A5083790486- Microtubule and mitosis dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- Pancreatic function and diabetes
- Cellular Mechanics and Interactions
- Reproductive Biology and Fertility
- Genetics, Aging, and Longevity in Model Organisms
- Diabetes Management and Research
- Zebrafish Biomedical Research Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Diabetes and associated disorders
- Calcium signaling and nucleotide metabolism
- Photosynthetic Processes and Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Protein Degradation and Inhibitors
- Adenosine and Purinergic Signaling
- Histone Deacetylase Inhibitors Research
- Peptidase Inhibition and Analysis
- Educational Methods and Outcomes
- Neonatal and fetal brain pathology
- Mathematics Education and Pedagogy
Technische Universität Dresden
2020-2024
German Center for Diabetes Research
2020-2024
Heinrich Heine University Düsseldorf
2020-2024
Deutsches Diabetes-Zentrum e.V.
2020-2024
Paul Langerhans Institute Dresden
2020
Helmholtz Zentrum München
2020
Max Planck Institute of Molecular Cell Biology and Genetics
2017-2020
Hobart and William Smith Colleges
2015
Type 2 diabetes is characterized by peripheral insulin resistance and insufficient release from pancreatic islet β cells. However, the role sequence of cell dysfunction mass loss for reduced levels in type pathogenesis are unclear. Here, we exploit freshly explanted pancreas specimens metabolically phenotyped surgical patients using an situ tissue slice technology. This approach allows assessment volume function within samples stratified individuals. We show that, pre-diabetic, impaired...
In type 1 diabetes (T1D), autoimmune destruction of pancreatic β cells leads to insulin deficiency and loss glycemic control. However, knowledge about human pancreas pathophysiology in T1D remains incomplete. To address this limitation, we established a tissue slice platform donor organs with without diabetes, facilitating the first live cell studies pathogenesis our knowledge. We show that slices from organ donors allow thorough assessment processes critical for disease development,...
Centrosomes must resist microtubule-mediated forces for mitotic chromosome segregation. During exit, however, centrosomes are deformed and fractured by those same forces, which is a key step in centrosome disassembly. How the functional material properties of change throughout cell cycle, how they molecularly tuned, remain unknown. Here, we used optically induced flow perturbations to determine molecular basis strength ductility C. elegans embryos. We found that both declined sharply at...
Centrosomes are microtubule-nucleating organelles that facilitate chromosome segregation and cell division in metazoans. comprise centrioles organize a micron-scale mass of protein called pericentriolar material (PCM) from which microtubules nucleate. During each cycle, PCM accumulates around through phosphorylation-mediated assembly scaffold proteins. mitotic exit, swiftly disassembles by an unknown mechanism. Here, we used Caenorhabditis elegans embryos to determine the mechanism...
Abstract Blunted first-phase insulin secretion and deficiency are indicators of β cell dysfunction diabetes manifestation. Therefore, insights into molecular mechanisms that regulate homeostasis might provide entry sites to replenish content restore function. Here, we identify the inhibitory receptor (inceptor; encoded by gene IIR/ELAPOR1 ) as an insulin-binding regulates stores lysosomal degradation. Using human induced pluripotent stem (SC)-derived islets, show IIR knockout (KO) results in...
ABSTRACT Centrosomes are major microtubule-nucleating organelles that facilitate chromosome segregation and cell division in metazoans. comprise centrioles organize a micron-scale mass of protein called pericentriolar material (PCM) from which microtubules nucleate. During each cycle, PCM accumulates around through phosphorylation-mediated assembly scaffold proteins. mitotic exit, swiftly disassembles by an unknown mechanism. Here, we used Caenorhabditis elegans embryos to determine the...
Wound closure after a brain injury is critical for tissue restoration but this process still not well characterised at the level. We use live observation of wound in larval zebrafish inflicting stab to brain. demonstrate that closes first 24 hours by global contraction. Microglia accumulation point convergence precedes and computational modelling indicates physical traction microglia could lead closure. Indeed, genetically or pharmacologically depleting leads defective repair. Live...
Wound closure after brain injury is crucial for tissue restoration but remains poorly understood at the level. We investigated this process using in vivo observations of larval zebrafish injury. Our findings show that wound occurs within first 24 h through global contraction, as evidenced by live-imaging and drug inhibition studies. Microglia accumulate site before closure, computational models suggest their physical traction could drive process. Depleting microglia genetically or...
Gene expression is partly controlled by posttranscriptional modifications to histones. These either loosen the binding between histones and DNA, allowing for transcription, or tighten binding, which prevents transcription. Histone acetyltransferases (HATs) are responsible histone acetylation, while deacetylases (HDACs) remove acetyl groups involved in gene silencing. HDAC activity often upregulated cancer cells, leading repression of several genes that normally prevent tumor formation. Due...
ABSTRACT Centrosomes must resist microtubule-mediated forces for mitotic chromosome segregation. During exit, however, centrosomes are deformed and fractured by those same forces, which is a key step in centrosome disassembly. How the functional material properties of change throughout cell cycle, how they molecularly tuned remain unknown. Here, we used optically-induced flow perturbations to determine molecular basis strength ductility C. elegans embryos. We found that both declined sharply...