A5036055830- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- Neural dynamics and brain function
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- Rabies epidemiology and control
- MicroRNA in disease regulation
- interferon and immune responses
- Insect and Arachnid Ecology and Behavior
- Neuroscience and Neuropharmacology Research
- Epigenetics and DNA Methylation
- Neuroinflammation and Neurodegeneration Mechanisms
- Plant and animal studies
- Chromosomal and Genetic Variations
- Insect and Pesticide Research
- Mesenchymal stem cell research
- Photoreceptor and optogenetics research
- Circular RNAs in diseases
- Molecular Biology Techniques and Applications
- Animal Virus Infections Studies
- Cancer Research and Treatments
- Acoustic Wave Resonator Technologies
- Bone and Dental Protein Studies
- RNA Interference and Gene Delivery
- Viral Infections and Outbreaks Research
Shenzhen University Health Science Center
2025
Zhuhai Hospital of Integrated Traditional Chinese and Western Medicine
2025
Southern Medical University
2025
Shenzhen Institutes of Advanced Technology
2024-2025
Chinese Academy of Sciences
2016-2025
Huazhong Agricultural University
2017-2024
Second Affiliated Hospital of Xi'an Jiaotong University
2014-2024
Zhejiang Chinese Medical University
2024
Aarhus University
2023
Shanghai Zhangjiang Laboratory
2021-2023
Neurotropic virus-based tracers have been extensively applied in mapping and manipulation of neural circuits. However, their neurotropic neurotoxic properties remain to be fully characterized.Through circuit tracing, we systematically compared the neurotropism discrepancy among different multi-trans-synaptic mono-synaptic retrograde viral including pseudorabies virus (PRV), rabies (RV), newly engineered retro adeno-associated (rAAV2-retro) tracers. The (single-cell) RNA sequencing analysis...
Bumblebees are a diverse group of globally important pollinators in natural ecosystems and for agricultural food production. With both eusocial solitary life-cycle phases, some social parasite species, they especially interesting models to understand evolution, behavior, ecology. Reports many species decline point pathogen transmission, habitat loss, pesticide usage, global climate change, as interconnected causes. These threats bumblebee diversity make our reliance on handful well-studied...
Abstract In the unprecedented single-cell sequencing and spatial multiomics era of biology, fluorescence in situ hybridization (FISH) technologies with higher sensitivity robustness, especially for detecting short RNAs other biomolecules, are greatly desired. Here, we develop robust multiplex π-FISH rainbow method to detect diverse biomolecules (DNA, RNA, proteins, neurotransmitters) individually or simultaneously high efficiency. This versatile is successfully applied gene expression...
Abstract Autonomic imbalance is an important characteristic of patients after myocardial infarction (MI) and adversely contributes to post‐MI cardiac remodeling ventricular arrhythmias (VAs). A previous study proved that optogenetic modulation could precisely inhibit sympathetic hyperactivity prevent acute ischemia‐induced VAs. Here, a wireless self‐powered system introduced, which achieves long‐term precise neuromodulation in ambulatory canines. The optical based on triboelectric...
Connectivity determines the function of neural circuits. Historically, circuit mapping has usually been viewed as a problem microscopy, but no current method can achieve high-throughput entire circuits with single neuron precision. Here we describe novel approach to determining connectivity. We propose BOINC ("barcoding individual neuronal connections"), for converting connectivity into form that be read out by DNA sequencing. The appeal using sequencing is its scale—sequencing billions...
Integration of human papillomavirus (HPV) DNA into the genome is a reputed key driver cervical cancer. However, effects HPV integration on chromatin structural organization and gene expression are largely unknown. We studied cohort 61 samples identified an hot spot in CCDC106 chromosome 19. then selected fresh cancer tissue that contained unique loci at with no episomal performed whole-genome, RNA, immunoprecipitation high-throughput conformation capture (Hi-C) sequencing to identify...
Acquisition of drug-resistant phenotypes is often associated with chemotherapy in osteosarcoma. A number studies have demonstrated a critical role for autophagy osteosarcoma development, therapy and drug resistance. However, the molecular mechanisms underlying autophagy-mediated resistance cells remain largely unknown. In present study, we determined microRNA-140 (miR-140-5p, miRBase ID: MIMAT0000431) expression induced by chemotherapeutic drugs cells. Then promotory miR-140-5p to...
Abstract Background Data visualization, especially the genome track plots, is crucial for genomics researchers to discover patterns in large-scale sequencing dataset. Although existing tools works well producing a normal view of input data, they are not convenient when users want create customized data representations. Such gap between visualization and processing, prevents uncover more hidden structure Results We developed CoolBox—an open-source toolkit visual analysis data. This...
Abstract Pyroptosis induced by the N-terminal gasdermin domain (GSDM NT ) holds great potential for anti-tumor therapy. However, due to extreme cytoxicity of GSDM , it is challenging efficiently produce and deliver into tumor cells. Here, we report development two strategies package recombinant adeno-associated virus (rAAV) expressing : 1) drive expression a mammal specific promoter in Sf9 insect cells avoid its expression; 2) co-infect rAAV-Cre revert express double-floxed inverted . We...
Abstract Immunocytes dynamically reprogram their gene expression profiles during differentiation and immunoresponse. However, the underlying mechanism remains elusive. Here, we develop a single-cell Hi-C method systematically delineate 3D genome dynamic epigenetic atlas of macrophages these processes. We propose “degree disorder” to measure organizational patterns inside topologically-associated domains, which is correlated with chromatin states, expression, structure variability in...
Human papillomavirus (HPV) is predominantly associated with HPV-related cancers, however, the precise mechanisms underlying HPV-host epigenetic architectures in HPV carcinogenesis remain elusive. Here, we employed high-throughput chromosome conformation capture (Hi-C) to comprehensively map HPV16/18-host chromatin interactions. Our study identified transcription factor Sp1 as a pivotal mediator programming By targeting Sp1, active histone modifications (H3K27ac, H3K4me1, and H3K4me3)...
Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating diseases affecting swine industry worldwide. To investigate role miRNAs in infection susceptibility PRRS virus (PRRSV), twenty-four miRNA libraries were constructed sequenced from PRRSV-infected mock-infected alveolar macrophages (PAMs) Meishan, Landrace, Pietrain Qingping pigs at 9 hours post (hpi), 36 hpi, 60 hpi. The let-7 family significantly differentially expressed between PAMs 4 pig...
MicroRNAs (miRs) serve a regulatory function in oxidative radical-mediated inflammation and apoptosis during ischemia/reperfusion (IR) injury. Lipocalin 2 (Lcn‑2), target protein of miR‑138, is widely involved the systemic response to IR The aim present study was investigate association between miR‑138 Lcn‑2 rat model cerebral (CIR) injury verify interaction PC12 cell hypoxia/reoxygenation Reverse transcription‑quantitative polymerase chain reaction western blot analysis were used detect...
The brain of the domestic pig (Sus scrofa domesticus) has drawn considerable attention due to its high similarities that humans. However, cellular compositions (PB) remain elusive. Here we investigated single-nucleus transcriptomic profiles five regions PB (frontal lobe, parietal temporal occipital and hypothalamus) identified 21 cell subpopulations. cross-species comparison mouse hypothalamus revealed shared specific gene expression patterns at single-cell resolution. Furthermore, types...
Objective To evaluate the application of Computed Tomography Angiography (CTA) combined with 3D-Slicer software reconstruction technology in diagnosis and preoperative planning intracranial aneurysms, to explore its positive significance improving surgical outcomes for patients. Methods From January 2021 December 2023, a total 24 patients diagnosed aneurysms (25 cases) underwent craniotomy clipping surgery. We utilized render fuse CTA image data, formulate individualized plans, including...
Ischemia-reperfusion (I/R) injury is a major contributor to myocardial dysfunction and tissue damage. A natural alkaloid-Berberine having wide range of pharmacological properties, has garnered interest for its potential cardioprotective properties. This study aimed investigate the protective effects berberine on in rat model ischemia-reperfusion injury. Additionally, explored role miR-184/NOTCH1 signaling pathway mediating these effects. Male Wistar rats were randomly assigned five groups:...
Osteoblasts and adipocytes are derived from a common precursor, mesenchymal stem cells (MSCs). Alterations in the normal fate of differentiating MSCs involved development obesity osteoporosis. Here, we report that viable motheaten (me(v)) mice, which deficient SH2-domain-containing phosphatase-1 (SHP1), develop osteoporosis spontaneously. Consistently, me(v)/me(v) mice exhibit significantly reduced osteogenic potential greatly increased adipogenic potential. When were transplanted into nude...