A5000318694- T-cell and B-cell Immunology
- Lymphoma Diagnosis and Treatment
- Advanced Proteomics Techniques and Applications
- Chronic Lymphocytic Leukemia Research
- Metabolomics and Mass Spectrometry Studies
- Single-cell and spatial transcriptomics
- Cancer Immunotherapy and Biomarkers
- Genomics and Phylogenetic Studies
- Mosquito-borne diseases and control
- RNA and protein synthesis mechanisms
- Glaucoma and retinal disorders
- COVID-19 diagnosis using AI
- Ubiquitin and proteasome pathways
- Machine Learning in Bioinformatics
- Connexins and lens biology
- Tuberculosis Research and Epidemiology
- Viral Infections and Outbreaks Research
- Reproductive Biology and Fertility
- Disaster Response and Management
- Genetics, Bioinformatics, and Biomedical Research
- COVID-19 epidemiological studies
- Insect Resistance and Genetics
- RNA Research and Splicing
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
University of Florida
2014-2024
GlobeImmune (United States)
2020
Johns Hopkins Medicine
2016-2019
Johns Hopkins University
2009-2019
Indian Institute of Science Education and Research Pune
2019
Institute of Bioinformatics
2006-2016
University of the West Indies
2016
Orlando VA Medical Center
2016
University of Toledo
2016
The Ohio State University
2016
What started as a cluster of patients with mysterious respiratory illness in Wuhan, China, December 2019, was later determined to be coronavirus disease 2019 (COVID-19). The pathogen severe acute syndrome 2 (SARS-CoV-2), novel Betacoronavirus, subsequently isolated the causative agent. SARS-CoV-2 is transmitted by droplets and fomites presents clinically fever, fatigue, myalgias, conjunctivitis, anosmia, dysgeusia, sore throat, nasal congestion, cough, dyspnea, nausea, vomiting, and/or...
The study of the human urinary proteome has potential to offer significant insights into normal physiology as well disease pathology. information obtained from such studies could be applied diagnosis various diseases. high sensitivity, resolution, and mass accuracy latest generation spectrometers provides an opportunity accurately catalog proteins present in urine, including those at low levels. To this end, we carried out a comprehensive analysis healthy individuals using high-resolution...
Osteoarthritis is a chronic musculoskeletal disorder characterized mainly by progressive degradation of the hyaline cartilage. Patients with osteoarthritis often postpone seeking medical help, which results in diagnosis being made at an advanced stage cartilage destruction. Sustained efforts are needed to identify specific markers that might help early diagnosis, monitoring disease progression and improving therapeutic outcomes. We employed multipronged proteomic approach, included multiple...
The genome sequencing of H37Rv strain <i>Mycobacterium tuberculosis</i> was completed in 1998 followed by the whole a clinical isolate, CDC1551 2002. Since then, genomic sequences number other strains have become available making it one better studied pathogenic bacterial species at level. However, annotation its remains challenging because high GC content and dissimilarity to model prokaryotes. To this end, we carried out an in-depth proteogenomic analysis <i>M. using Fourier transform mass...
Polycystic ovary syndrome (PCOS), a major cause of anovulatory infertility, is characterized by arrested follicular growth. Altered protein levels in the fluid surrounding ovum may reflect molecular defects folliculogenesis these women.To identify differentially regulated proteins PCOS comparing repertoire with healthy women.The samples were collected from and normo-ovulatory women undergoing vitro fertilization. Follicular subjected to digestion using trypsin, resultant peptides labeled...
First reported in remote villages of Africa the 1970s, Ebolavirus was originally believed to be transmitted people from wild animals. (EBOV) causes a severe, frequently fatal hemorrhagic syndrome humans. Each outbreak over last three decades has perpetuated fear and economic turmoil among local regional populations Africa. Until now it been considered tragic malady confined largely isolated regions African continent, but is no longer so. The frequency outbreaks increased since 1970s. 2014...
Complementing genome sequence with deep transcriptome and proteome data could enable more accurate assembly annotation of newly sequenced genomes. Here, we provide a proof-of-concept an integrated approach for analysis the Anopheles stephensi, which is one most important vectors malaria parasite. To achieve broad coverage genes, carried out sequencing profiling multiple anatomically distinct sites. Based on transcriptomic alone, identified corrected 535 events incomplete involving 1196...
Cancer-associated mutations in the spliceosome gene SF3B1 create a neomorphic protein that produces aberrant mRNA splicing hundreds of genes, but ensuing biologic and therapeutic consequences this missplicing are not well understood. Here we have provided evidence by mutant altered transcriptome, proteome, metabolome human cells, leading to missplicing-associated downregulation metabolic decreased mitochondrial respiration, suppression serine synthesis pathway. We also found induces...
Accurate annotation of protein-coding genes is one the primary tasks upon completion whole genome sequencing any organism. In this study, we used an integrated transcriptomic and proteomic strategy to validate improve existing zebrafish annotation. We undertook high-resolution mass-spectrometry-based profiling 10 adult organs, fish body, two developmental stages (SAT line), in addition six organs. More than 7,000 proteins were identified from analyses, ∼69,000 high-confidence transcripts...
The aqueous humor is a colorless, transparent fluid that fills the anterior chamber of eye. It plays an important role in maintaining intraocular pressure and providing nourishment to lens cornea. constitution controlled by blood–aqueous barrier. Though this ocular has been extensively studied, its physiology still not completely understood. In study, samples were collected from 250 patients undergoing cataract surgery, subjected multiple fractionation strategies analyzed on Fourier...
The Ebola outbreak of 2014-2015 exacted a terrible toll on major countries West Africa. Latest estimates from the World Health Organization indicate that over 11,000 lives were lost to deadly virus since first documented case was officially recorded. However, significant progress in fight against made thanks combination globally-supported containment efforts, dissemination key information public, use modern technology resources better track spread outbreak, as well more effective active...
Anopheles gambiae is a major mosquito vector responsible for malaria transmission, whose genome sequence was reported in 2002. Genome annotation continuing effort, and many of the approximately 13,000 genes listed VectorBase are predictions that have still not been validated by any other method. To identify protein-coding An. based on its genomic sequence, we carried out deep proteomic analysis using high-resolution Fourier transform mass spectrometry both precursor fragment ions. Based...
Visceral leishmaniasis or kala azar is the most severe form of and caused by protozoan parasite Leishmania donovani . There no published report on L. genome sequence available till date, although sequences three related species are already available. Thus, we took a proteogenomic approach to identify proteins from two different life stages From our analysis promastigote (insect) amastigote (human) , identified total 22322 unique peptides homology‐based search against species. These were...
Breast cancer is the most prevalent in women worldwide. About 15-20% of all breast cancers do not express estrogen receptor, progesterone receptor or HER2 and hence are collectively classified as triple negative (TNBC). These tumors often relatively aggressive when compared to other types cancer, this issue compounded by lack effective targeted therapy. In our previous phosphoproteomic profiling effort, we identified non-receptor tyrosine kinase TNK2 activated a majority TNBC cell lines....
Cellular function and diversity are orchestrated by complex interactions of fundamental biomolecules including DNA, RNA proteins. Technological advances in genomics, epigenomics, transcriptomics proteomics have enabled massively parallel unbiased measurements. Such high-throughput technologies been extensively used to carry out broad, studies, particularly the context human diseases. Nevertheless, a unified analysis genome, epigenome, transcriptome proteome single cell type obtain coherent...
The ability to derive neural progenitors, differentiated neurons and glial cells from human embryonic stem (hESCs) with high efficiency holds promise for a number of clinical applications. However, investigating the temporal events is crucial defining underlying mechanisms that drive this process differentiation along different lineages. We carried out quantitative proteomic profiling using multiplexed approach capable analyzing eight samples simultaneously monitor dynamics protein abundance...
The ciliary body is the circumferential muscular tissue located just behind iris in anterior chamber of eye. It plays a pivotal role production aqueous humor, maintenance lens zonules and accommodation by changing shape crystalline lens. major target drugs against glaucoma as its inhibition leads to drop intraocular pressure. A molecular study could provide better understanding about pathophysiological processes that occur glaucoma. Thus far, no large-scale proteomic investigation has been...
Acute ischemic stroke (AIS) is a devastating cerebrovascular disorder that leads to permanent physical and neurological disabilities in adults worldwide. Proteins associated with pathogenesis may appear the serum of AIS patients due blood-brain barrier dysfunction, thus permitting development blood-based biomarkers for early diagnosis stroke. These could perhaps be an adjunct existing imaging modalities aid better management therapeutic intervention during course disease. For this...
The alarming global rise in fatalities from multidrug-resistant Staphylococcus aureus (S. aureus) infections has underscored a need to develop new therapies address this epidemic. Chemoproteomics is valuable identifying targets for drugs different human diseases including bacterial infections. Targeting functional cysteines particularly attractive, as they serve critical catalytic functions that enable survival. Here, we report an indole-based quinone epoxide scaffold with unique boat-like...
Cryptococcus neoformans, a basidiomycetous fungus of universal occurrence, is significant opportunistic human pathogen causing meningitis. Owing to an increase in the number immunosuppressed individuals along with emergence drug-resistant strains, C. neoformans gaining importance as pathogen. Although, whole genome sequencing three varieties has been completed recently, no global proteomic studies have yet reported.We performed comprehensive analysis var. grubii (Serotype A), which most...