae subfamilies mosquitoes show striking differences sizes, sex chromosome arrangements, behavior, ability to transmit pathogens. However, the genomic basis these not fully understood. Methods In this study, we used a combination advanced technologies Oxford Nanopore Technology sequencing, Hi-C scaffolding, Bionano,...
Abstract A novel cellular response of midgut progenitors (stem cells and enteroblasts) to Plasmodium berghei infection was investigated in Anopheles stephensi . The presence developing oocysts triggers proliferation that is modulated by the Jak/STAT pathway proportional number on individual midguts. percentage parasites direct contact with enteroblasts increases over time, as proliferate. Silencing components key signaling pathways through RNA interference (RNAi) enhance progenitor...
Paracentric chromosomal inversions are major architects of organismal evolution and have been associated with adaptations relevant to malaria transmission in anopheline mosquitoes. The processes responsible for their origin maintenance, still poorly understood, can be illuminated by analysis inversion breakpoint sequences. Here, we report the structure 2La from principal vector Anopheles gambiae its relatives A. complex. distal proximal breakpoints standard (2L+a) arrangement contain gene...
Complementing genome sequence with deep transcriptome and proteome data could enable more accurate assembly annotation of newly sequenced genomes. Here, we provide a proof-of-concept an integrated approach for analysis the Anopheles stephensi, which is one most important vectors malaria parasite. To achieve broad coverage genes, carried out sequencing profiling multiple anatomically distinct sites. Based on transcriptomic alone, identified corrected 535 events incomplete involving 1196...
Abstract Background New sequencing technologies have lowered financial barriers to whole genome sequencing, but resulting assemblies are often fragmented and far from ‘finished’. Updating multi-scaffold drafts chromosome-level status can be achieved through experimental mapping or re-sequencing efforts. Avoiding the costs associated with such approaches, comparative genomic analysis of gene order conservation (synteny) predict scaffold neighbours (adjacencies) offers a potentially useful...
In tropical Africa, Anopheles funestus is one of the three most important malaria vectors. We physically mapped 157 A. complementary DNAs (cDNAs) to polytene chromosomes this species. Sequences cDNAs were in silico gambiae genome as part a comparative genomic study synteny, gene order, and sequence conservation between gambiae. These species are same subgenus diverged about recently humans chimpanzees. Despite nearly perfect preservation we found substantial shuffling order along...
Polyploid giant cells are produced as part of the response p53 mutant Burkitt's lymphoma cell lines to high doses irradiation. arise by endo‐reduplication in first week after a single 10 Gray dose Within sub‐nuclear structure is apparent and within this, autonomy evident, displayed independent nuclear DNA replication different parts nucleus. The majority these soon die apoptotic polykaryons. However, approximately 10–20% remain viable into second irradiation begin vigorous extrusion large...
An initial comparative genomic study of the malaria vector Anopheles gambiae and yellow fever mosquito Aedes aegypti revealed striking differences in genome assembly size abundance transposable elements between two species. However, chromosome arms homology An. Ae. aegypti, as well distribution genes repetitive chromosomes remained largely unexplored because lack a detailed physical map for mosquito. Using molecular landmark-guided fluorescent situ hybridization approach, we mapped 624 Mb to...
The 50-year-old Aedes albopictus C6/36 cell line is a resource for the detection, amplification, and analysis of mosquito-borne viruses including Zika, dengue, chikungunya. derived from an unknown number larvae unspecified strain mosquitoes. Toward improved utility research in virus transmission, we present annotated assembly genome.The genome has largest contig N50 (3.3 Mbp) any mosquito assembly, presents sequences both haplotypes most diploid genome, reveals independent null mutations...
Background Aedes aegypti, the yellow fever mosquito, is an efficient vector of arboviruses and a convenient model system for laboratory research. Extensive linkage mapping morphological molecular markers localized number quantitative trait loci (QTLs) related to mosquito's ability transmit various pathogens. However, linking QTLs Ae. aegypti chromosomes genomic sequences has been challenging because poor quality polytene highly fragmented genome assembly this species. Methodology/Principal...
Chromosomal inversions play a fundamental role in evolution and have been shown to regulate epidemiologically important traits malaria mosquitoes. However, they never characterized Aedes aegypti, the major vector of arboviruses, because poor structure its polytene chromosomes. In this study, we applied Hi-C proximity ligation approach identify chromosomal 25 strains Ae. acquired from worldwide distribution, as well one strain mascarensis. The study identified 21 multi-megabase with uneven...
The African malaria vector Anopheles gambiae is polymorphic for alternative arrangements on the left arm of chromosome 2 (2La and 2L+(a)) that are non-randomly distributed with respect to degree aridity. Detailed studies ecological role inversion 2La have been hindered by technical demands traditional karyotype analysis sex- stage-specific limitations availability polytene chromosomes favorable analysis. Recent molecular characterization both breakpoints presented opportunity develop a...
The preservation of a homomorphic sex-determining chromosome in some organisms without transformation into heteromorphic sex is long-standing enigma evolutionary biology. A dominant locus (or M-locus) an undifferentiated confers the male phenotype yellow fever mosquito Aedes aegypti. Genetic evidence suggests that M-locus nonrecombining region. However, molecular nature has not been characterized. Using recently developed approach based on Illumina sequencing and female genomic DNA, we...